Evaluation of reference genes for insect olfaction studies

Evaluation of reference genes for insect olfaction studies

Abstract Background Quantitative reverse transcription PCR (qRT-PCR) is a robust and accessible method to assay gene expression and to infer gene regulation. Being a chain of procedures, this technique is subject to systematic error due to biological and technical limitations mainly set by the start...

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Bibliographic Details
Main Authors: Bonaventure Aman Omondi, Jose Manuel Latorre-Estivalis, Ivana Helena Rocha Oliveira, Rickard Ignell, Marcelo Gustavo Lorenzo
Format: Article
Language:English
Published: BMC 2015-04-01
Series:Parasites & Vectors
Subjects:
RT-qPCR
Reference genes
Normalization process
Olfaction and triatomines
Online Access:https://doi.org/10.1186/s13071-015-0862-x

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