Hydroxyapatite-chitosan composites derived from sea cucumbers and shrimp shells ameliorate femoral bone defects in an albino rat model
Background and Aim: A bone defect is defined as a critically sized autologous bone and a bone gap. Bone grafting is one of the most commonly used surgical methods to enhance bone regeneration in orthopedic procedures. A composite of collagen, hydroxyapatite (HA), and chitosan (Ch) is suitable as a b...
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| Format: | Article |
| Language: | English |
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Veterinary World
2023-05-01
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| Series: | Veterinary World |
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| Online Access: | https://www.veterinaryworld.org/Vol.16/May-2023/22.pdf |
| _version_ | 1827940827047919616 |
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| author | Arifia Safira Cinta Atsa Mahesa Rani Faisal Fikri Agus Purnomo Shafia Khairani Shekhar Chhetri Salipudin Tasil Maslamama Muhammad Thohawi Elziyad Purnama |
| author_facet | Arifia Safira Cinta Atsa Mahesa Rani Faisal Fikri Agus Purnomo Shafia Khairani Shekhar Chhetri Salipudin Tasil Maslamama Muhammad Thohawi Elziyad Purnama |
| author_sort | Arifia Safira |
| collection | DOAJ |
| description | Background and Aim: A bone defect is defined as a critically sized autologous bone and a bone gap. Bone grafting is one of the most commonly used surgical methods to enhance bone regeneration in orthopedic procedures. A composite of collagen, hydroxyapatite (HA), and chitosan (Ch) is suitable as a bone matrix and stimulates ossification. This study aimed to evaluate the use of natural HA-Ch composites derived from sea cucumbers and shrimp shells and quantify the levels of cytokines, polymorphonuclear neutrophils (PMNs), serum liver enzymes, calcium, phosphate, and procollagen type 1 N-terminal propeptide (PINP) in albino rats with femoral bone defects.
Materials and Methods: A total of 48 albino rats with femoral bone defects were divided into 4 groups (n = 12 each): (C–) placebo, (C+) polyethylene glycol, (T1) HA, and (T2) HA–Ch groups. Each group was divided into two subgroups (n = 6 each), with euthanization on 7- and 42-day post-treatment, respectively. Procollagen Type 1 N-terminal propeptide and the cytokines interleukin (IL)-4, IL-6, IL-10, and tumor necrosis factor-alpha were quantified using enzyme-linked immunosorbent assay. Flow cytometry was performed to evaluate PMNs. A clinical chemistry analyzer was used to measure the serum levels of liver enzymes, calcium, and phosphate.
Results: There was a significant decrease in the level of IL-6 on 7 days and in the level of IL-10 on 42 days in the HA-Ch group. The level of PMNs also decreased significantly on 7 and 42 days in the HA-Ch group. Regarding serum liver enzymes, alkaline phosphatase (ALP) levels in the HA-Ch group increased significantly on 42 days. Calcium and phosphate levels increased significantly on 7 and 42 days in the HA and HA-Ch groups, and PINP levels increased significantly on 7 and 42 days in the HA-Ch group.
Conclusion: The HA-Ch composite derived from sea cucumbers and shrimp shells ameliorated femoral bone defects in albino rats. The HA-Ch composite modulated the levels of IL-6, IL-10, PMNs, ALP, calcium, phosphate, and PINP on 7- and 42-day post-treatment. |
| first_indexed | 2024-03-13T09:27:14Z |
| format | Article |
| id | doaj.art-70f357e60ac44b6cae9504dd11842f2a |
| institution | Directory Open Access Journal |
| issn | 0972-8988 2231-0916 |
| language | English |
| last_indexed | 2024-03-13T09:27:14Z |
| publishDate | 2023-05-01 |
| publisher | Veterinary World |
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| series | Veterinary World |
| spelling | doaj.art-70f357e60ac44b6cae9504dd11842f2a2023-05-26T08:25:49ZengVeterinary WorldVeterinary World0972-89882231-09162023-05-011651084109110.14202/vetworld.2023.1084-1091Hydroxyapatite-chitosan composites derived from sea cucumbers and shrimp shells ameliorate femoral bone defects in an albino rat modelArifia Safira0https://orcid.org/0000-0001-8931-4126Cinta Atsa Mahesa Rani1https://orcid.org/0000-0001-8771-0723Faisal Fikri2https://orcid.org/0000-0002-7865-427XAgus Purnomo3https://orcid.org/0000-0003-3657-9707Shafia Khairani4https://orcid.org/0000-0002-6225-1388Shekhar Chhetri5https://orcid.org/0000-0003-1984-9509Salipudin Tasil Maslamama6https://orcid.org/0000-0001-7369-0215Muhammad Thohawi Elziyad Purnama7https://orcid.org/0000-0002-9496-0330Department of Veterinary Science, School of Health and Life Sciences, Universitas