Targeted proteomic analysis dataset of archival core human kidney biopsies to investigate the biology of hypertensive nephropathy
Hypertensive nephropathy is the second most frequent cause of end-stage renal disease in western societies. In previous experiments in our laboratory with proteomic analysis of renal parenchyma of SHR hypertensive animals, we identified two molecules, namely SGLT2 and CLIC4, associated with the deve...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-02-01
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| Series: | Data in Brief |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352340922000178 |
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| author | Georgios Barkas Manousos Makridakis Hariklia Gakiopoulou Demetrios Vlahakos |
| author_facet | Georgios Barkas Manousos Makridakis Hariklia Gakiopoulou Demetrios Vlahakos |
| author_sort | Georgios Barkas |
| collection | DOAJ |
| description | Hypertensive nephropathy is the second most frequent cause of end-stage renal disease in western societies. In previous experiments in our laboratory with proteomic analysis of renal parenchyma of SHR hypertensive animals, we identified two molecules, namely SGLT2 and CLIC4, associated with the development of hypertension. Here, we apply the methodology of targeted proteomic analysis in kidney biopsies from patients with hypertensive nephropathy to study the role of SGLT2 and CLIC4 in the pathogenesis of the disease. Relative quantification data of SGLT2 and CLIC4 via means of targeted proteomic analysis in human kidney biopsies from hypertensive patients and normotensive controls are reported. In addition, validation data of the proteomic results via immunofluorescence are presented. Renal tissue biopsies (N = 17) from archival material of patients with hypertensive nephropathy and normotensive controls were used. Targeted proteomic analysis was performed using the method: ``Parallel Reaction Monitoring'' (PRM) in renal parenchyma of hypertensive and normotensive patients for the selective identification of SGLT2 and CLIC4 and the relative quantification of their expression using proteotypic peptides for each protein. The expression of SGLT2 molecule was also confirmed by immunofluorescence followed by quantification of fluorescence intensity. According to PRM, the SGLT2 protein was found with reduced and the CLIC4 protein with increased expression levels in hypertensive patients compared to normotensive controls. Comparison of representative immunofluorescence images confirmed a decrease in the expression of SGLT2 in the brush border of proximal tubular epithelial cells in hypertensive patients. Our data show changes in the tubular compartment of the kidney and especially in the proximal tubules associated with the hypertensive nephropathy. The clinical significance of these findings should be further explored for the discovery and/or confirmation of novel therapeutic approaches and biomarkers in the development of hypertensive kidney disease. |
| first_indexed | 2024-12-24T00:22:54Z |
| format | Article |
| id | doaj.art-70d82ecf1fd947188f7fe2d9b1dcb327 |
| institution | Directory Open Access Journal |
| issn | 2352-3409 |
| language | English |
| last_indexed | 2024-12-24T00:22:54Z |
| publishDate | 2022-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Data in Brief |
| spelling | doaj.art-70d82ecf1fd947188f7fe2d9b1dcb3272022-12-21T17:24:32ZengElsevierData in Brief2352-34092022-02-0140107805Targeted proteomic analysis dataset of archival core human kidney biopsies to investigate the biology of hypertensive nephropathyGeorgios Barkas0Manousos Makridakis1Hariklia Gakiopoulou2Demetrios Vlahakos3Biomedical Research Foundation of the Academy of Athens, GreeceBiomedical Research Foundation of the Academy of Athens, Greece; Corresponding author.National and Kapodistrian University of Athens Medical School, Athens, GreeceNational and Kapodistrian University of Athens Medical School, Athens, GreeceHypertensive nephropathy is the second most frequent cause of end-stage renal disease in western societies. In previous experiments in our laboratory with proteomic analysis of renal parenchyma of SHR hypertensive animals, we identified two molecules, namely SGLT2 and CLIC4, associated with the development of hypertension. Here, we apply the methodology of targeted proteomic analysis in kidney biopsies from patients with hypertensive nephropathy to study the role of SGLT2 and CLIC4 in the pathogenesis of the disease. Relative quantification data of SGLT2 and CLIC4 via means of targeted proteomic analysis in human kidney biopsies from hypertensive patients and normotensive controls are reported. In addition, validation data of the proteomic results via immunofluorescence are presented. Renal tissue biopsies (N = 17) from archival material of patients with hypertensive nephropathy and normotensive controls were used. Targeted proteomic analysis was performed using the method: ``Parallel Reaction Monitoring'' (PRM) in renal parenchyma of hypertensive and normotensive patients for the selective identification of SGLT2 and CLIC4 and the relative quantification of their expression using proteotypic peptides for each protein. The expression of SGLT2 molecule was also confirmed by immunofluorescence followed by quantification of fluorescence intensity. According to PRM, the SGLT2 protein was found with reduced and the CLIC4 protein with increased expression levels in hypertensive patients compared to normotensive controls. Comparison of representative immunofluorescence images confirmed a decrease in the expression of SGLT2 in the brush border of proximal tubular epithelial cells in hypertensive patients. Our data show changes in the tubular compartment of the kidney and especially in the proximal tubules associated with the hypertensive nephropathy. The clinical significance of these findings should be further explored for the discovery and/or confirmation of novel therapeutic approaches and biomarkers in the development of hypertensive kidney disease.http://www.sciencedirect.com/science/article/pii/S2352340922000178Parallel Reaction Monitoring (PRM)ProteomicsImmunofluorescenceHypertensive nephropathyHuman Kidney BiopsiesSGLT2 |
| spellingShingle | Georgios Barkas Manousos Makridakis Hariklia Gakiopoulou Demetrios Vlahakos Targeted proteomic analysis dataset of archival core human kidney biopsies to investigate the biology of hypertensive nephropathy Data in Brief Parallel Reaction Monitoring (PRM) Proteomics Immunofluorescence Hypertensive nephropathy Human Kidney Biopsies SGLT2 |
| title | Targeted proteomic analysis dataset of archival core human kidney biopsies to investigate the biology of hypertensive nephropathy |
| title_full | Targeted proteomic analysis dataset of archival core human kidney biopsies to investigate the biology of hypertensive nephropathy |
| title_fullStr | Targeted proteomic analysis dataset of archival core human kidney biopsies to investigate the biology of hypertensive nephropathy |
| title_full_unstemmed | Targeted proteomic analysis dataset of archival core human kidney biopsies to investigate the biology of hypertensive nephropathy |
| title_short | Targeted proteomic analysis dataset of archival core human kidney biopsies to investigate the biology of hypertensive nephropathy |
| title_sort | targeted proteomic analysis dataset of archival core human kidney biopsies to investigate the biology of hypertensive nephropathy |
| topic | Parallel Reaction Monitoring (PRM) Proteomics Immunofluorescence Hypertensive nephropathy Human Kidney Biopsies SGLT2 |
| url | http://www.sciencedirect.com/science/article/pii/S2352340922000178 |
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