Comparative in vivo antiangiogenic effects of calreticulin from Trypanosoma cruzi and Homo sapiens sapiens
Angiogenesis is a complex multi-step process of neovascularization arising from preexisting blood vessels whose generation is regulated by pro- and anti-angiogenic factors. Both Trypanosoma cruzi calreticulin (TcCRT) and its human counterpart (HuCRT) are antiangiogenic. This is the first report wher...
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| Format: | Article |
| Language: | English |
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BMC
2010-01-01
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| Series: | Biological Research |
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| Online Access: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000300004 |
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| author | Victor Toledo Galia Ramírez Carolina Valck Nandy López Carolina H Ribeiro Ismael Maldonado Lorena Aguilar David Lemus Arturo Ferreira |
| author_facet | Victor Toledo Galia Ramírez Carolina Valck Nandy López Carolina H Ribeiro Ismael Maldonado Lorena Aguilar David Lemus Arturo Ferreira |
| author_sort | Victor Toledo |
| collection | DOAJ |
| description | Angiogenesis is a complex multi-step process of neovascularization arising from preexisting blood vessels whose generation is regulated by pro- and anti-angiogenic factors. Both Trypanosoma cruzi calreticulin (TcCRT) and its human counterpart (HuCRT) are antiangiogenic. This is the first report where the TcCRT and HuCRT anti-angiogenic properties are compared in vivo. In the chick embryonic chorioallantoid membrane assay (CAM) and at equimolar concentrations, TcCRT displayed significantly higher antiangiogenic activities than its human counterpart. LPS had marginal effects at the concentrations present in the recombinant protein preparations and the TcCRT antiangiogenic effects were largely inhibited by specific polyclonal antibodies, thus, reinforcing the fact that the observed TcCRT effects can be attributed to the parasite-derived molecule and not to the endotoxin. The antiangiogenic TcCRT effects correlate with its anti-tumor in vivo effects, as recently shown in our laboratory. |
| first_indexed | 2024-12-11T06:17:34Z |
| format | Article |
| id | doaj.art-70ca5568f853431ba2c4fba4971ddaf7 |
| institution | Directory Open Access Journal |
| issn | 0716-9760 0717-6287 |
| language | English |
| last_indexed | 2024-12-11T06:17:34Z |
| publishDate | 2010-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Biological Research |
| spelling | doaj.art-70ca5568f853431ba2c4fba4971ddaf72022-12-22T01:17:56ZengBMCBiological Research0716-97600717-62872010-01-01433287289Comparative in vivo antiangiogenic effects of calreticulin from Trypanosoma cruzi and Homo sapiens sapiensVictor ToledoGalia RamírezCarolina ValckNandy LópezCarolina H RibeiroIsmael MaldonadoLorena AguilarDavid LemusArturo FerreiraAngiogenesis is a complex multi-step process of neovascularization arising from preexisting blood vessels whose generation is regulated by pro- and anti-angiogenic factors. Both Trypanosoma cruzi calreticulin (TcCRT) and its human counterpart (HuCRT) are antiangiogenic. This is the first report where the TcCRT and HuCRT anti-angiogenic properties are compared in vivo. In the chick embryonic chorioallantoid membrane assay (CAM) and at equimolar concentrations, TcCRT displayed significantly higher antiangiogenic activities than its human counterpart. LPS had marginal effects at the concentrations present in the recombinant protein preparations and the TcCRT antiangiogenic effects were largely inhibited by specific polyclonal antibodies, thus, reinforcing the fact that the observed TcCRT effects can be attributed to the parasite-derived molecule and not to the endotoxin. The antiangiogenic TcCRT effects correlate with its anti-tumor in vivo effects, as recently shown in our laboratory.http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000300004CalreticulinT. cruzianti-angiogenesis |
| spellingShingle | Victor Toledo Galia Ramírez Carolina Valck Nandy López Carolina H Ribeiro Ismael Maldonado Lorena Aguilar David Lemus Arturo Ferreira Comparative in vivo antiangiogenic effects of calreticulin from Trypanosoma cruzi and Homo sapiens sapiens Biological Research Calreticulin T. cruzi anti-angiogenesis |
| title | Comparative in vivo antiangiogenic effects of calreticulin from Trypanosoma cruzi and Homo sapiens sapiens |
| title_full | Comparative in vivo antiangiogenic effects of calreticulin from Trypanosoma cruzi and Homo sapiens sapiens |
| title_fullStr | Comparative in vivo antiangiogenic effects of calreticulin from Trypanosoma cruzi and Homo sapiens sapiens |
| title_full_unstemmed | Comparative in vivo antiangiogenic effects of calreticulin from Trypanosoma cruzi and Homo sapiens sapiens |
| title_short | Comparative in vivo antiangiogenic effects of calreticulin from Trypanosoma cruzi and Homo sapiens sapiens |
| title_sort | comparative in vivo antiangiogenic effects of calreticulin from trypanosoma cruzi and homo sapiens sapiens |
| topic | Calreticulin T. cruzi anti-angiogenesis |
| url | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000300004 |
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