Low Temperature Scanning Electron Microscopy (LTSEM) Findings on the Ultrastructure of <i>Trebouxia lynnae (Trebouxiophyceae</i>, Lichenized Microalgae)
The lichenized green microalga <i>Trebouxia lynnae</i> Barreno has been recently described and is considered a model organism for studying lichen chlorobionts. Its cellular ultrastructure has already been studied in detail by light, electron, and confocal microscopy, and its nuclear, chl...
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MDPI AG
2023-01-01
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| author | César Daniel Bordenave Francisco García-Breijo Ayelén Gazquez Lucía Muggia Pedro Carrasco Eva Barreno |
| author_facet | César Daniel Bordenave Francisco García-Breijo Ayelén Gazquez Lucía Muggia Pedro Carrasco Eva Barreno |
| author_sort | César Daniel Bordenave |
| collection | DOAJ |
| description | The lichenized green microalga <i>Trebouxia lynnae</i> Barreno has been recently described and is considered a model organism for studying lichen chlorobionts. Its cellular ultrastructure has already been studied in detail by light, electron, and confocal microscopy, and its nuclear, chloroplast and mitochondrial genomes have been sequenced and annotated. Here, we investigated in detail the ultrastructure of in vitro grown cultures of <i>T. lynnae</i> observed by Low Temperature Scanning Electron Microscopy (LTSEM) applying a protocol with minimum intervention over the biological samples. This methodology allowed for the discovery of ultrastructural features previously unseen in <i>Trebouxiophyceae</i> microalgae. In addition, original Transmission Electron Microscopy (TEM) images of <i>T. lynnae</i> were reinterpreted based on the new information provided by LTSEM. The nucleolar vacuole, dictyosomes, and endoplasmic reticulum were investigated and reported for the first time in <i>T. lynnae</i> and most likely in other <i>Trebouxia</i> lineages. |
| first_indexed | 2024-03-11T08:56:59Z |
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| institution | Directory Open Access Journal |
| issn | 1424-2818 |
| language | English |
| last_indexed | 2024-03-11T08:56:59Z |
| publishDate | 2023-01-01 |
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| spelling | doaj.art-70df72f6fb9a438494c1a8eedb9c5be42023-11-16T20:04:03ZengMDPI AGDiversity1424-28182023-01-0115217010.3390/d15020170Low Temperature Scanning Electron Microscopy (LTSEM) Findings on the Ultrastructure of <i>Trebouxia lynnae (Trebouxiophyceae</i>, Lichenized Microalgae)César Daniel Bordenave0Francisco García-Breijo1Ayelén Gazquez2Lucía Muggia3Pedro Carrasco4Eva Barreno5Instituto Cavanilles de Biodiversidad y Biología Evolutiva (ICBiBE), Botánica, Universitat de València, C/Dr. Moliner, 50, 46100 Burjassot, SpainDepartamento de Ecosistemas Agroforestales ETSIAMN, Universitat Politècnica de València, Camino de Vera s/n, 46022 València, SpainInstituto Cavanilles de Biodiversidad y Biología Evolutiva (ICBiBE), Botánica, Universitat de València, C/Dr. Moliner, 50, 46100 Burjassot, SpainDepartment of Life Sciences, University of Trieste, via L. Giorgieri 10, 34127 Trieste, ItalyInstituto de Biotecnología y Biomedicina (BIOTECMED), Universitat de València, 46100 Burjassot, SpainInstituto Cavanilles de Biodiversidad y Biología Evolutiva (ICBiBE), Botánica, Universitat de València, C/Dr. Moliner, 50, 46100 Burjassot, SpainThe lichenized green microalga <i>Trebouxia lynnae</i> Barreno has been recently described and is considered a model organism for studying lichen chlorobionts. Its cellular ultrastructure has already been studied in detail by light, electron, and confocal microscopy, and its nuclear, chloroplast and mitochondrial genomes have been sequenced and annotated. Here, we investigated in detail the ultrastructure of in vitro grown cultures of <i>T. lynnae</i> observed by Low Temperature Scanning Electron Microscopy (LTSEM) applying a protocol with minimum intervention over the biological samples. This methodology allowed for the discovery of ultrastructural features previously unseen in <i>Trebouxiophyceae</i> microalgae. In addition, original Transmission Electron Microscopy (TEM) images of <i>T. lynnae</i> were reinterpreted based on the new information provided by LTSEM. The nucleolar vacuole, dictyosomes, and endoplasmic reticulum were investigated and reported for the first time in <i>T. lynnae</i> and most likely in other <i>Trebouxia</i> lineages.https://www.mdpi.com/1424-2818/15/2/170axenic cultureslow temperature scanning electron microscopyphycobionttransmission electron microscopy |
| spellingShingle | César Daniel Bordenave Francisco García-Breijo Ayelén Gazquez Lucía Muggia Pedro Carrasco Eva Barreno Low Temperature Scanning Electron Microscopy (LTSEM) Findings on the Ultrastructure of <i>Trebouxia lynnae (Trebouxiophyceae</i>, Lichenized Microalgae) Diversity axenic cultures low temperature scanning electron microscopy phycobiont transmission electron microscopy |
| title | Low Temperature Scanning Electron Microscopy (LTSEM) Findings on the Ultrastructure of <i>Trebouxia lynnae (Trebouxiophyceae</i>, Lichenized Microalgae) |
| title_full | Low Temperature Scanning Electron Microscopy (LTSEM) Findings on the Ultrastructure of <i>Trebouxia lynnae (Trebouxiophyceae</i>, Lichenized Microalgae) |
| title_fullStr | Low Temperature Scanning Electron Microscopy (LTSEM) Findings on the Ultrastructure of <i>Trebouxia lynnae (Trebouxiophyceae</i>, Lichenized Microalgae) |
| title_full_unstemmed | Low Temperature Scanning Electron Microscopy (LTSEM) Findings on the Ultrastructure of <i>Trebouxia lynnae (Trebouxiophyceae</i>, Lichenized Microalgae) |
| title_short | Low Temperature Scanning Electron Microscopy (LTSEM) Findings on the Ultrastructure of <i>Trebouxia lynnae (Trebouxiophyceae</i>, Lichenized Microalgae) |
| title_sort | low temperature scanning electron microscopy ltsem findings on the ultrastructure of i trebouxia lynnae trebouxiophyceae i lichenized microalgae |
| topic | axenic cultures low temperature scanning electron microscopy phycobiont transmission electron microscopy |
| url | https://www.mdpi.com/1424-2818/15/2/170 |
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