Melatonin mitigates cold-induced damage to pepper seedlings by promoting redox homeostasis and regulating antioxidant profiling
This study assessed the influence of exogenous ME in the mitigation of cold damage in pepper seedlings. Melatonin (ME) is a dynamic molecule that helps plants cope with stress in several ways. Cold stress (CS) is one of the most important environmental factors that restrict plant growth and yield. P...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2024-03-01
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| Series: | Horticultural Plant Journal |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2468014123000262 |
| _version_ | 1797271327854297088 |
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| author | Muhammad Ahsan Altaf Yuanyuan Hao Huangying Shu Weiheng Jin Chuhao Chen Lin Li Yu Zhang Muhammad Ali Mumtaz Huizhen Fu Shanhan Cheng Guopeng Zhu Zhiwei Wang |
| author_facet | Muhammad Ahsan Altaf Yuanyuan Hao Huangying Shu Weiheng Jin Chuhao Chen Lin Li Yu Zhang Muhammad Ali Mumtaz Huizhen Fu Shanhan Cheng Guopeng Zhu Zhiwei Wang |
| author_sort | Muhammad Ahsan Altaf |
| collection | DOAJ |
| description | This study assessed the influence of exogenous ME in the mitigation of cold damage in pepper seedlings. Melatonin (ME) is a dynamic molecule that helps plants cope with stress in several ways. Cold stress (CS) is one of the most important environmental factors that restrict plant growth and yield. Pepper (Capsicum annuum L.) is a valuable commercial crop, highly sensitive to CS. Thus, identifying an efficient strategy to mitigate cold damage is critical for long-term pepper production. For this purpose, the roots of pepper seedlings were pretreated with ME (5 μmol · L−1) and exposed to CS for 7 d. The results indicated that CS suppressed pepper growth, hampered photosynthetic capacity, and damaged root architecture in pepper plants. In contrast, the production of reactive oxygen species (ROS), malondialdehyde (MDA), electrolyte leakage (EL), proline, and soluble sugars were enhanced in plants under CS. ME (5 μmol · L−1) pretreatment reduced the negative effects of CS by recovering plant growth, root traits, gas exchange elements, and pigment molecules compared to CS control treatment. Furthermore, ME application efficiently reduced oxidative stress markers [hydrogen peroxide (H2O2), superoxide ion (O2·–), EL, and MDA] while increasing proline and soluble sugar content in pepper leaves. ME application combined with CS further increased antioxidant enzymes and related gene expression. Collectively, our results confirmed the mitigating potential of ME supplementation for CS by maintaining pepper seedling growth, improving the photosynthesis apparatus, regulating pigments, and osmolyte content. |
| first_indexed | 2024-03-07T14:01:08Z |
| format | Article |
| id | doaj.art-70f12a22d8ca4d11bd39e23832ca2877 |
| institution | Directory Open Access Journal |
| issn | 2468-0141 |
| language | English |
| last_indexed | 2024-03-07T14:01:08Z |
| publishDate | 2024-03-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Horticultural Plant Journal |
| spelling | doaj.art-70f12a22d8ca4d11bd39e23832ca28772024-03-07T05:28:45ZengKeAi Communications Co., Ltd.Horticultural Plant Journal2468-01412024-03-01102532544Melatonin mitigates cold-induced damage to pepper seedlings by promoting redox homeostasis and regulating antioxidant profilingMuhammad Ahsan Altaf0Yuanyuan Hao1Huangying Shu2Weiheng Jin3Chuhao Chen4Lin Li5Yu Zhang6Muhammad Ali Mumtaz7Huizhen Fu8Shanhan Cheng9Guopeng Zhu10Zhiwei Wang11Key Laboratory for Quality Regulation of Tropical Horticultural Crops of Hainan Province, Sanya Nanfan Research Institute, Hainan University, Sanya, Hainan 572025, China; Key Laboratory for Quality Regulation of Tropical Horticultural Crops of Hainan Province, School of Horticulture, Hainan University, Haikou, Hainan 570228, China; Hainan Yazhou Bay Seed Laboratory, Sanya, Hainan 572025, ChinaKey Laboratory for Quality Regulation of Tropical Horticultural Crops of Hainan Province, Sanya Nanfan Research Institute, Hainan University, Sanya, Hainan 572025, China; Key Laboratory for Quality Regulation of Tropical Horticultural Crops of Hainan Province, School of Horticulture, Hainan University, Haikou, Hainan 570228, China; Hainan Yazhou Bay Seed Laboratory, Sanya, Hainan 572025, ChinaKey Laboratory for Quality Regulation of Tropical Horticultural Crops of Hainan Province, Sanya Nanfan Research Institute, Hainan University, Sanya, Hainan 572025, China; Key Laboratory for Quality Regulation of Tropical Horticultural Crops of Hainan Province, School of Horticulture, Hainan University, Haikou, Hainan 570228, ChinaKey Laboratory for Quality Regulation of Tropical Horticultural Crops of Hainan Province, Sanya Nanfan Research Institute, Hainan University, Sanya, Hainan 572025, China; Key Laboratory for Quality Regulation of Tropical Horticultural Crops of Hainan Province, School of Horticulture, Hainan University, Haikou, Hainan 570228, ChinaKey Laboratory for Quality Regulation of Tropical Horticultural Crops of Hainan Province, Sanya Nanfan Research Institute, Hainan University, Sanya, Hainan 572025, China; Key Laboratory for Quality Regulation of Tropical Horticultural Crops of Hainan Province, School of Horticulture, Hainan University, Haikou, Hainan 570228, ChinaKey Laboratory for Quality Regulation of Tropical Horticultural Crops of Hainan Province, Sanya Nanfan Research Institute, Hainan