Global transcriptome analysis of two <it>ameiotic1 </it>alleles in maize anthers: defining steps in meiotic entry and progression through prophase I
<p>Abstract</p> <p>Background</p> <p>Developmental cues to start meiosis occur late in plants. <it>Ameiotic1 </it>(<it>Am1</it>) encodes a plant-specific nuclear protein (AM1) required for meiotic entry and progression through early prophase I. P...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2011-08-01
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| Series: | BMC Plant Biology |
| Subjects: | |
| Online Access: | http://www.biomedcentral.com/1471-2229/11/120 |
| _version_ | 1811244024101601280 |
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| author | Fernandes John F Wang Rachel C Ronceret Arnaud Nan Guo-Ling Cande W Zacheus Walbot Virginia |
| author_facet | Fernandes John F Wang Rachel C Ronceret Arnaud Nan Guo-Ling Cande W Zacheus Walbot Virginia |
| author_sort | Fernandes John F |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Developmental cues to start meiosis occur late in plants. <it>Ameiotic1 </it>(<it>Am1</it>) encodes a plant-specific nuclear protein (AM1) required for meiotic entry and progression through early prophase I. Pollen mother cells (PMCs) remain mitotic in most <it>am1 </it>mutants including <it>am1-489</it>, while <it>am1-praI </it>permits meiotic entry but PMCs arrest at the leptotene/zygotene (L/Z) transition, defining the roles of AM1 protein in two distinct steps of meiosis. To gain more insights into the roles of AM1 in the transcriptional pre-meiotic and meiotic programs, we report here an in depth analysis of gene expression alterations in carefully staged anthers at 1 mm (meiotic entry) and 1.5 mm (L/Z) caused by each of these <it>am1 </it>alleles.</p> <p>Results</p> <p>1.0 mm and 1.5 mm anthers of <it>am1-489 </it>and <it>am1-praI </it>were profiled in comparison to fertile siblings on Agilent<sup>® </sup>4 × 44 K microarrays. Both <it>am1-489 </it>and <it>am1-praI </it>anthers are cytologically normal at 1.0 mm and show moderate transcriptome alterations. At the 1.5-mm stage both mutants are aberrant cytologically, and show more drastic transcriptome changes. There are substantially more absolute On/Off and twice as many differentially expressed genes (sterile versus fertile) in <it>am1-489 </it>than in <it>am1-praI</it>. At 1.5 mm a total of 4,418 genes are up- or down-regulated in either <it>am1-489 </it>or <it>am1-praI </it>anthers. These are predominantly stage-specific transcripts. Many putative meiosis-related genes were found among them including a small subset of allele-specific, mis-regulated genes specific to the PMCs. Nearly 60% of transcriptome changes in the set of transcripts mis-regulated in both mutants (N = 530) are enriched in PMCs, and only 1% are enriched in the tapetal cell transcriptome. All array data reported herein will be deposited and accessible at MaizeGDB <url>http://www.maizegdb.org/</url>.</p> <p>Conclusions</p> <p>Our analysis of anther transcriptome modulations by two distinct <it>am1 </it>alleles, <it>am1-489 </it>and <it>am1-praI</it>, redefines the role of AM1 as a modulator of expression of a subset of meiotic genes, important for meiotic progression and provided stage-specific insights into the genetic networks associated with meiotic entry and early prophase I progression.</p> |
| first_indexed | 2024-04-12T14:18:35Z |
| format | Article |
| id | doaj.art-70dbe85f11734fd3a68b1a644b43a2ff |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-04-12T14:18:35Z |
