Role of the postinspiratory complex in regulating swallow–breathing coordination and other laryngeal behaviors
Breathing needs to be tightly coordinated with upper airway behaviors, such as swallowing. Discoordination leads to aspiration pneumonia, the leading cause of death in neurodegenerative disease. Here, we study the role of the postinspiratory complex (PiCo) in coordinating breathing and swallowing. U...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2023-06-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/86103 |
| _version_ | 1797798207591284736 |
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| author | Alyssa Huff Marlusa Karlen-Amarante Luiz M Oliveira Jan-Marino Ramirez |
| author_facet | Alyssa Huff Marlusa Karlen-Amarante Luiz M Oliveira Jan-Marino Ramirez |
| author_sort | Alyssa Huff |
| collection | DOAJ |
| description | Breathing needs to be tightly coordinated with upper airway behaviors, such as swallowing. Discoordination leads to aspiration pneumonia, the leading cause of death in neurodegenerative disease. Here, we study the role of the postinspiratory complex (PiCo) in coordinating breathing and swallowing. Using optogenetic approaches in freely breathing anesthetized ChATcre:Ai32, Vglut2cre:Ai32 and intersectional recombination of ChATcre:Vglut2FlpO:ChR2 mice reveals PiCo mediates airway protective behaviors. Activation of PiCo during inspiration or the beginning of postinspiration triggers swallow behavior in an all-or-nothing manner, while there is a higher probability for stimulating only laryngeal activation when activated further into expiration. Laryngeal activation is dependent on stimulation duration. Sufficient bilateral PiCo activation is necessary for preserving the physiological swallow motor sequence since activation of only a few PiCo neurons or unilateral activation leads to blurred upper airway behavioral responses. We believe PiCo acts as an interface between the swallow pattern generator and the preBötzinger complex to coordinate swallow and breathing. Investigating PiCo’s role in swallow and laryngeal coordination will aid in understanding discoordination with breathing in neurological diseases. |
| first_indexed | 2024-03-13T04:00:00Z |
| format | Article |
| id | doaj.art-70d677efce2f4a93b430960f9100f78e |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-03-13T04:00:00Z |
| publishDate | 2023-06-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-70d677efce2f4a93b430960f9100f78e2023-06-21T15:25:28ZengeLife Sciences Publications LtdeLife2050-084X2023-06-011210.7554/eLife.86103Role of the postinspiratory complex in regulating swallow–breathing coordination and other laryngeal behaviorsAlyssa Huff0https://orcid.org/0000-0003-2817-251XMarlusa Karlen-Amarante1Luiz M Oliveira2Jan-Marino Ramirez3https://orcid.org/0000-0002-5626-3999Center for Integrative Brain Research, Seattle Children’s Research Institute, Seattle, United StatesCenter for Integrative Brain Research, Seattle Children’s Research Institute, Seattle, United StatesCenter for Integrative Brain Research, Seattle Children’s Research Institute, Seattle, United StatesCenter for Integrative Brain Research, Seattle Children’s Research Institute, Seattle, United States; Department of Neurological Surgery, University of Washington School of Medicine, Seattle, United StatesBreathing needs to be tightly coordinated with upper airway behaviors, such as swallowing. Discoordination leads to aspiration pneumonia, the leading cause of death in neurodegenerative disease. Here, we study the role of the postinspiratory complex (PiCo) in coordinating breathing and swallowing. Using optogenetic approaches in freely breathing anesthetized ChATcre:Ai32, Vglut2cre:Ai32 and intersectional recombination of ChATcre:Vglut2FlpO:ChR2 mice reveals PiCo mediates airway protective behaviors. Activation of PiCo during inspiration or the beginning of postinspiration triggers swallow behavior in an all-or-nothing manner, while there is a higher probability for stimulating only laryngeal activation when activated further into expiration. Laryngeal activation is dependent on stimulation duration. Sufficient bilateral PiCo activation is necessary for preserving the physiological swallow motor sequence since activation of only a few PiCo neurons or unilateral activation leads to blurred upper airway behavioral responses. We believe PiCo acts as an interface between the swallow pattern generator and the preBötzinger complex to coordinate swallow and breathing. Investigating PiCo’s role in swallow and laryngeal coordination will aid in understanding discoordination with breathing in neurological diseases.https://elifesciences.org/articles/86103control of breathingswallow functionneurocircuitry |
| spellingShingle | Alyssa Huff Marlusa Karlen-Amarante Luiz M Oliveira Jan-Marino Ramirez Role of the postinspiratory complex in regulating swallow–breathing coordination and other laryngeal behaviors eLife control of breathing swallow function neurocircuitry |
| title | Role of the postinspiratory complex in regulating swallow–breathing coordination and other laryngeal behaviors |
| title_full | Role of the postinspiratory complex in regulating swallow–breathing coordination and other laryngeal behaviors |
| title_fullStr | Role of the postinspiratory complex in regulating swallow–breathing coordination and other laryngeal behaviors |
| title_full_unstemmed | Role of the postinspiratory complex in regulating swallow–breathing coordination and other laryngeal behaviors |
| title_short | Role of the postinspiratory complex in regulating swallow–breathing coordination and other laryngeal behaviors |
| title_sort | role of the postinspiratory complex in regulating swallow breathing coordination and other laryngeal behaviors |
| topic | control of breathing swallow function neurocircuitry |
| url | https://elifesciences.org/articles/86103 |
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