Albumin Nano-Encapsulation of Piceatannol Enhances Its Anticancer Potential in Colon Cancer Via Downregulation of Nuclear p65 and HIF-1α
Piceatannol (PIC) is known to have anticancer activity, which has been attributed to its ability to block the proliferation of cancer cells via suppression of the NF-kB signaling pathway. However, its effect on hypoxia-inducible factor (HIF) is not well known in cancer. In this study, PIC was loaded...
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MDPI AG
2020-01-01
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| Series: | Cancers |
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| author | Alaa A. A. Aljabali Hamid A. Bakshi Faruck L. Hakkim Yusuf A. Haggag Khalid M. Al-Batanyeh Mazhar S. Al Zoubi Bahaa Al-Trad Mohamed M. Nasef Saurabh Satija Meenu Mehta Kavita Pabreja Vijay Mishra Mohammed Khan Salem Abobaker Ibrahim M. Azzouz Harish Dureja Ritesh M. Pabari Ashref Ali K. Dardouri Prashant Kesharwani Gaurav Gupta Shakti Dhar Shukla Parteek Prasher Nitin B. Charbe Poonam Negi Deepak N. Kapoor Dinesh Kumar Chellappan Mateus Webba da Silva Paul Thompson Kamal Dua Paul McCarron Murtaza M. Tambuwala |
| author_facet | Alaa A. A. Aljabali Hamid A. Bakshi Faruck L. Hakkim Yusuf A. Haggag Khalid M. Al-Batanyeh Mazhar S. Al Zoubi Bahaa Al-Trad Mohamed M. Nasef Saurabh Satija Meenu Mehta Kavita Pabreja Vijay Mishra Mohammed Khan Salem Abobaker Ibrahim M. Azzouz Harish Dureja Ritesh M. Pabari Ashref Ali K. Dardouri Prashant Kesharwani Gaurav Gupta Shakti Dhar Shukla Parteek Prasher Nitin B. Charbe Poonam Negi Deepak N. Kapoor Dinesh Kumar Chellappan Mateus Webba da Silva Paul Thompson Kamal Dua Paul McCarron Murtaza M. Tambuwala |
| author_sort | Alaa A. A. Aljabali |
| collection | DOAJ |
| description | Piceatannol (PIC) is known to have anticancer activity, which has been attributed to its ability to block the proliferation of cancer cells via suppression of the NF-kB signaling pathway. However, its effect on hypoxia-inducible factor (HIF) is not well known in cancer. In this study, PIC was loaded into bovine serum albumin (BSA) by desolvation method as PIC−BSA nanoparticles (NPs). These PIC−BSA nanoparticles were assessed for in vitro cytotoxicity, migration, invasion, and colony formation studies and levels of p65 and HIF-1α. Our results indicate that PIC−BSA NPs were more effective in downregulating the expression of nuclear p65 and HIF-1α in colon cancer cells as compared to free PIC. We also observed a significant reduction in inflammation induced by chemical colitis in mice by PIC−BSA NPs. Furthermore, a significant reduction in tumor size and number of colon tumors was also observed in the murine model of colitis-associated colorectal cancer, when treated with PIC−BSA NPs as compared to free PIC. The overall results indicate that PIC, when formulated as PIC−BSA NPs, enhances its therapeutic potential. Our work could prompt further research in using natural anticancer agents as nanoparticels with possible human clinical trails. This could lead to the development of a new line of safe and effective therapeutics for cancer patients. |
| first_indexed | 2024-03-12T19:14:04Z |
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| institution | Directory Open Access Journal |
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| language | English |
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| publishDate | 2020-01-01 |
