Milk Fat Globule Membrane Proteomics: A 'Snapshot' of Mammary Epithelial Cell Biology
Lipids are released in milk as fat globules, which are droplets of apolar lipids surrounded by a complex membrane deriving from the mammary epithelial cell (MEC) and called the milk fat globule membrane (MFGM). The structure of the MFGM is highly complex and closely related to the mechanisms of milk...
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| Format: | Article |
| Language: | English |
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University of Zagreb Faculty of Food Technology and Biotechnology
2012-01-01
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| Series: | Food Technology and Biotechnology |
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| Online Access: | http://hrcak.srce.hr/file/129384 |
| _version_ | 1797424687217639424 |
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| author | Christelle Cebo |
| author_facet | Christelle Cebo |
| author_sort | Christelle Cebo |
| collection | DOAJ |
| description | Lipids are released in milk as fat globules, which are droplets of apolar lipids surrounded by a complex membrane deriving from the mammary epithelial cell (MEC) and called the milk fat globule membrane (MFGM). The structure of the MFGM is highly complex and closely related to the mechanisms of milk fat globule secretion in the mammary epithelial cell. Indeed, MFGM is composed of two biological membranes, a phospholipid monolayer, deriving from the endoplasmic reticulum, and a phospholipid bilayer, which originates from the apical plasma membrane of the MEC, with variable amounts of cytoplasm trapped between. Biochemical techniques (i.e. sodium dodecyl sulphate-polyacrylamide gel electrophoresis followed by different staining procedures) have been employed historically to characterize major MFGM proteins, namely MUC-1, fatty acid synthase, xanthine oxidase, butyrophilin, lactadherin, and adipophilin. However, recent advances in the field of proteomics (mostly development of one-dimensional gel electrophoresis approach coupled with tandem mass spectrometry) have led to the identification of hundreds of proteins associated with the MFGM. Surprisingly, newly identified MFGM proteins were not only involved in lipid metabolic or exocytosis-related biological processes, but also in cell signalling, translation, or host defense-related mechanisms. Therefore, the milk fat globule should no longer be viewed as an inert structure only devoted to the delivery of lipids to the newborn, but rather as a dynamic and informative compartment which can contribute to the improvement of our comprehension of the mammary gland biology. |
| first_indexed | 2024-03-09T08:05:31Z |
| format | Article |
| id | doaj.art-70f8a309476d4f72baf3e4d6ac798496 |
| institution | Directory Open Access Journal |
| issn | 1330-9862 1334-2606 |
| language | English |
| last_indexed | 2024-03-09T08:05:31Z |
| publishDate | 2012-01-01 |
| publisher | University of Zagreb Faculty of Food Technology and Biotechnology |
| record_format | Article |
| series | Food Technology and Biotechnology |
| spelling | doaj.art-70f8a309476d4f72baf3e4d6ac7984962023-12-03T00:19:02ZengUniversity of Zagreb Faculty of Food Technology and BiotechnologyFood Technology and Biotechnology1330-98621334-26062012-01-01503306314Milk Fat Globule Membrane Proteomics: A 'Snapshot' of Mammary Epithelial Cell BiologyChristelle Cebo0National Institute of Agronomy Research (INRA), UMR1313 Animal Genetics and Integrative Biology, FR-78350 Jouy-en-Josas, FranceLipids are released in milk as fat globules, which are droplets of apolar lipids surrounded by a complex membrane deriving from the mammary epithelial cell (MEC) and called the milk fat globule membrane (MFGM). The structure of the MFGM is highly complex and closely related to the mechanisms of milk fat globule secretion in the mammary epithelial cell. Indeed, MFGM is composed of two biological membranes, a phospholipid monolayer, deriving from the endoplasmic reticulum, and a phospholipid bilayer, which originates from the apical plasma membrane of the MEC, with variable amounts of cytoplasm trapped between. Biochemical techniques (i.e. sodium dodecyl sulphate-polyacrylamide gel electrophoresis followed by different staining procedures) have been employed historically to characterize major MFGM proteins, namely MUC-1, fatty acid synthase, xanthine oxidase, butyrophilin, lactadherin, and adipophilin. However, recent advances in the field of proteomics (mostly development of one-dimensional gel electrophoresis approach coupled with tandem mass spectrometry) have led to the identification of hundreds of proteins associated with the MFGM. Surprisingly, newly identified MFGM proteins were not only involved in lipid metabolic or exocytosis-related biological processes, but also in cell signalling, translation, or host defense-related mechanisms. Therefore, the milk fat globule should no longer be viewed as an inert structure only devoted to the delivery of lipids to the newborn, but rather as a dynamic and informative compartment which can contribute to the improvement of our comprehension of the mammary gland biology.http://hrcak.srce.hr/file/129384milk fat globule membranemass spectrometry-based proteomicsmammary gland biologyintegrative biology |
| spellingShingle | Christelle Cebo Milk Fat Globule Membrane Proteomics: A 'Snapshot' of Mammary Epithelial Cell Biology Food Technology and Biotechnology milk fat globule membrane mass spectrometry-based proteomics mammary gland biology integrative biology |
| title | Milk Fat Globule Membrane Proteomics: A 'Snapshot' of Mammary Epithelial Cell Biology |
| title_full | Milk Fat Globule Membrane Proteomics: A 'Snapshot' of Mammary Epithelial Cell Biology |
| title_fullStr | Milk Fat Globule Membrane Proteomics: A 'Snapshot' of Mammary Epithelial Cell Biology |
| title_full_unstemmed | Milk Fat Globule Membrane Proteomics: A 'Snapshot' of Mammary Epithelial Cell Biology |
| title_short | Milk Fat Globule Membrane Proteomics: A 'Snapshot' of Mammary Epithelial Cell Biology |
| title_sort | milk fat globule membrane proteomics a snapshot of mammary epithelial cell biology |
| topic | milk fat globule membrane mass spectrometry-based proteomics mammary gland biology integrative biology |
| url | http://hrcak.srce.hr/file/129384 |
| work_keys_str_mv | AT christellecebo milkfatglobulemembraneproteomicsasnapshotofmammaryepithelialcellbiology |