Identification of DNA G–quadruplex Forming Sequence in Shrimp White Spot Syndrome Virus (WSSV)
White spot syndrome virus (WSSV) is considered one of the most infectious and lethal viruses that affect shrimp. Bioinformatic studies revealed several G-quadruplex forming sequences at the open reading frame region. Moreover, the sequences are widely conserved through all deposited WSSV sequences....
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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EDP Sciences
2023-01-01
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| Series: | E3S Web of Conferences |
| Subjects: | |
| Online Access: | https://www.e3s-conferences.org/articles/e3sconf/pdf/2023/11/e3sconf_3rdnrls2023_00040.pdf |
| _version_ | 1797850842397671424 |
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| author | Maria Vianney Yoanes Kandinata Priscilla Weisz Klaus Goretti Marianti Purwanto Maria |
| author_facet | Maria Vianney Yoanes Kandinata Priscilla Weisz Klaus Goretti Marianti Purwanto Maria |
| author_sort | Maria Vianney Yoanes |
| collection | DOAJ |
| description | White spot syndrome virus (WSSV) is considered one of the most infectious and lethal viruses that affect shrimp. Bioinformatic studies revealed several G-quadruplex forming sequences at the open reading frame region. Moreover, the sequences are widely conserved through all deposited WSSV sequences. Introductory structural studies on two sequences, namely WSSV131 and WSSV172, are proposed to form a quadruplex. While WSSV172 forms a mixture of quadruplex topologies, WSSV131 is suggested to form a parallel topology, as indicated by the NMR spectra and circular dichroism (CD) ellipticity pattern. CD spectra also suggested that the major parallel species of the WSSV131 sequence are found to be stable above 60 °C. Ultimately, these results may open a new strategy for WSSV treatment by targeting the quadruplex confirmation with a quadruplex binding ligand. |
| first_indexed | 2024-04-09T19:08:06Z |
| format | Article |
| id | doaj.art-70f323dc5cfc4051a03611eaac3ea7b5 |
| institution | Directory Open Access Journal |
| issn | 2267-1242 |
| language | English |
| last_indexed | 2024-04-09T19:08:06Z |
| publishDate | 2023-01-01 |
| publisher | EDP Sciences |
| record_format | Article |
| series | E3S Web of Conferences |
| spelling | doaj.art-70f323dc5cfc4051a03611eaac3ea7b52023-04-07T08:56:14ZengEDP SciencesE3S Web of Conferences2267-12422023-01-013740004010.1051/e3sconf/202337400040e3sconf_3rdnrls2023_00040Identification of DNA G–quadruplex Forming Sequence in Shrimp White Spot Syndrome Virus (WSSV)Maria Vianney Yoanes0Kandinata Priscilla1Weisz Klaus2Goretti Marianti Purwanto Maria3Institute of Biochemistry, University of GreifswaldFakultas Teknobiologi, Universitas SurabayaInstitute of Biochemistry, University of GreifswaldFakultas Teknobiologi, Universitas SurabayaWhite spot syndrome virus (WSSV) is considered one of the most infectious and lethal viruses that affect shrimp. Bioinformatic studies revealed several G-quadruplex forming sequences at the open reading frame region. Moreover, the sequences are widely conserved through all deposited WSSV sequences. Introductory structural studies on two sequences, namely WSSV131 and WSSV172, are proposed to form a quadruplex. While WSSV172 forms a mixture of quadruplex topologies, WSSV131 is suggested to form a parallel topology, as indicated by the NMR spectra and circular dichroism (CD) ellipticity pattern. CD spectra also suggested that the major parallel species of the WSSV131 sequence are found to be stable above 60 °C. Ultimately, these results may open a new strategy for WSSV treatment by targeting the quadruplex confirmation with a quadruplex binding ligand.https://www.e3s-conferences.org/articles/e3sconf/pdf/2023/11/e3sconf_3rdnrls2023_00040.pdfbioinformaticsincrease export commoditiesmajor endemic diseaseshrimp farming industry |
| spellingShingle | Maria Vianney Yoanes Kandinata Priscilla Weisz Klaus Goretti Marianti Purwanto Maria Identification of DNA G–quadruplex Forming Sequence in Shrimp White Spot Syndrome Virus (WSSV) E3S Web of Conferences bioinformatics increase export commodities major endemic disease shrimp farming industry |
| title | Identification of DNA G–quadruplex Forming Sequence in Shrimp White Spot Syndrome Virus (WSSV) |
| title_full | Identification of DNA G–quadruplex Forming Sequence in Shrimp White Spot Syndrome Virus (WSSV) |
| title_fullStr | Identification of DNA G–quadruplex Forming Sequence in Shrimp White Spot Syndrome Virus (WSSV) |
| title_full_unstemmed | Identification of DNA G–quadruplex Forming Sequence in Shrimp White Spot Syndrome Virus (WSSV) |
| title_short | Identification of DNA G–quadruplex Forming Sequence in Shrimp White Spot Syndrome Virus (WSSV) |
| title_sort | identification of dna g quadruplex forming sequence in shrimp white spot syndrome virus wssv |
| topic | bioinformatics increase export commodities major endemic disease shrimp farming industry |
| url | https://www.e3s-conferences.org/articles/e3sconf/pdf/2023/11/e3sconf_3rdnrls2023_00040.pdf |
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