Airlangga, Surabaya, Indonesia.Department of Veterinary Science, School of Health and Life Sciences, Universitas Airlangga, Surabaya, Indonesia.Department of Veterinary Science, School of Health and Life Sciences, Universitas Airlangga, Surabaya, Indonesia.Department of Veterinary Surgery and Radiology, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia.Department of Biomedical Science, Faculty of Medicine, Universitas Padjajaran, Bandung, Indonesia.Department of Animal Science, College of Natural Resources, Royal University of Bhutan, Lobesa, Punakha, Bhutan.Department of Agricultural Biotechnology, Faculty of Agriculture, Eskişehir Osmangazi Üniversitesi, Eskişehir, Turkey.Department of Veterinary Science, School of Health and Life Sciences, Universitas Airlangga, Surabaya, Indonesia; Department of Biology, Graduate School of Natural and Applied Sciences, Eskişehir Osmangazi Üniversitesi, Eskişehir, Turkey.Background and Aim: A bone defect is defined as a critically sized autologous bone and a bone gap. Bone grafting is one of the most commonly used surgical methods to enhance bone regeneration in orthopedic procedures. A composite of collagen, hydroxyapatite (HA), and chitosan (Ch) is suitable as a bone matrix and stimulates ossification. This study aimed to evaluate the use of natural HA-Ch composites derived from sea cucumbers and shrimp shells and quantify the levels of cytokines, polymorphonuclear neutrophils (PMNs), serum liver enzymes, calcium, phosphate, and procollagen type 1 N-terminal propeptide (PINP) in albino rats with femoral bone defects. Materials and Methods: A total of 48 albino rats with femoral bone defects were divided into 4 groups (n = 12 each): (C–) placebo, (C+) polyethylene glycol, (T1) HA, and (T2) HA–Ch groups. Each group was divided into two subgroups (n = 6 each), with euthanization on 7- and 42-day post-treatment, respectively. Procollagen Type 1 N-terminal propeptide and the cytokines interleukin (IL)-4, IL-6, IL-10, and tumor necrosis factor-alpha were quantified using enzyme-linked immunosorbent assay. Flow cytometry was performed to evaluate PMNs. A clinical chemistry analyzer was used to measure the serum levels of liver enzymes, calcium, and phosphate. Results: There was a significant decrease in the level of IL-6 on 7 days and in the level of IL-10 on 42 days in the HA-Ch group. The level of PMNs also decreased significantly on 7 and 42 days in the HA-Ch group. Regarding serum liver enzymes, alkaline phosphatase (ALP) levels in the HA-Ch group increased significantly on 42 days. Calcium and phosphate levels increased significantly on 7 and 42 days in the HA and HA-Ch groups, and PINP levels increased significantly on 7 and 42 days in the HA-Ch group. Conclusion: The HA-Ch composite derived from sea cucumbers and shrimp shells ameliorated femoral bone defects in albino rats. The HA-Ch composite modulated the levels of IL-6, IL-10, PMNs, ALP, calcium, phosphate, and PINP on 7- and 42-day post-treatment.https://www.veterinaryworld.org/Vol.16/May-2023/22.pdfbone defectchitosanhuman and healthhydroxyapatitesea cucumbershrimp shell |
| spellingShingle | Arifia Safira Cinta Atsa Mahesa Rani Faisal Fikri Agus Purnomo Shafia Khairani Shekhar Chhetri Salipudin Tasil Maslamama Muhammad Thohawi Elziyad Purnama Hydroxyapatite-chitosan composites derived from sea cucumbers and shrimp shells ameliorate femoral bone defects in an albino rat model Veterinary World bone defect chitosan human and health hydroxyapatite sea cucumber shrimp shell |
| title | Hydroxyapatite-chitosan composites derived from sea cucumbers and shrimp shells ameliorate femoral bone defects in an albino rat model |
| title_full | Hydroxyapatite-chitosan composites derived from sea cucumbers and shrimp shells ameliorate femoral bone defects in an albino rat model |
| title_fullStr | Hydroxyapatite-chitosan composites derived from sea cucumbers and shrimp shells ameliorate femoral bone defects in an albino rat model |
| title_full_unstemmed | Hydroxyapatite-chitosan composites derived from sea cucumbers and shrimp shells ameliorate femoral bone defects in an albino rat model |
| title_short | Hydroxyapatite-chitosan composites derived from sea cucumbers and shrimp shells ameliorate femoral bone defects in an albino rat model |
| title_sort | hydroxyapatite chitosan composites derived from sea cucumbers and shrimp shells ameliorate femoral bone defects in an albino rat model |
| topic | bone defect chitosan human and health hydroxyapatite sea cucumber shrimp shell |
| url | https://www.veterinaryworld.org/Vol.16/May-2023/22.pdf |
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