Quantitative proteomics and bioinformatic analysis provide new insight into the dynamic response of porcine intestine to Salmonella Typhimurium.
The enteropathogen Salmonella Typhimurium (S. Typhimurium) is the most commonly nontyphoideal serotype isolated in pig worldwide. Currently, one of the main sources of human infection is by consumption of pork meat. Therefore, prevention and control of salmonellosis in pigs is crucial for minimizing...
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| Format: | Article |
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Frontiers Media S.A.
2015-09-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fcimb.2015.00064/full |
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| author | Melania eCollado-Romero Carmen eAguilar Cristina eArce Concepcion eLucena Marius Cosmin Codrea Luis eMorera Emoke eBendixen Angela eMoreno Angela eMoreno Juan J Garrido |
| author_facet | Melania eCollado-Romero Carmen eAguilar Cristina eArce Concepcion eLucena Marius Cosmin Codrea Luis eMorera Emoke eBendixen Angela eMoreno Angela eMoreno Juan J Garrido |
| author_sort | Melania eCollado-Romero |
| collection | DOAJ |
| description | The enteropathogen Salmonella Typhimurium (S. Typhimurium) is the most commonly nontyphoideal serotype isolated in pig worldwide. Currently, one of the main sources of human infection is by consumption of pork meat. Therefore, prevention and control of salmonellosis in pigs is crucial for minimizing risks to public health. The aim of the present study was to use isobaric tags for relative and absolute quantification (iTRAQ) to explore differences in the response to Salmonella in two segment of the porcine gut (ileum and colon) along a time course of 1, 2 and 6 days post infection (dpi) with S. Typhimurium. A total of 298 proteins were identified in the infected ileum samples of which, 112 displayed significant expression differences due to Salmonella infection. In colon, 184 proteins were detected in the infected samples of which 46 resulted differentially expressed with respect to the controls. The higher number of changes in protein expression was quantified in ileum at 2 dpi. Further biological interpretation of proteomics data using bioinformatics tools demonstrated that the expression changes in colon were found in proteins involved in cell death and survival, tissue morphology or molecular transport at the early stages and tissue regeneration at 6 dpi. In ileum, however, changes in protein expression were mainly related to immunological and infection diseases, inflammatory response or connective tissue disorders at 1 and 2 dpi. iTRAQ has proved to be a proteomic robust approach allowing us to identify ileum as the earliest response focus upon S. Typhimurium in the porcine gut. In addition, new functions involved in the response to bacteria such as eIF2 signalling, free radical scavengers or antimicrobial peptides expression have been identified. Finally, the impairment at of the enterohepatic circulation of bile acids and lipid metabolism by means the under regulation of FABP6 protein and FXR/RXR and LXR/RXR signalling pathway in ileum has been established for the first time in pigs. Taken together, our results provide a better understanding of the porcine response to Salmonella infection and the molecular mechanisms underlying Salmonella-host interactions. |
| first_indexed | 2024-12-11T08:31:22Z |
| format | Article |
| id | doaj.art-70f719d3560c4aed94cac8d62a920cba |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-12-11T08:31:22Z |
| publishDate | 2015-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-70f719d3560c4aed94cac8d62a920cba2022-12-22T01:14:28ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882015-09-01510.3389/fcimb.2015.00064150915Quantitative proteomics and bioinformatic analysis provide new insight into the dynamic response of porcine intestine to Salmonella Typhimurium.Melania eCollado-Romero0Carmen eAguilar1Cristina eArce2Concepcion eLucena3Marius Cosmin Codrea4Luis eMorera5Emoke eBendixen6Angela eMoreno7Angela eMoreno8Juan J Garrido9University of CordobaUniversity of CordobaUniversity of CordobaUniversity of CordobaAarhus UniversityUniversity of CordobaAarhus UniversityUniversity of CordobaInstitute for Sustainable Agriculture CSICUniversity of CordobaThe enteropathogen Salmonella Typhimurium (S. Typhimurium) is the most commonly nontyphoideal serotype isolated in pig worldwide. Currently, one of the main sources of human infection is by consumption of pork meat. Therefore, prevention and control of salmonellosis in pigs is crucial for minimizing risks to public health. The aim of the present study was to use isobaric tags for relative and absolute quantification (iTRAQ) to explore differences in the response to Salmonella in two segment of the porcine gut (ileum and colon) along a time course of 1, 2 and 6 days post infection (dpi) with S. Typhimurium. A total of 298 proteins were identified in the infected ileum samples of which, 112 displayed significant expression differences due to Salmonella infection. In colon, 184 proteins were detected in the infected samples of which 46 resulted differentially expressed with respect to the controls. The higher number of changes in protein expression was quantified in ileum at 2 dpi. Further biological interpretation of proteomics data using bioinformatics tools demonstrated that the expression changes in colon were found in proteins involved in cell death and survival, tissue morphology or molecular transport at the early stages and tissue regeneration at 6 dpi. In ileum, however, changes in protein expression were mainly related to immunological and infection diseases, inflammatory response or connective tissue disorders at 1 and 2 dpi. iTRAQ has proved to be a proteomic robust approach allowing us to identify ileum as the earliest response focus upon S. Typhimurium in the porcine gut. In addition, new functions involved in the response to bacteria such as eIF2 signalling, free radical scavengers or antimicrobial peptides expression have been identified. Finally, the impairment at of the enterohepatic circulation of bile acids and lipid metabolism by means the under regulation of FABP6 protein and FXR/RXR and LXR/RXR signalling pathway in ileum has been established for the first time in pigs. Taken together, our results provide a better understanding of the porcine response to Salmonella infection and the molecular mechanisms underlying Salmonella-host interactions.http://journal.frontiersin.org/Journal/10.3389/fcimb.2015.00064/fullColonIleumSalmonella typhimuriumiTRAQIntestinal responseexperimentally infected pigs |
| spellingShingle | Melania eCollado-Romero Carmen eAguilar Cristina eArce Concepcion eLucena Marius Cosmin Codrea Luis eMorera Emoke eBendixen Angela eMoreno Angela eMoreno Juan J Garrido Quantitative proteomics and bioinformatic analysis provide new insight into the dynamic response of porcine intestine to Salmonella Typhimurium. Frontiers in Cellular and Infection Microbiology Colon Ileum Salmonella typhimurium iTRAQ Intestinal response experimentally infected pigs |
| title | Quantitative proteomics and bioinformatic analysis provide new insight into the dynamic response of porcine intestine to Salmonella Typhimurium. |
| title_full | Quantitative proteomics and bioinformatic analysis provide new insight into the dynamic response of porcine intestine to Salmonella Typhimurium. |
| title_fullStr | Quantitative proteomics and bioinformatic analysis provide new insight into the dynamic response of porcine intestine to Salmonella Typhimurium. |
| title_full_unstemmed | Quantitative proteomics and bioinformatic analysis provide new insight into the dynamic response of porcine intestine to Salmonella Typhimurium. |
| title_short | Quantitative proteomics and bioinformatic analysis provide new insight into the dynamic response of porcine intestine to Salmonella Typhimurium. |
| title_sort | quantitative proteomics and bioinformatic analysis provide new insight into the dynamic response of porcine intestine to salmonella typhimurium |
| topic | Colon Ileum Salmonella typhimurium iTRAQ Intestinal response experimentally infected pigs |
| url | http://journal.frontiersin.org/Journal/10.3389/fcimb.2015.00064/full |
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