Rapid Matching Antibodies Pair and Fast Detecting Melioidosis with Fluorescent Immunochromatographic Test Strips
The high infectivity, difficulty to diagnose, and high drug resistance of melioidosis limited the timeliness of treatment. Lateral flow assay (LFA) was operated in this research to provide an instant diagnosis method, and a fast antibody rapid matching test strategy based on LFA was developed to sel...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-06-01
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| Series: | Chemosensors |
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| Online Access: | https://www.mdpi.com/2227-9040/11/6/351 |
| _version_ | 1797595533043302400 |
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| author | Yi-Zhi Lin Ting-Ting Zhou Jin Zhu Shou-Nian Ding |
| author_facet | Yi-Zhi Lin Ting-Ting Zhou Jin Zhu Shou-Nian Ding |
| author_sort | Yi-Zhi Lin |
| collection | DOAJ |
| description | The high infectivity, difficulty to diagnose, and high drug resistance of melioidosis limited the timeliness of treatment. Lateral flow assay (LFA) was operated in this research to provide an instant diagnosis method, and a fast antibody rapid matching test strategy based on LFA was developed to select the most sensitive and specific pair of antibodies. Compared to the traditional ELISA method, the new matching strategy limits the pairing time to 3 h without any complex instruments. The rapid pairing test strategy is a universal strategy that is suitable for various sandwich immune antigen pairings. To fasten the test of the test strips, dry fluorescence immunoassay analyzer (DFIA) was designed and applied. The equipment also simplifies the process of data acquisition. Finally, the concentration gradient test was operated, and the detection lines and limits were presented. |
| first_indexed | 2024-03-11T02:38:51Z |
| format | Article |
| id | doaj.art-70e61e5d4ccc4a4c9760df5e22b9bc22 |
| institution | Directory Open Access Journal |
| issn | 2227-9040 |
| language | English |
| last_indexed | 2024-03-11T02:38:51Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Chemosensors |
| spelling | doaj.art-70e61e5d4ccc4a4c9760df5e22b9bc222023-11-18T09:46:25ZengMDPI AGChemosensors2227-90402023-06-0111635110.3390/chemosensors11060351Rapid Matching Antibodies Pair and Fast Detecting Melioidosis with Fluorescent Immunochromatographic Test StripsYi-Zhi Lin0Ting-Ting Zhou1Jin Zhu2Shou-Nian Ding3Jiangsu Province Hi-Tech Key Laboratory for Bio-Medical Research, School of Chemistry and Chemical Engineering, Southeast University, Nanjing 211189, ChinaHuadong Medical Institute of Biotechniques, Nanjing 210002, ChinaHuadong Medical Institute of Biotechniques, Nanjing 210002, ChinaJiangsu Province Hi-Tech Key Laboratory for Bio-Medical Research, School of Chemistry and Chemical Engineering, Southeast University, Nanjing 211189, ChinaThe high infectivity, difficulty to diagnose, and high drug resistance of melioidosis limited the timeliness of treatment. Lateral flow assay (LFA) was operated in this research to provide an instant diagnosis method, and a fast antibody rapid matching test strategy based on LFA was developed to select the most sensitive and specific pair of antibodies. Compared to the traditional ELISA method, the new matching strategy limits the pairing time to 3 h without any complex instruments. The rapid pairing test strategy is a universal strategy that is suitable for various sandwich immune antigen pairings. To fasten the test of the test strips, dry fluorescence immunoassay analyzer (DFIA) was designed and applied. The equipment also simplifies the process of data acquisition. Finally, the concentration gradient test was operated, and the detection lines and limits were presented.https://www.mdpi.com/2227-9040/11/6/351melioidosisantibody matchingcarbon dotsfluorescence |
| spellingShingle | Yi-Zhi Lin Ting-Ting Zhou Jin Zhu Shou-Nian Ding Rapid Matching Antibodies Pair and Fast Detecting Melioidosis with Fluorescent Immunochromatographic Test Strips Chemosensors melioidosis antibody matching carbon dots fluorescence |
| title | Rapid Matching Antibodies Pair and Fast Detecting Melioidosis with Fluorescent Immunochromatographic Test Strips |
| title_full | Rapid Matching Antibodies Pair and Fast Detecting Melioidosis with Fluorescent Immunochromatographic Test Strips |
| title_fullStr | Rapid Matching Antibodies Pair and Fast Detecting Melioidosis with Fluorescent Immunochromatographic Test Strips |
| title_full_unstemmed | Rapid Matching Antibodies Pair and Fast Detecting Melioidosis with Fluorescent Immunochromatographic Test Strips |
| title_short | Rapid Matching Antibodies Pair and Fast Detecting Melioidosis with Fluorescent Immunochromatographic Test Strips |
| title_sort | rapid matching antibodies pair and fast detecting melioidosis with fluorescent immunochromatographic test strips |
| topic | melioidosis antibody matching carbon dots fluorescence |
| url | https://www.mdpi.com/2227-9040/11/6/351 |
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