Generation of Virus- and dsRNA-Derived siRNAs with Species-Dependent Length in Insects
Double-stranded RNA (dsRNA) molecules of viral origin trigger a post-transcriptional gene-silencing mechanism called RNA interference (RNAi). Specifically, virally derived dsRNA is recognized and cleaved by the enzyme Dicer2 into short interfering RNAs (siRNAs), which further direct sequence-specifi...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-08-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/11/8/738 |
| _version_ | 1828174937603440640 |
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| author | Dulce Santos Lina Mingels Elise Vogel Luoluo Wang Olivier Christiaens Kaat Cappelle Niels Wynant Yannick Gansemans Filip Van Nieuwerburgh Guy Smagghe Luc Swevers Jozef Vanden Broeck |
| author_facet | Dulce Santos Lina Mingels Elise Vogel Luoluo Wang Olivier Christiaens Kaat Cappelle Niels Wynant Yannick Gansemans Filip Van Nieuwerburgh Guy Smagghe Luc Swevers Jozef Vanden Broeck |
| author_sort | Dulce Santos |
| collection | DOAJ |
| description | Double-stranded RNA (dsRNA) molecules of viral origin trigger a post-transcriptional gene-silencing mechanism called RNA interference (RNAi). Specifically, virally derived dsRNA is recognized and cleaved by the enzyme Dicer2 into short interfering RNAs (siRNAs), which further direct sequence-specific RNA silencing, ultimately silencing replication of the virus. Notably, RNAi can also be artificially triggered by the delivery of gene-specific dsRNA, thereby leading to endogenous gene silencing. This is a widely used technology that holds great potential to contribute to novel pest control strategies. In this regard, research efforts have been set to find methods to efficiently trigger RNAi in the field. In this article, we demonstrate the generation of dsRNA- and/or virus-derived siRNAs—the main RNAi effectors—in six insect species belonging to five economically important orders (Lepidoptera, Orthoptera, Hymenoptera, Coleoptera, and Diptera). In addition, we describe that the siRNA length distribution is species-dependent. Taken together, our results reveal interspecies variability in the (antiviral) RNAi mechanism in insects and show promise to contribute to future research on (viral-based) RNAi-triggering mechanisms in this class of animals. |
| first_indexed | 2024-04-12T04:20:01Z |
| format | Article |
| id | doaj.art-70d1626a4d80448e84699c8fa8b23e8e |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-04-12T04:20:01Z |
| publishDate | 2019-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-70d1626a4d80448e84699c8fa8b23e8e2022-12-22T03:48:15ZengMDPI AGViruses1999-49152019-08-0111873810.3390/v11080738v11080738Generation of Virus- and dsRNA-Derived siRNAs with Species-Dependent Length in InsectsDulce Santos0Lina Mingels1Elise Vogel2Luoluo Wang3Olivier Christiaens4Kaat Cappelle5Niels Wynant6Yannick Gansemans7Filip Van Nieuwerburgh8Guy Smagghe9Luc Swevers10Jozef Vanden Broeck11Research Group of Molecular Developmental Physiology and Signal Transduction, KU Leuven, 3000 Leuven, BelgiumResearch Group of Molecular Developmental Physiology and Signal Transduction, KU Leuven, 3000 Leuven, BelgiumResearch Group of Molecular Developmental Physiology and Signal Transduction, KU Leuven, 3000 Leuven, BelgiumLaboratory of Agrozoology, Department of Plants and Crops, Faculty of Bioscience Engineering, Ghent University, 9000 Ghent, BelgiumLaboratory of Agrozoology, Department of Plants and Crops, Faculty of Bioscience Engineering, Ghent University, 9000 Ghent, BelgiumLaboratory of Agrozoology, Department of Plants and Crops, Faculty of Bioscience Engineering, Ghent University, 9000 Ghent, BelgiumResearch Group of Molecular Developmental Physiology and Signal Transduction, KU Leuven, 3000 Leuven, BelgiumLaboratory of Pharmaceutical Biotechnology, Faculty of Pharmaceutical Sciences, Ghent University, 9000 Ghent, BelgiumLaboratory of Pharmaceutical Biotechnology, Faculty of Pharmaceutical Sciences, Ghent University, 9000 Ghent, BelgiumLaboratory of Agrozoology, Department of Plants and Crops, Faculty of Bioscience Engineering, Ghent University, 9000 Ghent, BelgiumInsect Molecular Genetics and Biotechnology Group, Institute of Biosciences and Applications, National Center for Scientific Research “Demokritos”, 15310 Athens, GreeceResearch Group of Molecular Developmental Physiology and Signal Transduction, KU Leuven, 3000 Leuven, BelgiumDouble-stranded RNA (dsRNA) molecules of viral origin trigger a post-transcriptional gene-silencing mechanism called RNA interference (RNAi). Specifically, virally derived dsRNA is recognized and cleaved by the enzyme Dicer2 into short interfering RNAs (siRNAs), which further direct sequence-specific RNA silencing, ultimately silencing replication of the virus. Notably, RNAi can also be artificially triggered by the delivery of gene-specific dsRNA, thereby leading to endogenous gene silencing. This is a widely used technology that holds great potential to contribute to novel pest control strategies. In this regard, research efforts have been set to find methods to efficiently trigger RNAi in the field. In this article, we demonstrate the generation of dsRNA- and/or virus-derived siRNAs—the main RNAi effectors—in six insect species belonging to five economically important orders (Lepidoptera, Orthoptera, Hymenoptera, Coleoptera, and Diptera). In addition, we describe that the siRNA length distribution is species-dependent. Taken together, our results reveal interspecies variability in the (antiviral) RNAi mechanism in insects and show promise to contribute to future research on (viral-based) RNAi-triggering mechanisms in this class of animals.https://www.mdpi.com/1999-4915/11/8/738insectsRNA interferencesmall interfering RNAssmall interfering RNA lengthdicerpest controlvirusesCrPVFHVdsRNA |
| spellingShingle | Dulce Santos Lina Mingels Elise Vogel Luoluo Wang Olivier Christiaens Kaat Cappelle Niels Wynant Yannick Gansemans Filip Van Nieuwerburgh Guy Smagghe Luc Swevers Jozef Vanden Broeck Generation of Virus- and dsRNA-Derived siRNAs with Species-Dependent Length in Insects Viruses insects RNA interference small interfering RNAs small interfering RNA length dicer pest control viruses CrPV FHV dsRNA |
| title | Generation of Virus- and dsRNA-Derived siRNAs with Species-Dependent Length in Insects |
| title_full | Generation of Virus- and dsRNA-Derived siRNAs with Species-Dependent Length in Insects |
| title_fullStr | Generation of Virus- and dsRNA-Derived siRNAs with Species-Dependent Length in Insects |
| title_full_unstemmed | Generation of Virus- and dsRNA-Derived siRNAs with Species-Dependent Length in Insects |
| title_short | Generation of Virus- and dsRNA-Derived siRNAs with Species-Dependent Length in Insects |
| title_sort | generation of virus and dsrna derived sirnas with species dependent length in insects |
| topic | insects RNA interference small interfering RNAs small interfering RNA length dicer pest control viruses CrPV FHV dsRNA |
| url | https://www.mdpi.com/1999-4915/11/8/738 |
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