The characterization of modified starch branching enzymes: toward the control of starch chain-length distributions.
Starch is a complex branched glucose polymer whose branch molecular weight distribution (the chain-length distribution, CLD) influences nutritionally important properties such as digestion rate. Chain-stopping in starch biosynthesis is by starch branching enzyme (SBE). Site-directed mutagenesis was...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4395411?pdf=render |
| _version_ | 1811207863691902976 |
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| author | Cheng Li Alex Chi Wu Rob Marc Go Jacob Malouf Mark S Turner Alpeshkumar K Malde Alan E Mark Robert G Gilbert |
| author_facet | Cheng Li Alex Chi Wu Rob Marc Go Jacob Malouf Mark S Turner Alpeshkumar K Malde Alan E Mark Robert G Gilbert |
| author_sort | Cheng Li |
| collection | DOAJ |
| description | Starch is a complex branched glucose polymer whose branch molecular weight distribution (the chain-length distribution, CLD) influences nutritionally important properties such as digestion rate. Chain-stopping in starch biosynthesis is by starch branching enzyme (SBE). Site-directed mutagenesis was used to modify SBEIIa from Zea mays (mSBEIIa) to produce mutants, each differing in a single conserved amino-acid residue. Products at different times from in vitro branching were debranched and the time evolution of the CLD measured by size-exclusion chromatography. The results confirm that Tyr352, Glu513, and Ser349 are important for mSBEIIa activity while Arg456 is important for determining the position at which the linear glucan is cut. The mutant mSBEIIa enzymes have different activities and suggest the length of the transferred chain can be varied by mutation. The work shows analysis of the molecular weight distribution can yield information regarding the enzyme branching sites useful for development of plants yielding starch with improved functionality. |
| first_indexed | 2024-04-12T04:12:01Z |
| format | Article |
| id | doaj.art-70db23f0bdcc4bbeabab818d5ea4f3d5 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T04:12:01Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-70db23f0bdcc4bbeabab818d5ea4f3d52022-12-22T03:48:29ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01104e012550710.1371/journal.pone.0125507The characterization of modified starch branching enzymes: toward the control of starch chain-length distributions.Cheng LiAlex Chi WuRob Marc GoJacob MaloufMark S TurnerAlpeshkumar K MaldeAlan E MarkRobert G GilbertStarch is a complex branched glucose polymer whose branch molecular weight distribution (the chain-length distribution, CLD) influences nutritionally important properties such as digestion rate. Chain-stopping in starch biosynthesis is by starch branching enzyme (SBE). Site-directed mutagenesis was used to modify SBEIIa from Zea mays (mSBEIIa) to produce mutants, each differing in a single conserved amino-acid residue. Products at different times from in vitro branching were debranched and the time evolution of the CLD measured by size-exclusion chromatography. The results confirm that Tyr352, Glu513, and Ser349 are important for mSBEIIa activity while Arg456 is important for determining the position at which the linear glucan is cut. The mutant mSBEIIa enzymes have different activities and suggest the length of the transferred chain can be varied by mutation. The work shows analysis of the molecular weight distribution can yield information regarding the enzyme branching sites useful for development of plants yielding starch with improved functionality.http://europepmc.org/articles/PMC4395411?pdf=render |
| spellingShingle | Cheng Li Alex Chi Wu Rob Marc Go Jacob Malouf Mark S Turner Alpeshkumar K Malde Alan E Mark Robert G Gilbert The characterization of modified starch branching enzymes: toward the control of starch chain-length distributions. PLoS ONE |
| title | The characterization of modified starch branching enzymes: toward the control of starch chain-length distributions. |
| title_full | The characterization of modified starch branching enzymes: toward the control of starch chain-length distributions. |
| title_fullStr | The characterization of modified starch branching enzymes: toward the control of starch chain-length distributions. |
| title_full_unstemmed | The characterization of modified starch branching enzymes: toward the control of starch chain-length distributions. |
| title_short | The characterization of modified starch branching enzymes: toward the control of starch chain-length distributions. |
| title_sort | characterization of modified starch branching enzymes toward the control of starch chain length distributions |
| url | http://europepmc.org/articles/PMC4395411?pdf=render |
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