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| spelling | doaj.art-70d0abc4efa34d3e84b6a2915834fb0a2023-08-02T05:37:07ZengMDPI AGCancers2072-66942020-01-0112111310.3390/cancers12010113cancers12010113Albumin Nano-Encapsulation of Piceatannol Enhances Its Anticancer Potential in Colon Cancer Via Downregulation of Nuclear p65 and HIF-1αAlaa A. A. Aljabali0Hamid A. Bakshi1Faruck L. Hakkim2Yusuf A. Haggag3Khalid M. Al-Batanyeh4Mazhar S. Al Zoubi5Bahaa Al-Trad6Mohamed M. Nasef7Saurabh Satija8Meenu Mehta9Kavita Pabreja10Vijay Mishra11Mohammed Khan12Salem Abobaker13Ibrahim M. Azzouz14Harish Dureja15Ritesh M. Pabari16Ashref Ali K. Dardouri17Prashant Kesharwani18Gaurav Gupta19Shakti Dhar Shukla20Parteek Prasher21Nitin B. Charbe22Poonam Negi23Deepak N. Kapoor24Dinesh Kumar Chellappan25Mateus Webba da Silva26Paul Thompson27Kamal Dua28Paul McCarron29Murtaza M. Tambuwala30Department of Pharmaceutical Sciences, Yarmouk University—Faculty of Pharmacy, Irbid 566, JordanSchool of Pharmacy and Pharmaceutical Science, Ulster University, Coleraine BT52 1SA, Northern Ireland, UKDepartment of Mathematics and Sciences, College of Arts and Applied Sciences Dhofar University Salalah, Salalah 211, OmanDepartment of Pharmaceutical Technology, Faculty of Pharmacy, University of Tanta, Tanta 31111, EgyptDepartment of Biological Sciences, Yarmouk University—Faculty of Science, Irbid 566, JordanDepartment of Basic Medical Sciences, Yarmouk University—Faculty of Medicine, Irbid 566, JordanDepartment of Biological Sciences, Yarmouk University—Faculty of Science, Irbid 566, JordanDepartment of Pharmacy and Biomedical Sciences, School of Applied Sciences, University of Huddersfield, Queensgate, Huddersfield HD1 3DH, UKSchool of Pharmaceutical Sciences, Lovely Professional University, Phagwara, Punjab 144411, IndiaSchool of Pharmaceutical Sciences, Lovely Professional University, Phagwara, Punjab 144411, IndiaSchool of Pharmaceutical Sciences, Lovely Professional University, Phagwara, Punjab 144411, IndiaSchool of Pharmaceutical Sciences, Lovely Professional University, Phagwara, Punjab 144411, IndiaSchool of Pharmacy and Pharmaceutical Science, Ulster University, Coleraine BT52 1SA, Northern Ireland, UKDepartment of Gynecology, European Competence Center for Ovarian Cancer, Campus Virchow, Klinikum Charite-Universitatmedizin Berlin, augustenburger Platz 1, 13353 Berlin, GermanyDepartment of Dermatology, Venerology, and allergology, Charite—Universitatsmedizin Berlin, Corporate Member of Freie Universitat Berlin, Chariteplatz1, 10117 Berlin, GermanyDepartment of Pharmaceutical Sciences, Maharshi Dayanand University, Rohtak 124001, IndiaSchool of Pharmacy, Royal College of Surgeons in Ireland, Dublin-09 D02 YN77, IrelandDepartment of Forensic Science, School of Applied Science, Huddersfield University, Queensgate, Huddersfield HD1 3DH, UKDepartment of Pharmaceutics, School of Pharmaceutical Education and Research, Jamia Hamdard, New Delhi 110062, IndiaSchool of Pharmacy, Suresh Gyan Vihar University, Jagatpura, Jaipur 302017, IndiaPriority Research Centre for Healthy Lungs, Hunter Medical Research Institute (HMRI) and School of Biomedical Sciences and Pharmacy, University of Newcastle, Callaghan, NSW 230, AustraliaDepartment of Chemistry, University of Petroleum & Energy Studies, Dehradun 248007, IndiaDepartamento de Química Orgánica, Facultad de Química y de Farmacia, Pontificia Universidad Católica de Chile, Av. Vicuña McKenna 4860, 7820436, Macul, Santiago 4860, ChileSchool of Pharmaceutical Sciences, Shoolini University of Biotechnology and Management Sciences, Solan, India 173229, IndiaSchool of Pharmaceutical Sciences, Shoolini University of Biotechnology and Management Sciences, Solan, India 173229, IndiaDepartment of Life Sciences, School of Pharmacy, International Medical University, Bukit Jalil, Kuala Lumpur 57000, MalaysiaSchool of Pharmacy and Pharmaceutical Science, Ulster University, Coleraine BT52 1SA, Northern Ireland, UKSchool of Biomedical Sciences, University of Ulster, Coleraine