Oxidative stress and toxicity produced by arsenic and chromium in broiler chicks and application of vitamin E and bentonite as ameliorating agents
The present study investigated the adverse effects of arsenic and chromium in broilers and ascertained the role of vitamin E and bentonite in alleviating their harmful effects. For this purpose, we experimented on 180 one-day-old broiler chickens. The feed was administered to broiler chicks of group...
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| Format: | Article |
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Frontiers Media S.A.
2023-03-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2023.1128522/full |
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| author | Javaria Mashkoor Fatimah A. Al-Saeed Zhang Guangbin Abdullah F. Alsayeqh Shafia Tehseen Gul Riaz Hussain Latif Ahmad Riaz Mustafa Umar Farooq Ahrar Khan Ahrar Khan |
| author_facet | Javaria Mashkoor Fatimah A. Al-Saeed Zhang Guangbin Abdullah F. Alsayeqh Shafia Tehseen Gul Riaz Hussain Latif Ahmad Riaz Mustafa Umar Farooq Ahrar Khan Ahrar Khan |
| author_sort | Javaria Mashkoor |
| collection | DOAJ |
| description | The present study investigated the adverse effects of arsenic and chromium in broilers and ascertained the role of vitamin E and bentonite in alleviating their harmful effects. For this purpose, we experimented on 180 one-day-old broiler chickens. The feed was administered to broiler chicks of groups 2, 6, 7, 8, and 9 chromium @ (270 mg.kg−1 BW). Groups 3, 6, 7, 8, and 9 were administered arsenic @ (50 mg.kg−1 BW). Groups 4, 7, and 9 received vitamin E (150 mg.kg−1 BW), and groups 5, 8, and 9 received bentonite (5%), respectively. Group 1 was kept in control. All the broiler chicks treated with chromium and arsenic showed a significant (p < 0.05) decline in erythrocytic parameters on experimental days 21 and 42. Total proteins decreased significantly, while ALT, AST, urea, and creatinine increased significantly (p < 0.05). TAC and CAT decreased significantly (p < 0.05), while TOC and MDA concentrations increased significantly (p < 0.05) in chromium and arsenic-treated groups on experimental days 21 and 42. Pearson correlation analysis revealed a strong positive correlation between TAC and CAT (Pearson correlation value = 0.961; p < 0.001), similarly TOC and MDA positive correlation (Pearson correlation value = 0.920; p < 0.001). However, TAC and CAT showed a negative correlation between TOC and MDA. The intensity of gross and microscopic lesions was more in chromium (270 mg.kg−1) and arsenic (50 mg.kg−1) singly or in combination-treated groups. Thus, broiler chicks treated with chromium plus arsenic exhibited higher gross and microscopic lesion intensity than other treated groups. Fatty degeneration, severe cytoplasmic vacuolar degeneration, and expansion of sinusoidal spaces were the main lesions observed in the liver. Kidneys showed renal epithelial cells necrosis, glomerular shrinkage, and severe cytoplasmic vacuolar degeneration. Co-administration of bentonite along with chromium and arsenic resulted in partial amelioration (group 8) compared to groups 7 and 9, administered arsenic + chromium + vitamin E and arsenic + chromium + vitamin E + bentonite, respectively. It was concluded that arsenic and chromium cause damage not only to haemato-biochemical parameters but also lead to oxidation stress in broilers. Vitamin E and bentonite administration can ameliorate toxicity and oxidative stress produced by arsenic and chromium. |
| first_indexed | 2024-04-10T05:23:42Z |
| format | Article |
| id | doaj.art-70d59f5a9928426d9eb8d203ee0f4d44 |
| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-04-10T05:23:42Z |
| publishDate | 2023-03-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Veterinary Science |
| spelling | doaj.art-70d59f5a9928426d9eb8d203ee0f4d442023-03-08T05:28:11ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692023-03-011010.3389/fvets.2023.11285221128522Oxidative stress and toxicity produced by arsenic and chromium in broiler chicks and application of vitamin E and bentonite as ameliorating agentsJavaria Mashkoor0Fatimah A. Al-Saeed1Zhang Guangbin2Abdullah F. Alsayeqh3Shafia Tehseen Gul4Riaz Hussain5Latif Ahmad6Riaz Mustafa7Umar Farooq8Ahrar Khan9Ahrar Khan10Department of Pathology, Faculty of Veterinary Science, University of Agriculture, Faisalabad, PakistanDepartment of Biology, College of Science, King Khalid University, Abha, Saudi ArabiaShandong Vocational Animal Science and Veterinary College, Weifang, ChinaDepartment of Veterinary Medicine, College of Agriculture and Veterinary Medicine, Qassim University, Buraidah, Qassim, Saudi ArabiaDepartment of Pathology, Faculty of Veterinary