A Five-Year Analysis of Antibiotic Resistance Trends among Bacteria Identified in Positive Urine Samples in a Tertiary Care Hospital from Bucharest, Romania
The rise of multidrug-resistant bacteria (MDR) has resulted in limited treatment options and poorer outcomes for patients. The objective of this study was to analyze the overall antibiotic resistance trends and distribution for pathogens identified in urine samples at the National Institute of Infec...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-02-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/13/2/160 |
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| author | Alina Maria Borcan Georgiana Radu Mădălina Simoiu Elena Liliana Costea Alexandru Rafila |
| author_facet | Alina Maria Borcan Georgiana Radu Mădălina Simoiu Elena Liliana Costea Alexandru Rafila |
| author_sort | Alina Maria Borcan |
| collection | DOAJ |
| description | The rise of multidrug-resistant bacteria (MDR) has resulted in limited treatment options and poorer outcomes for patients. The objective of this study was to analyze the overall antibiotic resistance trends and distribution for pathogens identified in urine samples at the National Institute of Infectious Diseases “Prof. Dr. Matei Balș” from Bucharest, Romania, over a 5-year period. Antibiotic susceptibility testing was performed using automatic systems and the disk diffusion method. ESBL- and carbapenemases-producing strains were identified using immunochromatography tests, and ROSCO Diagnostica kits were used for definitive confirmation. All results were interpreted according to EUCAST clinical breakpoints. Gram-negative rods (GNR) had overall resistance rates higher than 50% for penicillin and 40% for 3rd- and 4th-generation cephalosporins. <i>Escherichia coli</i> resistance to fosfomycin (3%) and nitrofurantoin (2%) remains low, and 33.30% of <i>E. coli</i>, 48% of <i>Klebsiella</i> spp., and 37% of <i>Pseudomonas aeruginosa</i> isolates were multidrug-resistant (MDR). All <i>Acinetobacter baumannii</i> isolates were MDR by the last year of the study. For Gram-positive cocci (GPC), 37% of all <i>Enterococcus faecium</i> strains and 2% of <i>Enterococcus faecalis</i> were vancomycin-resistant (VRE). <i>E. coli</i>’s incidence in UTIs’ etiology is on a downward trend. The incidence of <i>Klebsiella</i> spp. and GPCs is rising. Antibiotic stewardship strategies should be implemented after carefully considering regional variations in etiology and resistance trends. |
| first_indexed | 2024-03-07T22:44:36Z |
| format | Article |
| id | doaj.art-70df6ac4c635475890c39981bc44f7cd |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-07T22:44:36Z |
| publishDate | 2024-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-70df6ac4c635475890c39981bc44f7cd2024-02-23T15:05:07ZengMDPI AGAntibiotics2079-63822024-02-0113216010.3390/antibiotics13020160A Five-Year Analysis of Antibiotic Resistance Trends among Bacteria Identified in Positive Urine Samples in a Tertiary Care Hospital from Bucharest, RomaniaAlina Maria Borcan0Georgiana Radu1Mădălina Simoiu2Elena Liliana Costea3Alexandru Rafila4The National Institute of Infectious Diseases “Prof. Dr. Matei Balș”, 021105 Bucharest, RomaniaFaculty of Medicine, The University of Medicine and Pharmacy “Carol Davila”, 050474 Bucharest, RomaniaThe National Institute of Infectious Diseases “Prof. Dr. Matei Balș”, 021105 Bucharest, RomaniaFaculty of Medicine, The University of Medicine and Pharmacy “Carol Davila”, 050474 Bucharest, RomaniaThe National Institute of Infectious Diseases “Prof. Dr. Matei Balș”, 021105 Bucharest, RomaniaThe rise of multidrug-resistant bacteria (MDR) has resulted in limited treatment options and poorer outcomes for patients. The objective of this study was to analyze the overall antibiotic resistance trends and distribution for pathogens identified in urine samples at the National Institute of Infectious Diseases “Prof. Dr. Matei Balș” from Bucharest, Romania, over a 5-year period. Antibiotic susceptibility testing was performed using automatic systems and the disk diffusion method. ESBL- and carbapenemases-producing strains were identified using immunochromatography tests, and ROSCO Diagnostica kits were used for definitive confirmation. All results were interpreted according to EUCAST clinical breakpoints. Gram-negative rods (GNR) had overall resistance rates higher than 50% for penicillin and 40% for 3rd- and 4th-generation cephalosporins. <i>Escherichia coli</i> resistance to fosfomycin (3%) and nitrofurantoin (2%) remains low, and 33.30% of <i>E. coli</i>, 48% of <i>Klebsiella</i> spp., and 37% of <i>Pseudomonas aeruginosa</i> isolates were multidrug-resistant (MDR). All <i>Acinetobacter baumannii</i> isolates were MDR by the last year of the study. For Gram-positive cocci (GPC), 37% of all <i>Enterococcus faecium</i> strains and 2% of <i>Enterococcus faecalis</i> were vancomycin-resistant (VRE). <i>E. coli</i>’s incidence in UTIs’ etiology is on a downward trend. The incidence of <i>Klebsiella</i> spp. and GPCs is rising. Antibiotic stewardship strategies should be implemented after carefully considering regional variations in etiology and resistance trends.https://www.mdpi.com/2079-6382/13/2/160uropathogensurinary tract infectionantibiotic resistance |
| spellingShingle | Alina Maria Borcan Georgiana Radu Mădălina Simoiu Elena Liliana Costea Alexandru Rafila A Five-Year Analysis of Antibiotic Resistance Trends among Bacteria Identified in Positive Urine Samples in a Tertiary Care Hospital from Bucharest, Romania Antibiotics uropathogens urinary tract infection antibiotic resistance |
| title | A Five-Year Analysis of Antibiotic Resistance Trends among Bacteria Identified in Positive Urine Samples in a Tertiary Care Hospital from Bucharest, Romania |
| title_full | A Five-Year Analysis of Antibiotic Resistance Trends among Bacteria Identified in Positive Urine Samples in a Tertiary Care Hospital from Bucharest, Romania |
| title_fullStr | A Five-Year Analysis of Antibiotic Resistance Trends among Bacteria Identified in Positive Urine Samples in a Tertiary Care Hospital from Bucharest, Romania |
| title_full_unstemmed | A Five-Year Analysis of Antibiotic Resistance Trends among Bacteria Identified in Positive Urine Samples in a Tertiary Care Hospital from Bucharest, Romania |
| title_short | A Five-Year Analysis of Antibiotic Resistance Trends among Bacteria Identified in Positive Urine Samples in a Tertiary Care Hospital from Bucharest, Romania |
| title_sort | five year analysis of antibiotic resistance trends among bacteria identified in positive urine samples in a tertiary care hospital from bucharest romania |
| topic | uropathogens urinary tract infection antibiotic resistance |
| url | https://www.mdpi.com/2079-6382/13/2/160 |
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