Structural confirmation of oligosaccharides newly isolated from sugar beet molasses
<p>Abstract</p> <p>Background</p> <p>Sugar beet molasses is a viscous by-product of the processing of sugar beets into sugar. The molasses is known to contain sucrose and raffinose, a typical trisaccharide, with a well-established structure. Although sugar beet molasses...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2012-08-01
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| Series: | Chemistry Central Journal |
| Online Access: | http://journal.chemistrycentral.com/content/6/1/89 |
| _version_ | 1818982663753564160 |
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| author | Abe Tatsuya Horiuchi Kenichi Kikuchi Hiroto Aritsuka Tsutomu Takata Yusuke Fukushi Eri Fukushi Yukiharu Kawabata Jun Ueno Keiji Onodera Shuichi Shiomi Norio |
| author_facet | Abe Tatsuya Horiuchi Kenichi Kikuchi Hiroto Aritsuka Tsutomu Takata Yusuke Fukushi Eri Fukushi Yukiharu Kawabata Jun Ueno Keiji Onodera Shuichi Shiomi Norio |
| author_sort | Abe Tatsuya |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Sugar beet molasses is a viscous by-product of the processing of sugar beets into sugar. The molasses is known to contain sucrose and raffinose, a typical trisaccharide, with a well-established structure. Although sugar beet molasses contains various other oligosaccharides as well, the structures of those oligosaccharides have not been examined in detail. The purpose of this study was isolation and structural confirmation of these other oligosaccharides found in sugar beet molasses.</p> <p>Results</p> <p>Four oligosaccharides were newly isolated from sugar beet molasses using high-performance liquid chromatography (HPLC) and carbon-Celite column chromatography. Structural confirmation of the saccharides was provided by methylation analysis, matrix-assisted laser desorption/ionaization time of flight mass spectrometry (MALDI-TOF-MS), and nuclear magnetic resonance (NMR) measurements.</p> <p>Conclusion</p> <p>The following oligosaccharides were identified in sugar beet molasses: β-D-galactopyranosyl-(1- > 6)-β-D-fructofuranosyl-(2 <-> 1)-α-D-glucopyranoside (named β-planteose), α-D-galactopyranosyl-(1- > 1)-β-D-fructofuranosyl-(2 <-> 1)-α-D-glucopyranoside (named1-planteose), α-D-glucopyranosyl-(1- > 6)-α-D-glucopyranosyl-(1 <-> 2)-β-D-fructofuranoside (theanderose), and β-D-glucopyranosyl-(1- > 3)-α-D-glucopyranosyl-(1 <-> 2)-β-D-fructofuranoside (laminaribiofructose). 1-planteose and laminaribiofructose were isolated from natural sources for the first time.</p> |
| first_indexed | 2024-12-20T17:50:48Z |
| format | Article |
| id | doaj.art-70c73bbe164e4e03a516d00c1bd85afe |
| institution | Directory Open Access Journal |
| issn | 1752-153X |
| language | English |
| last_indexed | 2024-12-20T17:50:48Z |
| publishDate | 2012-08-01 |
| publisher | BMC |
| record_format | Article |
| series | Chemistry Central Journal |
| spelling | doaj.art-70c73bbe164e4e03a516d00c1bd85afe2022-12-21T19:30:52ZengBMCChemistry Central Journal1752-153X2012-08-01618910.1186/1752-153X-6-89Structural confirmation of oligosaccharides newly isolated from sugar beet molassesAbe TatsuyaHoriuchi KenichiKikuchi HirotoAritsuka TsutomuTakata YusukeFukushi EriFukushi YukiharuKawabata JunUeno KeijiOnodera ShuichiShiomi Norio<p>Abstract</p> <p>Background</p> <p>Sugar beet molasses is a viscous by-product of the processing of sugar beets into sugar. The molasses is known to contain sucrose and raffinose, a typical trisaccharide, with a well-established structure. Although sugar beet molasses contains various other oligosaccharides as well, the structures of those oligosaccharides have not been examined in detail. The purpose of this study was isolation and structural confirmation of these other oligosaccharides found in sugar beet molasses.</p> <p>Results</p> <p>Four oligosaccharides were newly isolated from sugar beet molasses using high-performance liquid chromatography (HPLC) and carbon-Celite column chromatography. Structural confirmation of the saccharides was provided by methylation analysis, matrix-assisted laser desorption/ionaization time of flight mass spectrometry (MALDI-TOF-MS), and nuclear magnetic resonance (NMR) measurements.</p> <p>Conclusion</p> <p>The following oligosaccharides were identified in sugar beet molasses: β-D-galactopyranosyl-(1- > 6)-β-D-fructofuranosyl-(2 <-> 1)-α-D-glucopyranoside (named β-planteose), α-D-galactopyranosyl-(1- > 1)-β-D-fructofuranosyl-(2 <-> 1)-α-D-glucopyranoside (named1-planteose), α-D-glucopyranosyl-(1- > 6)-α-D-glucopyranosyl-(1 <-> 2)-β-D-fructofuranoside (theanderose), and β-D-glucopyranosyl-(1- > 3)-α-D-glucopyranosyl-(1 <-> 2)-β-D-fructofuranoside (laminaribiofructose). 1-planteose and laminaribiofructose were isolated from natural sources for the first time.</p>http://journal.chemistrycentral.com/content/6/1/89 |
| spellingShingle | Abe Tatsuya Horiuchi Kenichi Kikuchi Hiroto Aritsuka Tsutomu Takata Yusuke Fukushi Eri Fukushi Yukiharu Kawabata Jun Ueno Keiji Onodera Shuichi Shiomi Norio Structural confirmation of oligosaccharides newly isolated from sugar beet molasses Chemistry Central Journal |
| title | Structural confirmation of oligosaccharides newly isolated from sugar beet molasses |
| title_full | Structural confirmation of oligosaccharides newly isolated from sugar beet molasses |
| title_fullStr | Structural confirmation of oligosaccharides newly isolated from sugar beet molasses |
| title_full_unstemmed | Structural confirmation of oligosaccharides newly isolated from sugar beet molasses |
| title_short | Structural confirmation of oligosaccharides newly isolated from sugar beet molasses |
| title_sort | structural confirmation of oligosaccharides newly isolated from sugar beet molasses |
| url | http://journal.chemistrycentral.com/content/6/1/89 |
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