Occurrence of extended-spectrum beta-lactamases in Enterobacteriaceae isolated from hospitalized patients in Curitiba, southern Brazil
Production of extended-spectrum beta-lactamases (ESBL) by enterobacteria is an important resistance mechanism against antimicrobial beta-lactamics. We tested 498 bacterial strains isolated from two tertiary-care teaching hospitals for ESBL production, using screening breakpoints for aztreonam and th...
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| Format: | Article |
| Language: | English |
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Elsevier
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| Series: | Brazilian Journal of Infectious Diseases |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702006000600006&lng=en&tlng=en |
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| author | Keite da Silva Nogueira Ilma Hiroko Higuti Agnaldo José do Nascimento Larissa Bail Terasawa Simone de Oliveira Adriana Pereira Matos Helena Aguilar Peres Homem de Mello de Souza Laura Lúcia Cogo Libera Maria Dalla Costa |
| author_facet | Keite da Silva Nogueira Ilma Hiroko Higuti Agnaldo José do Nascimento Larissa Bail Terasawa Simone de Oliveira Adriana Pereira Matos Helena Aguilar Peres Homem de Mello de Souza Laura Lúcia Cogo Libera Maria Dalla Costa |
| author_sort | Keite da Silva Nogueira |
| collection | DOAJ |
| description | Production of extended-spectrum beta-lactamases (ESBL) by enterobacteria is an important resistance mechanism against antimicrobial beta-lactamics. We tested 498 bacterial strains isolated from two tertiary-care teaching hospitals for ESBL production, using screening breakpoints for aztreonam and third generation cephalosporins, according to CLSI recommendations. Among these isolates, 155 were positive for the ESBL screening test, and 121 (78%) were confirmed by the clavulanic acid combination disk method. We found a high frequency of ESBL (24%) among Enterobacteriaceae, with a frequency of 57.4% for Klebsiella pneumoniae, 21.4% for Klebsiella oxytoca, and 7.2% for E. coli. In other members of Enterobacteriaceae, non-Klebsiella and non-E. coli, the prevalence was 21.6%. Ceftriaxone and cefotaxime showed a higher sensitivity in the screening test (99.2%) when compared to ceftazidime, aztreonam and cefpodoxime. However, cefotaxime/cefotaxime plus clavulanic acid showed a higher sensitivity in the confirmatory test (96.7%). |
| first_indexed | 2024-12-23T21:18:54Z |
| format | Article |
| id | doaj.art-70e9e4e947cf40e3918c86803c1a4559 |
| institution | Directory Open Access Journal |
| issn | 1678-4391 |
| language | English |
| last_indexed | 2024-12-23T21:18:54Z |
| publisher | Elsevier |
| record_format | Article |
| series | Brazilian Journal of Infectious Diseases |
| spelling | doaj.art-70e9e4e947cf40e3918c86803c1a45592022-12-21T17:30:49ZengElsevierBrazilian Journal of Infectious Diseases1678-439110639039510.1590/S1413-86702006000600006S1413-86702006000600006Occurrence of extended-spectrum beta-lactamases in Enterobacteriaceae isolated from hospitalized patients in Curitiba, southern BrazilKeite da Silva Nogueira0Ilma Hiroko Higuti1Agnaldo José do Nascimento2Larissa Bail Terasawa3Simone de Oliveira4Adriana Pereira Matos5Helena Aguilar Peres Homem de Mello de Souza6Laura Lúcia Cogo7Libera Maria Dalla Costa8Universidade Federal do ParanáUniversidade Federal do ParanáUniversidade Federal do ParanáUniversidade Federal do ParanáUniversidade Federal do ParanáPontifícia Universidade Católica do ParanáUniversidade Federal do ParanáUniversidade Federal do ParanáUniversidade Federal do ParanáProduction of extended-spectrum beta-lactamases (ESBL) by enterobacteria is an important resistance mechanism against antimicrobial beta-lactamics. We tested 498 bacterial strains isolated from two tertiary-care teaching hospitals for ESBL production, using screening breakpoints for aztreonam and third generation cephalosporins, according to CLSI recommendations. Among these isolates, 155 were positive for the ESBL screening test, and 121 (78%) were confirmed by the clavulanic acid combination disk method. We found a high frequency of ESBL (24%) among Enterobacteriaceae, with a frequency of 57.4% for Klebsiella pneumoniae, 21.4% for Klebsiella oxytoca, and 7.2% for E. coli. In other members of Enterobacteriaceae, non-Klebsiella and non-E. coli, the prevalence was 21.6%. Ceftriaxone and cefotaxime showed a higher sensitivity in the screening test (99.2%) when compared to ceftazidime, aztreonam and cefpodoxime. However, cefotaxime/cefotaxime plus clavulanic acid showed a higher sensitivity in the confirmatory test (96.7%).http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702006000600006&lng=en&tlng=enESBLbeta-lactamasesEnterobacteriaceae |
| spellingShingle | Keite da Silva Nogueira Ilma Hiroko Higuti Agnaldo José do Nascimento Larissa Bail Terasawa Simone de Oliveira Adriana Pereira Matos Helena Aguilar Peres Homem de Mello de Souza Laura Lúcia Cogo Libera Maria Dalla Costa Occurrence of extended-spectrum beta-lactamases in Enterobacteriaceae isolated from hospitalized patients in Curitiba, southern Brazil Brazilian Journal of Infectious Diseases ESBL beta-lactamases Enterobacteriaceae |
| title | Occurrence of extended-spectrum beta-lactamases in Enterobacteriaceae isolated from hospitalized patients in Curitiba, southern Brazil |
| title_full | Occurrence of extended-spectrum beta-lactamases in Enterobacteriaceae isolated from hospitalized patients in Curitiba, southern Brazil |
| title_fullStr | Occurrence of extended-spectrum beta-lactamases in Enterobacteriaceae isolated from hospitalized patients in Curitiba, southern Brazil |
| title_full_unstemmed | Occurrence of extended-spectrum beta-lactamases in Enterobacteriaceae isolated from hospitalized patients in Curitiba, southern Brazil |
| title_short | Occurrence of extended-spectrum beta-lactamases in Enterobacteriaceae isolated from hospitalized patients in Curitiba, southern Brazil |
| title_sort | occurrence of extended spectrum beta lactamases in enterobacteriaceae isolated from hospitalized patients in curitiba southern brazil |
| topic | ESBL beta-lactamases Enterobacteriaceae |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702006000600006&lng=en&tlng=en |
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