CHARACTERIZATION OF 18S RIBOSOMAL RNA FRAGMENT FROM <em>Solanum tuberosum</em> L. var. <em>Granola</em> POTATO
Potato (Solanum tuberosum L.) is a prime horticultural commodity. One of the varieties of potato that widely cultivated in Indonesia is Granola. This study characterized the variety Granola based on the 18S sequences of rRNA gene fragment. The 18S sequences were used to distinguish the Granola and d...
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| Format: | Article |
| Language: | Indonesian |
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Jenderal Soedirman University
2018-03-01
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| Series: | Scripta Biologica |
| Subjects: | |
| Online Access: | https://journal.bio.unsoed.ac.id/index.php/scribio/article/view/760 |
| _version_ | 1818243356182970368 |
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| author | Yunior William Susanto Hermin Pancasakti Kusumaningrum Siti Nur Jannah Sri Rustini |
| author_facet | Yunior William Susanto Hermin Pancasakti Kusumaningrum Siti Nur Jannah Sri Rustini |
| author_sort | Yunior William Susanto |
| collection | DOAJ |
| description | Potato (Solanum tuberosum L.) is a prime horticultural commodity. One of the varieties of potato that widely cultivated in Indonesia is Granola. This study characterized the variety Granola based on the 18S sequences of rRNA gene fragment. The 18S sequences were used to distinguish the Granola and determine the differentiating characters from other Solanum based on those sequences data. The characterization was completed in three main steps including DNA isolation from potato leaf using Doyle & Doyle method, amplification of the 18S gene fragment, and DNA sequencing. The amplification of 18S gene fragment by a PCR method obtained 528 bp sequences. The BLAST search using NCBI web service confirmed that Granola potato has 99% matching sequence with S. tuberosum. The phylogenetic reconstruction further indicates the S. tuberosum var. Granola used in this study deeply nested with the reference sequence X67238.1, a potato from Europe. |
| first_indexed | 2024-12-12T13:59:49Z |
| format | Article |
| id | doaj.art-70c6b8bee92341868808f66140b30d3d |
| institution | Directory Open Access Journal |
| issn | 2355-3138 |
| language | Indonesian |
| last_indexed | 2024-12-12T13:59:49Z |
| publishDate | 2018-03-01 |
| publisher | Jenderal Soedirman University |
| record_format | Article |
| series | Scripta Biologica |
| spelling | doaj.art-70c6b8bee92341868808f66140b30d3d2022-12-22T00:22:22ZindJenderal Soedirman UniversityScripta Biologica2355-31382018-03-015110.20884/1.sb.2018.5.1.760405CHARACTERIZATION OF 18S RIBOSOMAL RNA FRAGMENT FROM <em>Solanum tuberosum</em> L. var. <em>Granola</em> POTATOYunior William Susanto0Hermin Pancasakti Kusumaningrum1Siti Nur Jannah2Sri Rustini3Diponegoro UniversityDiponegoro UniversityDiponegoro UniversityBalai Pengkajian Teknologi Pertanian (BPTP) Jawa TengahPotato (Solanum tuberosum L.) is a prime horticultural commodity. One of the varieties of potato that widely cultivated in Indonesia is Granola. This study characterized the variety Granola based on the 18S sequences of rRNA gene fragment. The 18S sequences were used to distinguish the Granola and determine the differentiating characters from other Solanum based on those sequences data. The characterization was completed in three main steps including DNA isolation from potato leaf using Doyle & Doyle method, amplification of the 18S gene fragment, and DNA sequencing. The amplification of 18S gene fragment by a PCR method obtained 528 bp sequences. The BLAST search using NCBI web service confirmed that Granola potato has 99% matching sequence with S. tuberosum. The phylogenetic reconstruction further indicates the S. tuberosum var. Granola used in this study deeply nested with the reference sequence X67238.1, a potato from Europe.https://journal.bio.unsoed.ac.id/index.php/scribio/article/view/760Granolacharacterization18S rRNAsequencingphylogenetics |
| spellingShingle | Yunior William Susanto Hermin Pancasakti Kusumaningrum Siti Nur Jannah Sri Rustini CHARACTERIZATION OF 18S RIBOSOMAL RNA FRAGMENT FROM <em>Solanum tuberosum</em> L. var. <em>Granola</em> POTATO Scripta Biologica Granola characterization 18S rRNA sequencing phylogenetics |
| title | CHARACTERIZATION OF 18S RIBOSOMAL RNA FRAGMENT FROM <em>Solanum tuberosum</em> L. var. <em>Granola</em> POTATO |
| title_full | CHARACTERIZATION OF 18S RIBOSOMAL RNA FRAGMENT FROM <em>Solanum tuberosum</em> L. var. <em>Granola</em> POTATO |
| title_fullStr | CHARACTERIZATION OF 18S RIBOSOMAL RNA FRAGMENT FROM <em>Solanum tuberosum</em> L. var. <em>Granola</em> POTATO |
| title_full_unstemmed | CHARACTERIZATION OF 18S RIBOSOMAL RNA FRAGMENT FROM <em>Solanum tuberosum</em> L. var. <em>Granola</em> POTATO |
| title_short | CHARACTERIZATION OF 18S RIBOSOMAL RNA FRAGMENT FROM <em>Solanum tuberosum</em> L. var. <em>Granola</em> POTATO |
| title_sort | characterization of 18s ribosomal rna fragment from em solanum tuberosum em l var em granola em potato |
| topic | Granola characterization 18S rRNA sequencing phylogenetics |
| url | https://journal.bio.unsoed.ac.id/index.php/scribio/article/view/760 |
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