Science, University of Agriculture, Faisalabad, PakistanDepartment of Pathology, Faculty of Veterinary and Animal Sciences, The Islamia University of Bahawalpur, Bahawalpur, PakistanDepartment of Pre-clinical Studies, Faculty of Veterinary Medicine, Baqai Medical University, Karachi, PakistanUniversity of Agriculture, Faisalabad Sub Campus, Toba Tek Singh, PakistanUniversity of Agriculture, Faisalabad Sub Campus, Toba Tek Singh, PakistanDepartment of Pathology, Faculty of Veterinary Science, University of Agriculture, Faisalabad, PakistanShandong Vocational Animal Science and Veterinary College, Weifang, ChinaThe present study investigated the adverse effects of arsenic and chromium in broilers and ascertained the role of vitamin E and bentonite in alleviating their harmful effects. For this purpose, we experimented on 180 one-day-old broiler chickens. The feed was administered to broiler chicks of groups 2, 6, 7, 8, and 9 chromium @ (270 mg.kg−1 BW). Groups 3, 6, 7, 8, and 9 were administered arsenic @ (50 mg.kg−1 BW). Groups 4, 7, and 9 received vitamin E (150 mg.kg−1 BW), and groups 5, 8, and 9 received bentonite (5%), respectively. Group 1 was kept in control. All the broiler chicks treated with chromium and arsenic showed a significant (p < 0.05) decline in erythrocytic parameters on experimental days 21 and 42. Total proteins decreased significantly, while ALT, AST, urea, and creatinine increased significantly (p < 0.05). TAC and CAT decreased significantly (p < 0.05), while TOC and MDA concentrations increased significantly (p < 0.05) in chromium and arsenic-treated groups on experimental days 21 and 42. Pearson correlation analysis revealed a strong positive correlation between TAC and CAT (Pearson correlation value = 0.961; p < 0.001), similarly TOC and MDA positive correlation (Pearson correlation value = 0.920; p < 0.001). However, TAC and CAT showed a negative correlation between TOC and MDA. The intensity of gross and microscopic lesions was more in chromium (270 mg.kg−1) and arsenic (50 mg.kg−1) singly or in combination-treated groups. Thus, broiler chicks treated with chromium plus arsenic exhibited higher gross and microscopic lesion intensity than other treated groups. Fatty degeneration, severe cytoplasmic vacuolar degeneration, and expansion of sinusoidal spaces were the main lesions observed in the liver. Kidneys showed renal epithelial cells necrosis, glomerular shrinkage, and severe cytoplasmic vacuolar degeneration. Co-administration of bentonite along with chromium and arsenic resulted in partial amelioration (group 8) compared to groups 7 and 9, administered arsenic + chromium + vitamin E and arsenic + chromium + vitamin E + bentonite, respectively. It was concluded that arsenic and chromium cause damage not only to haemato-biochemical parameters but also lead to oxidation stress in broilers. Vitamin E and bentonite administration can ameliorate toxicity and oxidative stress produced by arsenic and chromium.https://www.frontiersin.org/articles/10.3389/fvets.2023.1128522/fullbroiler chicksarsenicchromiumtoxicityoxidative stressvitamin E |
| spellingShingle | Javaria Mashkoor Fatimah A. Al-Saeed Zhang Guangbin Abdullah F. Alsayeqh Shafia Tehseen Gul Riaz Hussain Latif Ahmad Riaz Mustafa Umar Farooq Ahrar Khan Ahrar Khan Oxidative stress and toxicity produced by arsenic and chromium in broiler chicks and application of vitamin E and bentonite as ameliorating agents Frontiers in Veterinary Science broiler chicks arsenic chromium toxicity oxidative stress vitamin E |
| title | Oxidative stress and toxicity produced by arsenic and chromium in broiler chicks and application of vitamin E and bentonite as ameliorating agents |
| title_full | Oxidative stress and toxicity produced by arsenic and chromium in broiler chicks and application of vitamin E and bentonite as ameliorating agents |
| title_fullStr | Oxidative stress and toxicity produced by arsenic and chromium in broiler chicks and application of vitamin E and bentonite as ameliorating agents |
| title_full_unstemmed | Oxidative stress and toxicity produced by arsenic and chromium in broiler chicks and application of vitamin E and bentonite as ameliorating agents |
| title_short | Oxidative stress and toxicity produced by arsenic and chromium in broiler chicks and application of vitamin E and bentonite as ameliorating agents |
| title_sort | oxidative stress and toxicity produced by arsenic and chromium in broiler chicks and application of vitamin e and bentonite as ameliorating agents |
| topic | broiler chicks arsenic chromium toxicity oxidative stress vitamin E |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2023.1128522/full |
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