University, Sanya, Hainan 572025, China; Key Laboratory for Quality Regulation of Tropical Horticultural Crops of Hainan Province, School of Horticulture, Hainan University, Haikou, Hainan 570228, ChinaKey Laboratory for Quality Regulation of Tropical Horticultural Crops of Hainan Province, Sanya Nanfan Research Institute, Hainan University, Sanya, Hainan 572025, China; Key Laboratory for Quality Regulation of Tropical Horticultural Crops of Hainan Province, School of Horticulture, Hainan University, Haikou, Hainan 570228, ChinaKey Laboratory for Quality Regulation of Tropical Horticultural Crops of Hainan Province, Sanya Nanfan Research Institute, Hainan University, Sanya, Hainan 572025, China; Key Laboratory for Quality Regulation of Tropical Horticultural Crops of Hainan Province, School of Horticulture, Hainan University, Haikou, Hainan 570228, ChinaKey Laboratory for Quality Regulation of Tropical Horticultural Crops of Hainan Province, Sanya Nanfan Research Institute, Hainan University, Sanya, Hainan 572025, China; Key Laboratory for Quality Regulation of Tropical Horticultural Crops of Hainan Province, School of Horticulture, Hainan University, Haikou, Hainan 570228, ChinaKey Laboratory for Quality Regulation of Tropical Horticultural Crops of Hainan Province, Sanya Nanfan Research Institute, Hainan University, Sanya, Hainan 572025, China; Key Laboratory for Quality Regulation of Tropical Horticultural Crops of Hainan Province, School of Horticulture, Hainan University, Haikou, Hainan 570228, ChinaKey Laboratory for Quality Regulation of Tropical Horticultural Crops of Hainan Province, Sanya Nanfan Research Institute, Hainan University, Sanya, Hainan 572025, China; Key Laboratory for Quality Regulation of Tropical Horticultural Crops of Hainan Province, School of Horticulture, Hainan University, Haikou, Hainan 570228, ChinaKey Laboratory for Quality Regulation of Tropical Horticultural Crops of Hainan Province, Sanya Nanfan Research Institute, Hainan University, Sanya, Hainan 572025, China; Key Laboratory for Quality Regulation of Tropical Horticultural Crops of Hainan Province, School of Horticulture, Hainan University, Haikou, Hainan 570228, China; Hainan Yazhou Bay Seed Laboratory, Sanya, Hainan 572025, China; Corresponding author.This study assessed the influence of exogenous ME in the mitigation of cold damage in pepper seedlings. Melatonin (ME) is a dynamic molecule that helps plants cope with stress in several ways. Cold stress (CS) is one of the most important environmental factors that restrict plant growth and yield. Pepper (Capsicum annuum L.) is a valuable commercial crop, highly sensitive to CS. Thus, identifying an efficient strategy to mitigate cold damage is critical for long-term pepper production. For this purpose, the roots of pepper seedlings were pretreated with ME (5 μmol · L−1) and exposed to CS for 7 d. The results indicated that CS suppressed pepper growth, hampered photosynthetic capacity, and damaged root architecture in pepper plants. In contrast, the production of reactive oxygen species (ROS), malondialdehyde (MDA), electrolyte leakage (EL), proline, and soluble sugars were enhanced in plants under CS. ME (5 μmol · L−1) pretreatment reduced the negative effects of CS by recovering plant growth, root traits, gas exchange elements, and pigment molecules compared to CS control treatment. Furthermore, ME application efficiently reduced oxidative stress markers [hydrogen peroxide (H2O2), superoxide ion (O2·–), EL, and MDA] while increasing proline and soluble sugar content in pepper leaves. ME application combined with CS further increased antioxidant enzymes and related gene expression. Collectively, our results confirmed the mitigating potential of ME supplementation for CS by maintaining pepper seedling growth, improving the photosynthesis apparatus, regulating pigments, and osmolyte content.http://www.sciencedirect.com/science/article/pii/S2468014123000262PepperMelatoninCold stressAntioxidant enzymeRoot trait |
| spellingShingle | Muhammad Ahsan Altaf Yuanyuan Hao Huangying Shu Weiheng Jin Chuhao Chen Lin Li Yu Zhang Muhammad Ali Mumtaz Huizhen Fu Shanhan Cheng Guopeng Zhu Zhiwei Wang Melatonin mitigates cold-induced damage to pepper seedlings by promoting redox homeostasis and regulating antioxidant profiling Horticultural Plant Journal Pepper Melatonin Cold stress Antioxidant enzyme Root trait |
| title | Melatonin mitigates cold-induced damage to pepper seedlings by promoting redox homeostasis and regulating antioxidant profiling |
| title_full | Melatonin mitigates cold-induced damage to pepper seedlings by promoting redox homeostasis and regulating antioxidant profiling |
| title_fullStr | Melatonin mitigates cold-induced damage to pepper seedlings by promoting redox homeostasis and regulating antioxidant profiling |
| title_full_unstemmed | Melatonin mitigates cold-induced damage to pepper seedlings by promoting redox homeostasis and regulating antioxidant profiling |
| title_short | Melatonin mitigates cold-induced damage to pepper seedlings by promoting redox homeostasis and regulating antioxidant profiling |
| title_sort | melatonin mitigates cold induced damage to pepper seedlings by promoting redox homeostasis and regulating antioxidant profiling |
| topic | Pepper Melatonin Cold stress Antioxidant enzyme Root trait |
| url | http://www.sciencedirect.com/science/article/pii/S2468014123000262 |
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