Detection and genetic characterization of novel infectious bronchitis viruses from recent outbreaks in broiler and layer chicken flocks in southern China, 2021
Avian infectious bronchitis virus (IBV) is a prevalent RNA virus that causes respiratory distress, nephritis, salpingitis, and egg production decline in chickens, resulting in significant economic loss. IBV is composed of complex genotypes and serotypes, which poses a great challenge for disease con...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-10-01
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| Series: | Poultry Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579122003728 |
| _version_ | 1811271843069296640 |
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| author | Sheng Yuan Qing Cheng Jinyue Guo Zhili Li Jing Yang Congying Wang Zhipeng Liang Xinyu Zhang Hai Yu Yong Li Shujian Huang Feng Wen |
| author_facet | Sheng Yuan Qing Cheng Jinyue Guo Zhili Li Jing Yang Congying Wang Zhipeng Liang Xinyu Zhang Hai Yu Yong Li Shujian Huang Feng Wen |
| author_sort | Sheng Yuan |
| collection | DOAJ |
| description | Avian infectious bronchitis virus (IBV) is a prevalent RNA virus that causes respiratory distress, nephritis, salpingitis, and egg production decline in chickens, resulting in significant economic loss. IBV is composed of complex genotypes and serotypes, which poses a great challenge for disease control. The current study reports 2 IBV outbreaks which were characterized by respiratory symptoms in IBV vaccinated commercial broilers and layers in Guangdong, China, in 2021. Two IBV strains, ZH01 and HH09, were identified via a RT-PCR assay through targeting the N gene and further characterization through full-length spike (S) gene sequence analysis. Phylogenetic analysis of S1 gene revealed that both ZH01 and HH09 belonged to the GI-19 lineage but contained a certain genetic distance from the GI-19 strain. Of note, the ZH01 and HH09 strains share a low homology of 70 and 86%, respectively, with common vaccine strains (H120), resulting in low vaccine protection. Further recombination analysis based on the S1 sequence suggested the newly identified IBV strains emerged through an intragroup recombination events between CK/CH/SCDY2003-2 and I0305/19 from G1-19 lineage. In addition, a number of novel mutations such as T273I, T292A, and S331K were found in the emerging IBV strains. Taken together, this study reports the genetic characteristics of 2 recent IBV outbreaks in southern China and emphasizes the urgent need for enhanced surveillance and development of novel vaccines for the control of IBV. |
| first_indexed | 2024-04-12T22:29:32Z |
| format | Article |
| id | doaj.art-70e48c1a6d7a46338d9d9c8e067385a8 |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-04-12T22:29:32Z |
| publishDate | 2022-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj.art-70e48c1a6d7a46338d9d9c8e067385a82022-12-22T03:14:02ZengElsevierPoultry Science0032-57912022-10-0110110102082Detection and genetic characterization of novel infectious bronchitis viruses from recent outbreaks in broiler and layer chicken flocks in southern China, 2021Sheng Yuan0Qing Cheng1Jinyue Guo2Zhili Li3Jing Yang4Congying Wang5Zhipeng Liang6Xinyu Zhang7Hai Yu8Yong Li9Shujian Huang10Feng Wen11College of Life Science and Engineering, Foshan University, Foshan, 528231, Guangdong, ChinaCollege of Life Science and Engineering, Foshan University, Foshan, 528231, Guangdong, ChinaCollege of Life Science and Engineering, Foshan University, Foshan, 528231, Guangdong, ChinaCollege of Life Science and Engineering, Foshan University, Foshan, 528231, Guangdong, ChinaCollege of Life Science and Engineering, Foshan University, Foshan, 528231, Guangdong, ChinaCollege of Life Science and Engineering, Foshan University, Foshan, 528231, Guangdong, ChinaCollege of Life Science and Engineering, Foshan University, Foshan, 528231, Guangdong, ChinaCollege of Life Science and Engineering, Foshan University, Foshan, 528231, Guangdong, ChinaShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, ChinaCollege of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, Jiangxi, ChinaCollege of Life Science and Engineering, Foshan University, Foshan, 528231, Guangdong, ChinaCollege of Life Science and Engineering, Foshan University, Foshan, 528231, Guangdong, China; Corresponding author:Avian infectious bronchitis virus (IBV) is a prevalent RNA virus that causes respiratory distress, nephritis, salpingitis, and egg production decline in chickens, resulting in significant economic loss. IBV is composed of complex genotypes and serotypes, which poses a great challenge for disease control. The current study reports 2 IBV outbreaks which were characterized by respiratory symptoms in IBV vaccinated commercial broilers and layers in Guangdong, China, in 2021. Two IBV strains, ZH01 and HH09, were identified via a RT-PCR assay through targeting the N gene and further characterization through full-length spike (S) gene sequence analysis. Phylogenetic analysis of S1 gene revealed that both ZH01 and HH09 belonged to the GI-19 lineage but contained a certain genetic distance from the GI-19 strain. Of note, the ZH01 and HH09 strains share a low homology of 70 and 86%, respectively, with common vaccine strains (H120), resulting in low vaccine protection. Further recombination analysis based on the S1 sequence suggested the newly identified IBV strains emerged through an intragroup recombination events between CK/CH/SCDY2003-2 and I0305/19 from G1-19 lineage. In addition, a number of novel mutations such as T273I, T292A, and S331K were found in the emerging IBV strains. Taken together, this study reports the genetic characteristics of 2 recent IBV outbreaks in southern China and emphasizes the urgent need for enhanced surveillance and development of novel vaccines for the control of IBV.http://www.sciencedirect.com/science/article/pii/S0032579122003728infectious bronchitis virusS1 geneavian coronavirusphylogenetic analysisG1-19 genotype |
| spellingShingle | Sheng Yuan Qing Cheng Jinyue Guo Zhili Li Jing Yang Congying Wang Zhipeng Liang Xinyu Zhang Hai Yu Yong Li Shujian Huang Feng Wen Detection and genetic characterization of novel infectious bronchitis viruses from recent outbreaks in broiler and layer chicken flocks in southern China, 2021 Poultry Science infectious bronchitis virus S1 gene avian coronavirus phylogenetic analysis G1-19 genotype |
| title | Detection and genetic characterization of novel infectious bronchitis viruses from recent outbreaks in broiler and layer chicken flocks in southern China, 2021 |
| title_full | Detection and genetic characterization of novel infectious bronchitis viruses from recent outbreaks in broiler and layer chicken flocks in southern China, 2021 |
| title_fullStr | Detection and genetic characterization of novel infectious bronchitis viruses from recent outbreaks in broiler and layer chicken flocks in southern China, 2021 |
| title_full_unstemmed | Detection and genetic characterization of novel infectious bronchitis viruses from recent outbreaks in broiler and layer chicken flocks in southern China, 2021 |
| title_short | Detection and genetic characterization of novel infectious bronchitis viruses from recent outbreaks in broiler and layer chicken flocks in southern China, 2021 |
| title_sort | detection and genetic characterization of novel infectious bronchitis viruses from recent outbreaks in broiler and layer chicken flocks in southern china 2021 |
| topic | infectious bronchitis virus S1 gene avian coronavirus phylogenetic analysis G1-19 genotype |
| url | http://www.sciencedirect.com/science/article/pii/S0032579122003728 |
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