The dynamic changes of HBV quasispecies diversity in infancy after immunoprophylaxis failure: a prospective cohort study
Abstract Background Previous works have observed that younger infants with chronic hepatitis B virus (HBV) infection are more responsive to antiviral treatment. However, the underlying mechanism remains unclear. In this study, the dynamic changes of HBV quasispecies in infants with immunoprophylaxis...
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BMC
2021-11-01
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| Series: | Virology Journal |
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| Online Access: | https://doi.org/10.1186/s12985-021-01707-9 |
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| author | Yi Li Yiwei Xiao Lili Li Yarong Song Xiangjun Zhai Jianxun Liu Zhongping Duan Ling Yan Feng Ding Jia Liu Liguo Zhu Jie Jiang Huaibin Zou Lingxiang Li Caihong Liang Jie Wang Jie Li |
| author_facet | Yi Li Yiwei Xiao Lili Li Yarong Song Xiangjun Zhai Jianxun Liu Zhongping Duan Ling Yan Feng Ding Jia Liu Liguo Zhu Jie Jiang Huaibin Zou Lingxiang Li Caihong Liang Jie Wang Jie Li |
| author_sort | Yi Li |
| collection | DOAJ |
| description | Abstract Background Previous works have observed that younger infants with chronic hepatitis B virus (HBV) infection are more responsive to antiviral treatment. However, the underlying mechanism remains unclear. In this study, the dynamic changes of HBV quasispecies in infants with immunoprophylaxis failure were investigated to provide virological explanations for clinical management on infantile antiviral therapy. Methods Thirteen 7-month-old infants with immunoprophylaxis failure and their mothers were enrolled from a prospective cohort, and 8 of them were followed up to 3 years old. The sequences of HBV quasispecies were analyzed by the full-length genome clone-based sequencing, and compared among mothers and their infants at different ages. Results The results revealed that the complexity, mutation frequency and genetic distance of HBV quasispecies decreased significantly at full-length, partial open reading frames and regulatory regions of HBV genome at nucleotide level in 7-month-old infants comparing with their mothers, whereas increased significantly to near the maternal level when infants grew up to 3 years old. Furthermore, similar changes were also found in Core, PreS2, RT and P regions of HBV genome at amino acid level, especially for potential NAs-resistant mutants in RT region and immune-escape mutants in Core and PreS2 regions. Conclusions This study uncovered the evolution of HBV quasispecies in infancy after mother-to-child transmission, which may provide the virological evidence for explaning that younger children are more responsive to antiviral therapy. |
| first_indexed | 2024-12-15T00:18:24Z |
| format | Article |
| id | doaj.art-70ec5185eb9d477aa20ec95d85694a28 |
| institution | Directory Open Access Journal |
| issn | 1743-422X |
| language | English |
| last_indexed | 2024-12-15T00:18:24Z |
| publishDate | 2021-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Virology Journal |
| spelling | doaj.art-70ec5185eb9d477aa20ec95d85694a282022-12-21T22:42:23ZengBMCVirology Journal1743-422X2021-11-0118111110.1186/s12985-021-01707-9The dynamic changes of HBV quasispecies diversity in infancy after immunoprophylaxis failure: a prospective cohort studyYi Li0Yiwei Xiao1Lili Li2Yarong Song3Xiangjun Zhai4Jianxun Liu5Zhongping Duan6Ling Yan7Feng Ding8Jia Liu9Liguo Zhu10Jie Jiang11Huaibin Zou12Lingxiang Li13Caihong Liang14Jie Wang15Jie Li16Department of Microbiology and Infectious Disease Center, School of Basic Medical Sciences, Peking University Health Science CenterDepartment of Microbiology and Infectious Disease Center, School of Basic Medical Sciences, Peking University Health Science CenterDepartment of Microbiology and Infectious Disease Center, School of Basic Medical Sciences, Peking University Health Science CenterDepartment of Microbiology and Infectious Disease Center, School of Basic Medical Sciences, Peking University Health Science CenterJiangsu Provincial Center for Disease Control and PreventionZhengzhou Municipal Center for Disease Control and PreventionBeijing