The Effects of Dietary Supplements of Calcium, Vitamin D and Estrogen Hormone on Serum Levels of OPG and RANKL Cytokines and their Relationship with Increased Bone Density in Rats
Introduction: Osteoprotegerin (OPG)-Receptor activator of nuclear factor kappa-B ligand (RANKL) pathway is one of the contributing factors in the regulation of osteogenesis and bone resorption routes. Aim: The purpose of this study was to evaluate the effects of various dietary supplements on t...
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| Format: | Article |
| Language: | English |
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JCDR Research and Publications Private Limited
2016-09-01
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| Series: | Journal of Clinical and Diagnostic Research |
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| Online Access: | https://jcdr.net/articles/PDF/8433/18648_CE(RA1)_F(T)_PF1(ASAK)_PFA(AK)_PF2(PAG).pdf |
| _version_ | 1818316399799435264 |
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| author | Fatemeh Piri Afra Khosravi Ardeshir Moayeri Ayat Moradipour Siamak Derakhshan |
| author_facet | Fatemeh Piri Afra Khosravi Ardeshir Moayeri Ayat Moradipour Siamak Derakhshan |
| author_sort | Fatemeh Piri |
| collection | DOAJ |
| description | Introduction: Osteoprotegerin (OPG)-Receptor activator of
nuclear factor kappa-B ligand (RANKL) pathway is one of the
contributing factors in the regulation of osteogenesis and bone
resorption routes.
Aim: The purpose of this study was to evaluate the effects of
various dietary supplements on this pathway.
Materials and Methods: The samples for this study (24 newborn
rats) were divided in three groups according to the experiment
applied for each group. Rats were given special diet according
to their group plan for six weeks. Blood samples were collected
to measure their serum levels of OPG and RANKL and all organs
of rats were used to measure their bone density too. The results
were analysed using appropriate statistical analysing tests.
Results: Levels of whole-body bone mineral density in calcium
plus vitamin D plus Estrogen (Ca + D + E) group and calcium plus
vitamin D (Ca + D) group were significantly increased compared
to control group. Mineral density was highest in calcium plus
vitamin D plus Estrogen group and was about 0.1357 g/cm².
RANKL had a significant decrease in calcium plus vitamin D plus
Estrogen group compared to control and calcium plus vitamin
D groups. There was a significant increase in the mean calcium
and OPG in both experimental groups rather than control. Also,
significant increase in estrogen was observed in Ca + D group
than the control group.
Conclusion: The results showed that intake of calcium and
vitamin D and estrogen at determined dose led to an increase in
OPG and RANKL cytokines reduction which ultimately led to an
increase in bone mineral density. But Ca, D and E synergies were
more effective in increasing bone mineral density compared to
only the use of Ca and D. |
| first_indexed | 2024-12-13T09:20:49Z |
| format | Article |
| id | doaj.art-70ed1a087e4e4c3db2e75e1daa31abc8 |
| institution | Directory Open Access Journal |
| issn | 2249-782X 0973-709X |
| language | English |
| last_indexed | 2024-12-13T09:20:49Z |
| publishDate | 2016-09-01 |
| publisher | JCDR Research and Publications Private Limited |
| record_format | Article |
| series | Journal of Clinical and Diagnostic Research |
| spelling | doaj.art-70ed1a087e4e4c3db2e75e1daa31abc82022-12-21T23:52:44ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2016-09-01109AF01AF0410.7860/JCDR/2016/18648.8433The Effects of Dietary Supplements of Calcium, Vitamin D and Estrogen Hormone on Serum Levels of OPG and RANKL Cytokines and their Relationship with Increased Bone Density in RatsFatemeh Piri0Afra Khosravi1Ardeshir Moayeri2Ayat Moradipour3Siamak Derakhshan4Faculty of Medicine, Department of Anatomy, Ilam University of Medical Sciences. Ilam, Iran.Professor, Department of Immunology, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, Iran.Assistant Professor, Department of Anatomy, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, Iran.Student, Departmant of Microbiology, Borujerd Branch, Islamic Azad University, Borujerd, Iran.Assistant Professor, Department of Radiology and Nuclear Medicine, Faculty of Medicine, Kurdestan University of Medical Sciences, Kurdestan, Iran.Introduction: Osteoprotegerin (OPG)-Receptor activator of nuclear factor kappa-B ligand (RANKL) pathway is one of the contributing factors in the regulation of osteogenesis and bone resorption routes. Aim: The purpose of this study was to evaluate the effects of various dietary supplements on this pathway. Materials and Methods: The samples for this study (24 newborn rats) were divided in three groups according to the experiment applied for each group. Rats were given special diet according to their group plan for six weeks. Blood samples were collected to measure their serum levels of OPG and RANKL and all organs of rats were used to measure their bone density too. The results were analysed using appropriate statistical analysing tests. Results: Levels of whole-body bone mineral density in calcium plus vitamin D plus Estrogen (Ca + D + E) group and calcium plus vitamin D (Ca + D) group were significantly increased compared to control group. Mineral density was highest in calcium plus vitamin D plus Estrogen group and was about 0.1357 g/cm². RANKL had a significant decrease in calcium plus vitamin D plus Estrogen group compared to control and calcium plus vitamin D groups. There was a significant increase in the mean calcium and OPG in both experimental groups rather than control. Also, significant increase in estrogen was observed in Ca + D group than the control group. Conclusion: The results showed that intake of calcium and vitamin D and estrogen at determined dose led to an increase in OPG and RANKL cytokines reduction which ultimately led to an increase in bone mineral density. But Ca, D and E synergies were more effective in increasing bone mineral density compared to only the use of Ca and D.https://jcdr.net/articles/PDF/8433/18648_CE(RA1)_F(T)_PF1(ASAK)_PFA(AK)_PF2(PAG).pdfestradiolosteoblastosteoclast |
| spellingShingle | Fatemeh Piri Afra Khosravi Ardeshir Moayeri Ayat Moradipour Siamak Derakhshan The Effects of Dietary Supplements of Calcium, Vitamin D and Estrogen Hormone on Serum Levels of OPG and RANKL Cytokines and their Relationship with Increased Bone Density in Rats Journal of Clinical and Diagnostic Research estradiol osteoblast osteoclast |
| title | The Effects of Dietary Supplements of Calcium, Vitamin D and Estrogen Hormone on Serum Levels of OPG and RANKL Cytokines and their Relationship with Increased Bone Density in Rats |
| title_full | The Effects of Dietary Supplements of Calcium, Vitamin D and Estrogen Hormone on Serum Levels of OPG and RANKL Cytokines and their Relationship with Increased Bone Density in Rats |
| title_fullStr | The Effects of Dietary Supplements of Calcium, Vitamin D and Estrogen Hormone on Serum Levels of OPG and RANKL Cytokines and their Relationship with Increased Bone Density in Rats |
| title_full_unstemmed | The Effects of Dietary Supplements of Calcium, Vitamin D and Estrogen Hormone on Serum Levels of OPG and RANKL Cytokines and their Relationship with Increased Bone Density in Rats |
| title_short | The Effects of Dietary Supplements of Calcium, Vitamin D and Estrogen Hormone on Serum Levels of OPG and RANKL Cytokines and their Relationship with Increased Bone Density in Rats |
| title_sort | effects of dietary supplements of calcium vitamin d and estrogen hormone on serum levels of opg and rankl cytokines and their relationship with increased bone density in rats |
| topic | estradiol osteoblast osteoclast |
| url | https://jcdr.net/articles/PDF/8433/18648_CE(RA1)_F(T)_PF1(ASAK)_PFA(AK)_PF2(PAG).pdf |
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