Genome Sequence and Characterization of a Xanthorhodopsin-Containing, Aerobic Anoxygenic Phototrophic <i>Rhodobacter</i> Species, Isolated from Mesophilic Conditions at Yellowstone National Park
The genus <i>Rhodobacter</i> consists of purple nonsulfur photosynthetic alphaproteobacteria known for their diverse metabolic capabilities. Here, we report the genome sequence and initial characterization of a novel <i>Rhodobacter</i> species, strain M37P, isolated from Mush...
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| Format: | Article |
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MDPI AG
2022-06-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/10/6/1169 |
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| author | John A. Kyndt Sydney Robertson Isabella B. Shoffstall Robert F. Ramaley Terrance E. Meyer |
| author_facet | John A. Kyndt Sydney Robertson Isabella B. Shoffstall Robert F. Ramaley Terrance E. Meyer |
| author_sort | John A. Kyndt |
| collection | DOAJ |
| description | The genus <i>Rhodobacter</i> consists of purple nonsulfur photosynthetic alphaproteobacteria known for their diverse metabolic capabilities. Here, we report the genome sequence and initial characterization of a novel <i>Rhodobacter</i> species, strain M37P, isolated from Mushroom hot spring runoff in Yellowstone National Park at 37 °C. Genome-based analyses and initial growth characteristics helped to define the differentiating characteristics of this species and identified it as an aerobic anoxygenic phototroph (AAP). This is the first AAP identified in the genus <i>Rhodobacter</i>. Strain M37P has a pinkish-red pigmentation that is present under aerobic dark conditions but disappears under light incubation. Whole genome-based analysis and average nucleotide identity (ANI) comparison indicate that strain M37P belongs to a specific clade of recently identified species that are genetically and physiologically unique from other representative <i>Rhodobacter</i> species. The genome encodes a unique xanthorhodopsin, not found in any other <i>Rhodobacter</i> species, which may be responsible for the pinkish-red pigmentation. These analyses indicates that strain M37P is a unique species that is well-adapted to optimized growth in the Yellowstone hot spring runoff, for which we propose the name <i>Rhodobacter calidifons</i> sp. nov. |
| first_indexed | 2024-03-09T23:00:05Z |
| format | Article |
| id | doaj.art-70cf2124440b407dbf19484084ba1c48 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T23:00:05Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-70cf2124440b407dbf19484084ba1c482023-11-23T18:04:08ZengMDPI AGMicroorganisms2076-26072022-06-01106116910.3390/microorganisms10061169Genome Sequence and Characterization of a Xanthorhodopsin-Containing, Aerobic Anoxygenic Phototrophic <i>Rhodobacter</i> Species, Isolated from Mesophilic Conditions at Yellowstone National ParkJohn A. Kyndt0Sydney Robertson1Isabella B. Shoffstall2Robert F. Ramaley3Terrance E. Meyer4College of Science and Technology, Bellevue University, Bellevue, NE 68005, USACollege of Science and Technology, Bellevue University, Bellevue, NE 68005, USADepartment of Chemistry and Biochemistry, New Mexico State University, Las Cruces, NM 88003, USADepartment of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, NE 68198, USADepartment of Chemistry and Biochemistry, The University of Arizona, Tucson, AZ 85721, USAThe genus <i>Rhodobacter</i> consists of purple nonsulfur photosynthetic alphaproteobacteria known for their diverse metabolic capabilities. Here, we report the genome sequence and initial characterization of a novel <i>Rhodobacter</i> species, strain M37P, isolated from Mushroom hot spring runoff in Yellowstone National Park at 37 °C. Genome-based analyses and initial growth characteristics helped to define the differentiating characteristics of this species and identified it as an aerobic anoxygenic phototroph (AAP). This is the first AAP identified in the genus <i>Rhodobacter</i>. Strain M37P has a pinkish-red pigmentation that is present under aerobic dark conditions but disappears under light incubation. Whole genome-based analysis and average nucleotide identity (ANI) comparison indicate that strain M37P belongs to a specific clade of recently identified species that are genetically and physiologically unique from other representative <i>Rhodobacter</i> species. The genome encodes a unique xanthorhodopsin, not found in any other <i>Rhodobacter</i> species, which may be responsible for the pinkish-red pigmentation. These analyses indicates that strain M37P is a unique species that is well-adapted to optimized growth in the Yellowstone hot spring runoff, for which we propose the name <i>Rhodobacter calidifons</i> sp. nov.https://www.mdpi.com/2076-2607/10/6/1169aerobic anoxygenic phototrophsAAP<i>Rhodobacter</i>purple nonsulfur bacteriahot springYellowstone |
| spellingShingle | John A. Kyndt Sydney Robertson Isabella B. Shoffstall Robert F. Ramaley Terrance E. Meyer Genome Sequence and Characterization of a Xanthorhodopsin-Containing, Aerobic Anoxygenic Phototrophic <i>Rhodobacter</i> Species, Isolated from Mesophilic Conditions at Yellowstone National Park Microorganisms aerobic anoxygenic phototrophs AAP <i>Rhodobacter</i> purple nonsulfur bacteria hot spring Yellowstone |
| title | Genome Sequence and Characterization of a Xanthorhodopsin-Containing, Aerobic Anoxygenic Phototrophic <i>Rhodobacter</i> Species, Isolated from Mesophilic Conditions at Yellowstone National Park |
| title_full | Genome Sequence and Characterization of a Xanthorhodopsin-Containing, Aerobic Anoxygenic Phototrophic <i>Rhodobacter</i> Species, Isolated from Mesophilic Conditions at Yellowstone National Park |
| title_fullStr | Genome Sequence and Characterization of a Xanthorhodopsin-Containing, Aerobic Anoxygenic Phototrophic <i>Rhodobacter</i> Species, Isolated from Mesophilic Conditions at Yellowstone National Park |
| title_full_unstemmed | Genome Sequence and Characterization of a Xanthorhodopsin-Containing, Aerobic Anoxygenic Phototrophic <i>Rhodobacter</i> Species, Isolated from Mesophilic Conditions at Yellowstone National Park |
| title_short | Genome Sequence and Characterization of a Xanthorhodopsin-Containing, Aerobic Anoxygenic Phototrophic <i>Rhodobacter</i> Species, Isolated from Mesophilic Conditions at Yellowstone National Park |
| title_sort | genome sequence and characterization of a xanthorhodopsin containing aerobic anoxygenic phototrophic i rhodobacter i species isolated from mesophilic conditions at yellowstone national park |
| topic | aerobic anoxygenic phototrophs AAP <i>Rhodobacter</i> purple nonsulfur bacteria hot spring Yellowstone |
| url | https://www.mdpi.com/2076-2607/10/6/1169 |
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