Detection of TRPM6 and TRPM7 Proteins in Normal and Diseased Cardiac Atrial Tissue and Isolated Cardiomyocytes
Magnesium-sensitive transient receptor potential melastatin (TRPM) ion channels, TRPM6 and TRPM7, are present in several organs, but their roles in the heart remain unclear. Therefore, here, we studied the expression patterns of TRPM6 and TRPM7 in normal and diseased myocardium. Cardiac atrial tissu...
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| Format: | Article |
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MDPI AG
2022-11-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/23/14860 |
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| author | Inga Andriulė Dalia Pangonytė Asfree Gwanyanya Dainius Karčiauskas Kanigula Mubagwa Regina Mačianskienė |
| author_facet | Inga Andriulė Dalia Pangonytė Asfree Gwanyanya Dainius Karčiauskas Kanigula Mubagwa Regina Mačianskienė |
| author_sort | Inga Andriulė |
| collection | DOAJ |
| description | Magnesium-sensitive transient receptor potential melastatin (TRPM) ion channels, TRPM6 and TRPM7, are present in several organs, but their roles in the heart remain unclear. Therefore, here, we studied the expression patterns of TRPM6 and TRPM7 in normal and diseased myocardium. Cardiac atrial tissue and cardiomyocytes were obtained from healthy pigs and undiseased human hearts as well as from hearts of patients with ischemic heart disease (IHD) or atrial fibrillation (AF). Immunofluorescence and ELISA were used to detect TRP proteins. TRPM6 and TRPM7 immunofluorescence signals, localized at/near the cell surface or intracellularly, were detected in pig and human atrial tissues. The TRP channel modulators carvacrol (CAR, 100 µM) or 2-aminoethoxydiphenyl borate (2-APB, 500 µM) decreased the TRPM7 signal, but enhanced that of TRPM6. At a higher concentration (2 mM), 2-APB enhanced the signals of both proteins. TRPM6 and TRPM7 immunofluorescence signals and protein concentrations were increased in atrial cells and tissues from IHD or AF patients. TRPM6 and TRPM7 proteins were both detected in cardiac atrial tissue, with relatively similar subcellular localization, but distinctive drug sensitivity profiles. Their upregulated expression in IHD and AF suggests a possible role of the channels in cardiac atrial disease. |
| first_indexed | 2024-03-09T17:46:31Z |
| format | Article |
| id | doaj.art-70de1fe0e8d6478da4850c293e21f252 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T17:46:31Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-70de1fe0e8d6478da4850c293e21f2522023-11-24T11:09:08ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-11-0123231486010.3390/ijms232314860Detection of TRPM6 and TRPM7 Proteins in Normal and Diseased Cardiac Atrial Tissue and Isolated CardiomyocytesInga Andriulė0Dalia Pangonytė1Asfree Gwanyanya2Dainius Karčiauskas3Kanigula Mubagwa4Regina Mačianskienė5Institute of Cardiology, Lithuanian University of Health Sciences, 50103 Kaunas, LithuaniaInstitute of Cardiology, Lithuanian University of Health Sciences, 50103 Kaunas, LithuaniaDepartment of Human Biology, Faculty of Health Sciences, University of Cape Town, Cape Town 7700, South AfricaInstitute of Cardiology, Lithuanian University of Health Sciences, 50103 Kaunas, LithuaniaDepartment of Cardiovascular Sciences, Faculty of Medicine, KU Leuven, 3000 Leuven, BelgiumInstitute of Cardiology, Lithuanian University of Health Sciences, 50103 Kaunas, LithuaniaMagnesium-sensitive transient receptor potential melastatin (TRPM) ion channels, TRPM6 and TRPM7, are present in several organs, but their roles in the heart remain unclear. Therefore, here, we studied the expression patterns of TRPM6 and TRPM7 in normal and diseased myocardium. Cardiac atrial tissue and cardiomyocytes were obtained from healthy pigs and undiseased human hearts as well as from hearts of patients with ischemic heart disease (IHD) or atrial fibrillation (AF). Immunofluorescence and ELISA were used to detect TRP proteins. TRPM6 and TRPM7 immunofluorescence signals, localized at/near the cell surface or intracellularly, were detected in pig and human atrial tissues. The TRP channel modulators carvacrol (CAR, 100 µM) or 2-aminoethoxydiphenyl borate (2-APB, 500 µM) decreased the TRPM7 signal, but enhanced that of TRPM6. At a higher concentration (2 mM), 2-APB enhanced the signals of both proteins. TRPM6 and TRPM7 immunofluorescence signals and protein concentrations were increased in atrial cells and tissues from IHD or AF patients. TRPM6 and TRPM7 proteins were both detected in cardiac atrial tissue, with relatively similar subcellular localization, but distinctive drug sensitivity profiles. Their upregulated expression in IHD and AF suggests a possible role of the channels in cardiac atrial disease.https://www.mdpi.com/1422-0067/23/23/14860TRPM6 and TRPM7 channelsatrial myocyte and tissueischemic heart diseaseatrial fibrillation |
| spellingShingle | Inga Andriulė Dalia Pangonytė Asfree Gwanyanya Dainius Karčiauskas Kanigula Mubagwa Regina Mačianskienė Detection of TRPM6 and TRPM7 Proteins in Normal and Diseased Cardiac Atrial Tissue and Isolated Cardiomyocytes International Journal of Molecular Sciences TRPM6 and TRPM7 channels atrial myocyte and tissue ischemic heart disease atrial fibrillation |
| title | Detection of TRPM6 and TRPM7 Proteins in Normal and Diseased Cardiac Atrial Tissue and Isolated Cardiomyocytes |
| title_full | Detection of TRPM6 and TRPM7 Proteins in Normal and Diseased Cardiac Atrial Tissue and Isolated Cardiomyocytes |
| title_fullStr | Detection of TRPM6 and TRPM7 Proteins in Normal and Diseased Cardiac Atrial Tissue and Isolated Cardiomyocytes |
| title_full_unstemmed | Detection of TRPM6 and TRPM7 Proteins in Normal and Diseased Cardiac Atrial Tissue and Isolated Cardiomyocytes |
| title_short | Detection of TRPM6 and TRPM7 Proteins in Normal and Diseased Cardiac Atrial Tissue and Isolated Cardiomyocytes |
| title_sort | detection of trpm6 and trpm7 proteins in normal and diseased cardiac atrial tissue and isolated cardiomyocytes |
| topic | TRPM6 and TRPM7 channels atrial myocyte and tissue ischemic heart disease atrial fibrillation |
| url | https://www.mdpi.com/1422-0067/23/23/14860 |
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