| publishDate | 2011-08-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-70dbe85f11734fd3a68b1a644b43a2ff2022-12-22T03:29:39ZengBMCBMC Plant Biology1471-22292011-08-0111112010.1186/1471-2229-11-120Global transcriptome analysis of two <it>ameiotic1 </it>alleles in maize anthers: defining steps in meiotic entry and progression through prophase IFernandes John FWang Rachel CRonceret ArnaudNan Guo-LingCande W ZacheusWalbot Virginia<p>Abstract</p> <p>Background</p> <p>Developmental cues to start meiosis occur late in plants. <it>Ameiotic1 </it>(<it>Am1</it>) encodes a plant-specific nuclear protein (AM1) required for meiotic entry and progression through early prophase I. Pollen mother cells (PMCs) remain mitotic in most <it>am1 </it>mutants including <it>am1-489</it>, while <it>am1-praI </it>permits meiotic entry but PMCs arrest at the leptotene/zygotene (L/Z) transition, defining the roles of AM1 protein in two distinct steps of meiosis. To gain more insights into the roles of AM1 in the transcriptional pre-meiotic and meiotic programs, we report here an in depth analysis of gene expression alterations in carefully staged anthers at 1 mm (meiotic entry) and 1.5 mm (L/Z) caused by each of these <it>am1 </it>alleles.</p> <p>Results</p> <p>1.0 mm and 1.5 mm anthers of <it>am1-489 </it>and <it>am1-praI </it>were profiled in comparison to fertile siblings on Agilent<sup>® </sup>4 × 44 K microarrays. Both <it>am1-489 </it>and <it>am1-praI </it>anthers are cytologically normal at 1.0 mm and show moderate transcriptome alterations. At the 1.5-mm stage both mutants are aberrant cytologically, and show more drastic transcriptome changes. There are substantially more absolute On/Off and twice as many differentially expressed genes (sterile versus fertile) in <it>am1-489 </it>than in <it>am1-praI</it>. At 1.5 mm a total of 4,418 genes are up- or down-regulated in either <it>am1-489 </it>or <it>am1-praI </it>anthers. These are predominantly stage-specific transcripts. Many putative meiosis-related genes were found among them including a small subset of allele-specific, mis-regulated genes specific to the PMCs. Nearly 60% of transcriptome changes in the set of transcripts mis-regulated in both mutants (N = 530) are enriched in PMCs, and only 1% are enriched in the tapetal cell transcriptome. All array data reported herein will be deposited and accessible at MaizeGDB <url>http://www.maizegdb.org/</url>.</p> <p>Conclusions</p> <p>Our analysis of anther transcriptome modulations by two distinct <it>am1 </it>alleles, <it>am1-489 </it>and <it>am1-praI</it>, redefines the role of AM1 as a modulator of expression of a subset of meiotic genes, important for meiotic progression and provided stage-specific insights into the genetic networks associated with meiotic entry and early prophase I progression.</p>http://www.biomedcentral.com/1471-2229/11/120meiosismeiocytestranscriptomesleptotene/zygotene transitionpollen mother cells |
| spellingShingle | Fernandes John F Wang Rachel C Ronceret Arnaud Nan Guo-Ling Cande W Zacheus Walbot Virginia Global transcriptome analysis of two <it>ameiotic1 </it>alleles in maize anthers: defining steps in meiotic entry and progression through prophase I BMC Plant Biology meiosis meiocytes transcriptomes leptotene/zygotene transition pollen mother cells |
| title | Global transcriptome analysis of two <it>ameiotic1 </it>alleles in maize anthers: defining steps in meiotic entry and progression through prophase I |
| title_full | Global transcriptome analysis of two <it>ameiotic1 </it>alleles in maize anthers: defining steps in meiotic entry and progression through prophase I |
| title_fullStr | Global transcriptome analysis of two <it>ameiotic1 </it>alleles in maize anthers: defining steps in meiotic entry and progression through prophase I |
| title_full_unstemmed | Global transcriptome analysis of two <it>ameiotic1 </it>alleles in maize anthers: defining steps in meiotic entry and progression through prophase I |
| title_short | Global transcriptome analysis of two <it>ameiotic1 </it>alleles in maize anthers: defining steps in meiotic entry and progression through prophase I |
| title_sort | global transcriptome analysis of two it ameiotic1 it alleles in maize anthers defining steps in meiotic entry and progression through prophase i |
| topic | meiosis meiocytes transcriptomes leptotene/zygotene transition pollen mother cells |
| url | http://www.biomedcentral.com/1471-2229/11/120 |
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