Establishment and application of a rapid diagnostic method for BVDV and IBRV using recombinase polymerase amplification-lateral flow device

Bovine Viral Diarrhea Virus (BVDV) and Infectious Bovine Rhinotracheitis Virus (IBRV) are the two most prevalent infectious diseases in cattle. They both can cause persistent infection and immunosuppression, resulting in significant economic losses in the livestock industry. Therefore, rapid detecti...

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Main Authors: Yan Wang, Jinyuan Shang, Zhijie Li, Ao Zhang, Yuening Cheng
Format: Article
Language:English
Published: Frontiers Media S.A. 2024-03-01
Series:Frontiers in Veterinary Science
Subjects:
Online Access:https://www.frontiersin.org/articles/10.3389/fvets.2024.1360504/full
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author Yan Wang
Jinyuan Shang
Zhijie Li
Ao Zhang
Yuening Cheng
author_facet Yan Wang
Jinyuan Shang
Zhijie Li
Ao Zhang
Yuening Cheng
author_sort Yan Wang
collection DOAJ
description Bovine Viral Diarrhea Virus (BVDV) and Infectious Bovine Rhinotracheitis Virus (IBRV) are the two most prevalent infectious diseases in cattle. They both can cause persistent infection and immunosuppression, resulting in significant economic losses in the livestock industry. Therefore, rapid detection of early BVDV and IBRV infections is crucial. In this study, a method for the rapid detection of BVDV and IBRV was established by using recombinase polymerase amplification (RPA) combined with lateral flow device (LFD). By optimizing the temperature and time conditions of the RPA reaction, the sensitivity, specificity, and clinical performance were evaluated. The results indicated that the RPA reaction could be completed at 40°C within 25 min. The LOD for BVDV and IBRV by RPA-LFD were 5.1 × 101 copies/μL and 6.65 × 101 copies/μL, respectively, with no cross-reactivity observed with other viruses such as CSFV, BRSV, BPIV3, BRV, and BCoV. Testing of 32 clinical samples showed consistent results between RPA-LFD and qPCR. The RPA-LFD method established in this study can be used for the rapid clinical detection of BVDV and IBRV, which providing a rapid and convenient molecular biology approach for on-site rapid detection and epidemiological investigations. Simultaneously, it offers technical support for the prevention and control of these viruses.
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spelling doaj.art-70ebefa0c7ae478e9484e5136e3e58cc2024-03-27T11:05:07ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692024-03-011110.3389/fvets.2024.13605041360504Establishment and application of a rapid diagnostic method for BVDV and IBRV using recombinase polymerase amplification-lateral flow deviceYan WangJinyuan ShangZhijie LiAo ZhangYuening ChengBovine Viral Diarrhea Virus (BVDV) and Infectious Bovine Rhinotracheitis Virus (IBRV) are the two most prevalent infectious diseases in cattle. They both can cause persistent infection and immunosuppression, resulting in significant economic losses in the livestock industry. Therefore, rapid detection of early BVDV and IBRV infections is crucial. In this study, a method for the rapid detection of BVDV and IBRV was established by using recombinase polymerase amplification (RPA) combined with lateral flow device (LFD). By optimizing the temperature and time conditions of the RPA reaction, the sensitivity, specificity, and clinical performance were evaluated. The results indicated that the RPA reaction could be completed at 40°C within 25 min. The LOD for BVDV and IBRV by RPA-LFD were 5.1 × 101 copies/μL and 6.65 × 101 copies/μL, respectively, with no cross-reactivity observed with other viruses such as CSFV, BRSV, BPIV3, BRV, and BCoV. Testing of 32 clinical samples showed consistent results between RPA-LFD and qPCR. The RPA-LFD method established in this study can be used for the rapid clinical detection of BVDV and IBRV, which providing a rapid and convenient molecular biology approach for on-site rapid detection and epidemiological investigations. Simultaneously, it offers technical support for the prevention and control of these viruses.https://www.frontiersin.org/articles/10.3389/fvets.2024.1360504/fullBovine Viral Diarrhea VirusInfectious Bovine Rhinotracheitis Virusreverse transcriptase recombinase aided amplificationlateral flow dipstickrapid detection
spellingShingle Yan Wang
Jinyuan Shang
Zhijie Li
Ao Zhang
Yuening Cheng
Establishment and application of a rapid diagnostic method for BVDV and IBRV using recombinase polymerase amplification-lateral flow device
Frontiers in Veterinary Science
Bovine Viral Diarrhea Virus
Infectious Bovine Rhinotracheitis Virus
reverse transcriptase recombinase aided amplification
lateral flow dipstick
rapid detection
title Establishment and application of a rapid diagnostic method for BVDV and IBRV using recombinase polymerase amplification-lateral flow device
title_full Establishment and application of a rapid diagnostic method for BVDV and IBRV using recombinase polymerase amplification-lateral flow device
title_fullStr Establishment and application of a rapid diagnostic method for BVDV and IBRV using recombinase polymerase amplification-lateral flow device
title_full_unstemmed Establishment and application of a rapid diagnostic method for BVDV and IBRV using recombinase polymerase amplification-lateral flow device
title_short Establishment and application of a rapid diagnostic method for BVDV and IBRV using recombinase polymerase amplification-lateral flow device
title_sort establishment and application of a rapid diagnostic method for bvdv and ibrv using recombinase polymerase amplification lateral flow device
topic Bovine Viral Diarrhea Virus
Infectious Bovine Rhinotracheitis Virus
reverse transcriptase recombinase aided amplification
lateral flow dipstick
rapid detection
url https://www.frontiersin.org/articles/10.3389/fvets.2024.1360504/full
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