<i>OTUD7A</i> Regulates Inflammation- and Immune-Related Gene Expression in Goose Fatty Liver
OTU deubiquitinase 7A (<i>OTUD7A</i>) can suppress inflammation signaling pathways, but it is unclear whether the gene can inhibit inflammation in goose fatty liver. In order to investigate the functions of <i>OTUD7A</i> and identify the genes and pathways subjected to the re...
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| Format: | Article |
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MDPI AG
2022-01-01
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| Series: | Agriculture |
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| Online Access: | https://www.mdpi.com/2077-0472/12/1/105 |
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| author | Minmeng Zhao Kang Wen Xiang Fan Qingyun Sun Diego Jauregui Mawahib K. Khogali Long Liu Tuoyu Geng Daoqing Gong |
| author_facet | Minmeng Zhao Kang Wen Xiang Fan Qingyun Sun Diego Jauregui Mawahib K. Khogali Long Liu Tuoyu Geng Daoqing Gong |
| author_sort | Minmeng Zhao |
| collection | DOAJ |
| description | OTU deubiquitinase 7A (<i>OTUD7A</i>) can suppress inflammation signaling pathways, but it is unclear whether the gene can inhibit inflammation in goose fatty liver. In order to investigate the functions of <i>OTUD7A</i> and identify the genes and pathways subjected to the regulation of OTUD7A in the formation of goose fatty liver, we conducted transcriptomic analysis of cells, which revealed several genes related to inflammation and immunity that were significantly differentially expressed after <i>OTUD7A</i> overexpression. Moreover, the expression of interferon-induced protein with tetratricopeptide repeats 5 (<i>IFIT5</i>), tumor necrosis factor ligand superfamily member 8 (<i>TNFSF8</i>), sterile alpha motif domain-containing protein 9 (<i>SAMD9</i>), radical <i>S</i>-adenosyl methionine domain-containing protein 2 (<i>RSAD2</i>), interferon-induced GTP-binding protein Mx1 (<i>MX1</i>), and interferon-induced guanylate binding protein 1-like (<i>GBP1</i>) was inhibited by <i>OTUD7A</i> overexpression but induced by <i>OTUD7A</i> knockdown with small interfering RNA in goose hepatocytes. Furthermore, the mRNA expression of <i>IFIT5</i>, <i>TNFSF8</i>, <i>SAMD9</i>, <i>RSAD2</i>, <i>MX1</i>, and <i>GBP1</i> was downregulated, whereas <i>OTUD7A</i> expression was upregulated in goose fatty liver after 12 days of overfeeding. In contrast, the expression patterns of these genes showed nearly the opposite trend after 24 days of overfeeding. Taken together, these findings indicate that <i>OTUD7A</i> regulates the expression of inflammation- and immune-related genes in the development of goose fatty liver. |
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| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2077-0472 |
| language | English |
| last_indexed | 2024-03-10T03:08:35Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agriculture |
| spelling | doaj.art-70da5cc56e3d43e6a948a9c1b3bb713d2023-11-23T12:36:18ZengMDPI AGAgriculture2077-04722022-01-0112110510.3390/agriculture12010105<i>OTUD7A</i> Regulates Inflammation- and Immune-Related Gene Expression in Goose Fatty LiverMinmeng Zhao0Kang Wen1Xiang Fan2Qingyun Sun3Diego Jauregui4Mawahib K. Khogali5Long Liu6Tuoyu Geng7Daoqing Gong8College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaCollege of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaCollege of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaCollege of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaCollege of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaCollege of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaCollege of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaCollege of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaCollege of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaOTU deubiquitinase 7A (<i>OTUD7A</i>) can suppress inflammation signaling pathways, but it is unclear whether the gene can inhibit inflammation in goose fatty liver. In order to investigate the functions of <i>OTUD7A</i> and identify the genes and pathways subjected to the regulation of OTUD7A in the formation of goose fatty liver, we conducted transcriptomic analysis of cells, which revealed several genes related to inflammation and immunity that were significantly differentially expressed after <i>OTUD7A</i> overexpression. Moreover, the expression of interferon-induced protein with tetratricopeptide repeats 5 (<i>IFIT5</i>), tumor necrosis factor ligand superfamily member 8 (<i>TNFSF8</i>), sterile alpha motif domain-containing protein 9 (<i>SAMD9</i>), radical <i>S</i>-adenosyl methionine domain-containing protein 2 (<i>RSAD2</i>), interferon-induced GTP-binding protein Mx1 (<i>MX1</i>), and interferon-induced guanylate binding protein 1-like (<i>GBP1</i>) was inhibited by <i>OTUD7A</i> overexpression but induced by <i>OTUD7A</i> knockdown with small interfering RNA in goose hepatocytes. Furthermore, the mRNA expression of <i>IFIT5</i>, <i>TNFSF8</i>, <i>SAMD9</i>, <i>RSAD2</i>, <i>MX1</i>, and <i>GBP1</i> was downregulated, whereas <i>OTUD7A</i> expression was upregulated in goose fatty liver after 12 days of overfeeding. In contrast, the expression patterns of these genes showed nearly the opposite trend after 24 days of overfeeding. Taken together, these findings indicate that <i>OTUD7A</i> regulates the expression of inflammation- and immune-related genes in the development of goose fatty liver.https://www.mdpi.com/2077-0472/12/1/105<i>OTUD7A</i>gooseinflammationimmunenonalcoholic fatty liver disease |
| spellingShingle | Minmeng Zhao Kang Wen Xiang Fan Qingyun Sun Diego Jauregui Mawahib K. Khogali Long Liu Tuoyu Geng Daoqing Gong <i>OTUD7A</i> Regulates Inflammation- and Immune-Related Gene Expression in Goose Fatty Liver Agriculture <i>OTUD7A</i> goose inflammation immune nonalcoholic fatty liver disease |
| title | <i>OTUD7A</i> Regulates Inflammation- and Immune-Related Gene Expression in Goose Fatty Liver |
| title_full | <i>OTUD7A</i> Regulates Inflammation- and Immune-Related Gene Expression in Goose Fatty Liver |
| title_fullStr | <i>OTUD7A</i> Regulates Inflammation- and Immune-Related Gene Expression in Goose Fatty Liver |
| title_full_unstemmed | <i>OTUD7A</i> Regulates Inflammation- and Immune-Related Gene Expression in Goose Fatty Liver |
| title_short | <i>OTUD7A</i> Regulates Inflammation- and Immune-Related Gene Expression in Goose Fatty Liver |
| title_sort | i otud7a i regulates inflammation and immune related gene expression in goose fatty liver |
| topic | <i>OTUD7A</i> goose inflammation immune nonalcoholic fatty liver disease |
| url | https://www.mdpi.com/2077-0472/12/1/105 |
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