Action of neuwiedase, a metalloproteinase isolated from Bothrops neuwiedi venom, on skeletal muscle: an ultrastructural and immunocytochemistry study
The damaging effects of neuwiedase, a non-hemorrhagic snake venom metalloproteinase from P-I class, on gastrocnemius muscle are studied herein. Following neuwiedase injection, ultrastructural alterations were detected early showing disarrangement of skeletal muscle fibers (characterized by discontin...
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| Format: | Article |
| Language: | English |
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SciELO
2010-01-01
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| Series: | Journal of Venomous Animals and Toxins including Tropical Diseases |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000300013 |
| _version_ | 1818277206569254912 |
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| author | C Baldo MJ Ferreira DS Lopes LFM Izidoro AO Gomes EAV Ferro A Hamaguchi MI Homsi-Brandeburgo VM Rodrigues |
| author_facet | C Baldo MJ Ferreira DS Lopes LFM Izidoro AO Gomes EAV Ferro A Hamaguchi MI Homsi-Brandeburgo VM Rodrigues |
| author_sort | C Baldo |
| collection | DOAJ |
| description | The damaging effects of neuwiedase, a non-hemorrhagic snake venom metalloproteinase from P-I class, on gastrocnemius muscle are studied herein. Following neuwiedase injection, ultrastructural alterations were detected early showing disarrangement of skeletal muscle fibers (characterized by discontinuity of Z lines), mitochondrial swelling, and disruption of plasma membrane and basal lamina. Degradation of skeletal muscle and the appearance of an amorphous substance, primarily composed of cellular debris, were noted after 24 hours. The presence of neuwiedase at the injection site (detected by immunocytochemistry) revealed highly specific labeling of myofibril components of damaged myocytes. In addition, proteolysis of muscle proteins assayed through myofibrils extracted from gastrocnemius muscle indicated that neuwiedase provoked degradation of myofibrils, especially myosin. These results suggest that skeletal muscle damage, induced by neuwiedase, is probably due to its proteolytic action on myofibrils, which are responsible for the maintenance of the cellular architecture. |
| first_indexed | 2024-12-12T22:57:51Z |
| format | Article |
| id | doaj.art-70f08337aac74713a044f26b3e38e393 |
| institution | Directory Open Access Journal |
| issn | 1678-9199 |
| language | English |
| last_indexed | 2024-12-12T22:57:51Z |
| publishDate | 2010-01-01 |
| publisher | SciELO |
| record_format | Article |
| series | Journal of Venomous Animals and Toxins including Tropical Diseases |
| spelling | doaj.art-70f08337aac74713a044f26b3e38e3932022-12-22T00:08:53ZengSciELOJournal of Venomous Animals and Toxins including Tropical Diseases1678-91992010-01-0116346246910.1590/S1678-91992010000300013Action of neuwiedase, a metalloproteinase isolated from Bothrops neuwiedi venom, on skeletal muscle: an ultrastructural and immunocytochemistry studyC BaldoMJ FerreiraDS LopesLFM IzidoroAO GomesEAV FerroA HamaguchiMI Homsi-BrandeburgoVM RodriguesThe damaging effects of neuwiedase, a non-hemorrhagic snake venom metalloproteinase from P-I class, on gastrocnemius muscle are studied herein. Following neuwiedase injection, ultrastructural alterations were detected early showing disarrangement of skeletal muscle fibers (characterized by discontinuity of Z lines), mitochondrial swelling, and disruption of plasma membrane and basal lamina. Degradation of skeletal muscle and the appearance of an amorphous substance, primarily composed of cellular debris, were noted after 24 hours. The presence of neuwiedase at the injection site (detected by immunocytochemistry) revealed highly specific labeling of myofibril components of damaged myocytes. In addition, proteolysis of muscle proteins assayed through myofibrils extracted from gastrocnemius muscle indicated that neuwiedase provoked degradation of myofibrils, especially myosin. These results suggest that skeletal muscle damage, induced by neuwiedase, is probably due to its proteolytic action on myofibrils, which are responsible for the maintenance of the cellular architecture.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000300013skeletal muscleneuwiedasesnake venom metalloproteinasesBothrops neuwiedi |
| spellingShingle | C Baldo MJ Ferreira DS Lopes LFM Izidoro AO Gomes EAV Ferro A Hamaguchi MI Homsi-Brandeburgo VM Rodrigues Action of neuwiedase, a metalloproteinase isolated from Bothrops neuwiedi venom, on skeletal muscle: an ultrastructural and immunocytochemistry study Journal of Venomous Animals and Toxins including Tropical Diseases skeletal muscle neuwiedase snake venom metalloproteinases Bothrops neuwiedi |
| title | Action of neuwiedase, a metalloproteinase isolated from Bothrops neuwiedi venom, on skeletal muscle: an ultrastructural and immunocytochemistry study |
| title_full | Action of neuwiedase, a metalloproteinase isolated from Bothrops neuwiedi venom, on skeletal muscle: an ultrastructural and immunocytochemistry study |
| title_fullStr | Action of neuwiedase, a metalloproteinase isolated from Bothrops neuwiedi venom, on skeletal muscle: an ultrastructural and immunocytochemistry study |
| title_full_unstemmed | Action of neuwiedase, a metalloproteinase isolated from Bothrops neuwiedi venom, on skeletal muscle: an ultrastructural and immunocytochemistry study |
| title_short | Action of neuwiedase, a metalloproteinase isolated from Bothrops neuwiedi venom, on skeletal muscle: an ultrastructural and immunocytochemistry study |
| title_sort | action of neuwiedase a metalloproteinase isolated from bothrops neuwiedi venom on skeletal muscle an ultrastructural and immunocytochemistry study |
| topic | skeletal muscle neuwiedase snake venom metalloproteinases Bothrops neuwiedi |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000300013 |
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