BT52 1SA, UKPriority Research Centre for Healthy Lungs, Hunter Medical Research Institute (HMRI) and School of Biomedical Sciences and Pharmacy, University of Newcastle, Callaghan, NSW 230, AustraliaSchool of Pharmacy and Pharmaceutical Science, Ulster University, Coleraine BT52 1SA, Northern Ireland, UKSchool of Pharmacy and Pharmaceutical Science, Ulster University, Coleraine BT52 1SA, Northern Ireland, UKPiceatannol (PIC) is known to have anticancer activity, which has been attributed to its ability to block the proliferation of cancer cells via suppression of the NF-kB signaling pathway. However, its effect on hypoxia-inducible factor (HIF) is not well known in cancer. In this study, PIC was loaded into bovine serum albumin (BSA) by desolvation method as PIC−BSA nanoparticles (NPs). These PIC−BSA nanoparticles were assessed for in vitro cytotoxicity, migration, invasion, and colony formation studies and levels of p65 and HIF-1α. Our results indicate that PIC−BSA NPs were more effective in downregulating the expression of nuclear p65 and HIF-1α in colon cancer cells as compared to free PIC. We also observed a significant reduction in inflammation induced by chemical colitis in mice by PIC−BSA NPs. Furthermore, a significant reduction in tumor size and number of colon tumors was also observed in the murine model of colitis-associated colorectal cancer, when treated with PIC−BSA NPs as compared to free PIC. The overall results indicate that PIC, when formulated as PIC−BSA NPs, enhances its therapeutic potential. Our work could prompt further research in using natural anticancer agents as nanoparticels with possible human clinical trails. This could lead to the development of a new line of safe and effective therapeutics for cancer patients.https://www.mdpi.com/2072-6694/12/1/113piceatannolcolon canceralbumin nanoparticlesnuclear p65hif-1α |
| spellingShingle | Alaa A. A. Aljabali Hamid A. Bakshi Faruck L. Hakkim Yusuf A. Haggag Khalid M. Al-Batanyeh Mazhar S. Al Zoubi Bahaa Al-Trad Mohamed M. Nasef Saurabh Satija Meenu Mehta Kavita Pabreja Vijay Mishra Mohammed Khan Salem Abobaker Ibrahim M. Azzouz Harish Dureja Ritesh M. Pabari Ashref Ali K. Dardouri Prashant Kesharwani Gaurav Gupta Shakti Dhar Shukla Parteek Prasher Nitin B. Charbe Poonam Negi Deepak N. Kapoor Dinesh Kumar Chellappan Mateus Webba da Silva Paul Thompson Kamal Dua Paul McCarron Murtaza M. Tambuwala Albumin Nano-Encapsulation of Piceatannol Enhances Its Anticancer Potential in Colon Cancer Via Downregulation of Nuclear p65 and HIF-1α Cancers piceatannol colon cancer albumin nanoparticles nuclear p65 hif-1α |
| title | Albumin Nano-Encapsulation of Piceatannol Enhances Its Anticancer Potential in Colon Cancer Via Downregulation of Nuclear p65 and HIF-1α |
| title_full | Albumin Nano-Encapsulation of Piceatannol Enhances Its Anticancer Potential in Colon Cancer Via Downregulation of Nuclear p65 and HIF-1α |
| title_fullStr | Albumin Nano-Encapsulation of Piceatannol Enhances Its Anticancer Potential in Colon Cancer Via Downregulation of Nuclear p65 and HIF-1α |
| title_full_unstemmed | Albumin Nano-Encapsulation of Piceatannol Enhances Its Anticancer Potential in Colon Cancer Via Downregulation of Nuclear p65 and HIF-1α |
| title_short | Albumin Nano-Encapsulation of Piceatannol Enhances Its Anticancer Potential in Colon Cancer Via Downregulation of Nuclear p65 and HIF-1α |
| title_sort | albumin nano encapsulation of piceatannol enhances its anticancer potential in colon cancer via downregulation of nuclear p65 and hif 1α |
| topic | piceatannol colon cancer albumin nanoparticles nuclear p65 hif-1α |
| url | https://www.mdpi.com/2072-6694/12/1/113 |
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