Youan Hospital, Capital Medical UniversityDepartment of Microbiology and Infectious Disease Center, School of Basic Medical Sciences, Peking University Health Science CenterDepartment of Microbiology and Infectious Disease Center, School of Basic Medical Sciences, Peking University Health Science CenterDepartment of Microbiology and Infectious Disease Center, School of Basic Medical Sciences, Peking University Health Science CenterJiangsu Provincial Center for Disease Control and PreventionJiangsu Provincial Center for Disease Control and PreventionBeijing Youan Hospital, Capital Medical UniversityGongyi City Maternal and Child Health HospitalZhongmu County Maternal and Child Health HospitalDepartment of Microbiology and Infectious Disease Center, School of Basic Medical Sciences, Peking University Health Science CenterDepartment of Microbiology and Infectious Disease Center, School of Basic Medical Sciences, Peking University Health Science CenterAbstract Background Previous works have observed that younger infants with chronic hepatitis B virus (HBV) infection are more responsive to antiviral treatment. However, the underlying mechanism remains unclear. In this study, the dynamic changes of HBV quasispecies in infants with immunoprophylaxis failure were investigated to provide virological explanations for clinical management on infantile antiviral therapy. Methods Thirteen 7-month-old infants with immunoprophylaxis failure and their mothers were enrolled from a prospective cohort, and 8 of them were followed up to 3 years old. The sequences of HBV quasispecies were analyzed by the full-length genome clone-based sequencing, and compared among mothers and their infants at different ages. Results The results revealed that the complexity, mutation frequency and genetic distance of HBV quasispecies decreased significantly at full-length, partial open reading frames and regulatory regions of HBV genome at nucleotide level in 7-month-old infants comparing with their mothers, whereas increased significantly to near the maternal level when infants grew up to 3 years old. Furthermore, similar changes were also found in Core, PreS2, RT and P regions of HBV genome at amino acid level, especially for potential NAs-resistant mutants in RT region and immune-escape mutants in Core and PreS2 regions. Conclusions This study uncovered the evolution of HBV quasispecies in infancy after mother-to-child transmission, which may provide the virological evidence for explaning that younger children are more responsive to antiviral therapy.https://doi.org/10.1186/s12985-021-01707-9Hepatitis B virusQuasispeciesMother-to-child transmissionImmunoprophylaxis failureInfantile antiviral therapy |
| spellingShingle | Yi Li Yiwei Xiao Lili Li Yarong Song Xiangjun Zhai Jianxun Liu Zhongping Duan Ling Yan Feng Ding Jia Liu Liguo Zhu Jie Jiang Huaibin Zou Lingxiang Li Caihong Liang Jie Wang Jie Li The dynamic changes of HBV quasispecies diversity in infancy after immunoprophylaxis failure: a prospective cohort study Virology Journal Hepatitis B virus Quasispecies Mother-to-child transmission Immunoprophylaxis failure Infantile antiviral therapy |
| title | The dynamic changes of HBV quasispecies diversity in infancy after immunoprophylaxis failure: a prospective cohort study |
| title_full | The dynamic changes of HBV quasispecies diversity in infancy after immunoprophylaxis failure: a prospective cohort study |
| title_fullStr | The dynamic changes of HBV quasispecies diversity in infancy after immunoprophylaxis failure: a prospective cohort study |
| title_full_unstemmed | The dynamic changes of HBV quasispecies diversity in infancy after immunoprophylaxis failure: a prospective cohort study |
| title_short | The dynamic changes of HBV quasispecies diversity in infancy after immunoprophylaxis failure: a prospective cohort study |
| title_sort | dynamic changes of hbv quasispecies diversity in infancy after immunoprophylaxis failure a prospective cohort study |
| topic | Hepatitis B virus Quasispecies Mother-to-child transmission Immunoprophylaxis failure Infantile antiviral therapy |
| url | https://doi.org/10.1186/s12985-021-01707-9 |
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