An immunoenzymatic assay for the diagnosis of hepatitis A utilising immunoglobulin Y
The detection of anti-hepatitis A virus (HAV) antibody levels by diagnostic kits in the convalescent period of disease generally use immunoglobulin G (IgG), which is expensive. An alternative to IgG is immunoglobulin Y (IgY), an immunoglobulin antibody encountered in birds and reptiles. The aim of t...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2012-11-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762012000700022&lng=en&tlng=en |
| _version_ | 1797757120578322432 |
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| author | Alexandre dos Santos da Silva Gentil Arthur Lins Bentes Mendonça de Vasconcelos Livia Abud Kappel Marcelo Alves Pinto Vanessa Salete de Paula |
| author_facet | Alexandre dos Santos da Silva Gentil Arthur Lins Bentes Mendonça de Vasconcelos Livia Abud Kappel Marcelo Alves Pinto Vanessa Salete de Paula |
| author_sort | Alexandre dos Santos da Silva |
| collection | DOAJ |
| description | The detection of anti-hepatitis A virus (HAV) antibody levels by diagnostic kits in the convalescent period of disease generally use immunoglobulin G (IgG), which is expensive. An alternative to IgG is immunoglobulin Y (IgY), an immunoglobulin antibody encountered in birds and reptiles. The aim of this study was to develop a competitive immunoenzymatic assay to measure total anti-HAV antibody levels using anti-HAV IgY as the capture and conjugated immunoglobulins. For this purpose, anti-HAV IgY was conjugated to horseradish peroxidase (HRP) and the optimal dilution of HRP-conjugated antibodies was evaluated to establish the competitive immuneenzymatic assay. The results obtained from our "in-house" assay were plotted on a receiver operator curve, which showed a sensitivity of 95% and a specificity of 98.8%, demonstrating that a competitive anti-HAV IgY immunoenzymatic assay developed "in house" could be used as an alternative to commercial assays that utilise IgG. |
| first_indexed | 2024-03-12T18:11:23Z |
| format | Article |
| id | doaj.art-70c8175ddf4f48e2a9689f5e8d3f0ed1 |
| institution | Directory Open Access Journal |
| issn | 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T18:11:23Z |
| publishDate | 2012-11-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-70c8175ddf4f48e2a9689f5e8d3f0ed12023-08-02T09:15:27ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz1678-80602012-11-01107796096310.1590/S0074-02762012000700022S0074-02762012000700022An immunoenzymatic assay for the diagnosis of hepatitis A utilising immunoglobulin YAlexandre dos Santos da Silva0Gentil Arthur Lins Bentes Mendonça de Vasconcelos1Livia Abud Kappel2Marcelo Alves Pinto3Vanessa Salete de Paula4Fundação Oswaldo CruzFundação Oswaldo CruzFundação Oswaldo CruzFundação Oswaldo CruzFundação Oswaldo CruzThe detection of anti-hepatitis A virus (HAV) antibody levels by diagnostic kits in the convalescent period of disease generally use immunoglobulin G (IgG), which is expensive. An alternative to IgG is immunoglobulin Y (IgY), an immunoglobulin antibody encountered in birds and reptiles. The aim of this study was to develop a competitive immunoenzymatic assay to measure total anti-HAV antibody levels using anti-HAV IgY as the capture and conjugated immunoglobulins. For this purpose, anti-HAV IgY was conjugated to horseradish peroxidase (HRP) and the optimal dilution of HRP-conjugated antibodies was evaluated to establish the competitive immuneenzymatic assay. The results obtained from our "in-house" assay were plotted on a receiver operator curve, which showed a sensitivity of 95% and a specificity of 98.8%, demonstrating that a competitive anti-HAV IgY immunoenzymatic assay developed "in house" could be used as an alternative to commercial assays that utilise IgG.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762012000700022&lng=en&tlng=enIgYhepatitis AELISA |
| spellingShingle | Alexandre dos Santos da Silva Gentil Arthur Lins Bentes Mendonça de Vasconcelos Livia Abud Kappel Marcelo Alves Pinto Vanessa Salete de Paula An immunoenzymatic assay for the diagnosis of hepatitis A utilising immunoglobulin Y Memorias do Instituto Oswaldo Cruz IgY hepatitis A ELISA |
| title | An immunoenzymatic assay for the diagnosis of hepatitis A utilising immunoglobulin Y |
| title_full | An immunoenzymatic assay for the diagnosis of hepatitis A utilising immunoglobulin Y |
| title_fullStr | An immunoenzymatic assay for the diagnosis of hepatitis A utilising immunoglobulin Y |
| title_full_unstemmed | An immunoenzymatic assay for the diagnosis of hepatitis A utilising immunoglobulin Y |
| title_short | An immunoenzymatic assay for the diagnosis of hepatitis A utilising immunoglobulin Y |
| title_sort | immunoenzymatic assay for the diagnosis of hepatitis a utilising immunoglobulin y |
| topic | IgY hepatitis A ELISA |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762012000700022&lng=en&tlng=en |
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