Terahertz refractive phenotype of living cells
Cellular refractive index is a vital phenotypic parameter for understanding the cell functional activities. So far, there remains technical challenges to obtain refractive index of viable cells at the terahertz frequency in which contains rich information closely related to their physiological statu...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-01-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2022.1105249/full |
| _version_ | 1797956524133318656 |
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| author | Guangxu Zhang Guangxu Zhang Guangxu Zhang Yadi Wang Yadi Wang Jiang Qian Jiang Qian Jiang Qian Yue Wang Yue Wang Yue Wang Xueling Li Xueling Li Junhong Lü Junhong Lü Junhong Lü Junhong Lü Junhong Lü |
| author_facet | Guangxu Zhang Guangxu Zhang Guangxu Zhang Yadi Wang Yadi Wang Jiang Qian Jiang Qian Jiang Qian Yue Wang Yue Wang Yue Wang Xueling Li Xueling Li Junhong Lü Junhong Lü Junhong Lü Junhong Lü Junhong Lü |
| author_sort | Guangxu Zhang |
| collection | DOAJ |
| description | Cellular refractive index is a vital phenotypic parameter for understanding the cell functional activities. So far, there remains technical challenges to obtain refractive index of viable cells at the terahertz frequency in which contains rich information closely related to their physiological status. Here we introduce a label-free optical platform for interrogating cellular phenotypes to measure the refractive index of living cells in near-physiological environments by using terahertz spectroscopy with the combination of cellular encapsulation in a confined solution droplet. The key technical feature with cells encapsulated in aqueous droplets allows for keeping cellular viability while eliminating the strong adsorption of solvent water to terahertz signal. The obtained high signal-to-noise ratio enables to differentiate different cell types (e.g., E. coli, stem cell and cancer cell) and their states under stress conditions. The integrating of terahertz spectroscopy to droplet microfluidic further realizes automated and high-through sample preparation and detection, providing a practical toolkit for potential application in cellular health evaluation and phenotypic drug discovery. |
| first_indexed | 2024-04-10T23:50:13Z |
| format | Article |
| id | doaj.art-70f9816b6923400f985d79dc4f9392ae |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-04-10T23:50:13Z |
| publishDate | 2023-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-70f9816b6923400f985d79dc4f9392ae2023-01-10T18:48:19ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852023-01-011010.3389/fbioe.2022.11052491105249Terahertz refractive phenotype of living cellsGuangxu Zhang0Guangxu Zhang1Guangxu Zhang2Yadi Wang3Yadi Wang4Jiang Qian5Jiang Qian6Jiang Qian7Yue Wang8Yue Wang9Yue Wang10Xueling Li11Xueling Li12Junhong Lü13Junhong Lü14Junhong Lü15Junhong Lü16Junhong Lü17Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai, ChinaJinan Microecological Biomedicine Shandong Laboratory, Jinan, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaJinan Microecological Biomedicine Shandong Laboratory, Jinan, ChinaSchool of Pharmacy, Binzhou Medical University, Yantai, ChinaShanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai, ChinaJinan Microecological Biomedicine Shandong Laboratory, Jinan, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaShanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai, ChinaJinan Microecological Biomedicine Shandong Laboratory, Jinan, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaJinan Microecological Biomedicine Shandong Laboratory, Jinan, ChinaShanghai University of Medicine and Health Sciences, Shanghai, ChinaShanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai, ChinaJinan Microecological Biomedicine Shandong Laboratory, Jinan, ChinaSchool of Pharmacy, Binzhou Medical University, Yantai, ChinaShanghai University of Medicine and Health Sciences, Shanghai, ChinaShanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai, ChinaCellular refractive index is a vital phenotypic parameter for understanding the cell functional activities. So far, there remains technical challenges to obtain refractive index of viable cells at the terahertz frequency in which contains rich information closely related to their physiological status. Here we introduce a label-free optical platform for interrogating cellular phenotypes to measure the refractive index of living cells in near-physiological environments by using terahertz spectroscopy with the combination of cellular encapsulation in a confined solution droplet. The key technical feature with cells encapsulated in aqueous droplets allows for keeping cellular viability while eliminating the strong adsorption of solvent water to terahertz signal. The obtained high signal-to-noise ratio enables to differentiate different cell types (e.g., E. coli, stem cell and cancer cell) and their states under stress conditions. The integrating of terahertz spectroscopy to droplet microfluidic further realizes automated and high-through sample preparation and detection, providing a practical toolkit for potential application in cellular health evaluation and phenotypic drug discovery.https://www.frontiersin.org/articles/10.3389/fbioe.2022.1105249/fullterahertz spectroscopyrefractive indexwater dropletliving cellsdroplet microfluidic |
| spellingShingle | Guangxu Zhang Guangxu Zhang Guangxu Zhang Yadi Wang Yadi Wang Jiang Qian Jiang Qian Jiang Qian Yue Wang Yue Wang Yue Wang Xueling Li Xueling Li Junhong Lü Junhong Lü Junhong Lü Junhong Lü Junhong Lü Terahertz refractive phenotype of living cells Frontiers in Bioengineering and Biotechnology terahertz spectroscopy refractive index water droplet living cells droplet microfluidic |
| title | Terahertz refractive phenotype of living cells |
| title_full | Terahertz refractive phenotype of living cells |
| title_fullStr | Terahertz refractive phenotype of living cells |
| title_full_unstemmed | Terahertz refractive phenotype of living cells |
| title_short | Terahertz refractive phenotype of living cells |
| title_sort | terahertz refractive phenotype of living cells |
| topic | terahertz spectroscopy refractive index water droplet living cells droplet microfluidic |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2022.1105249/full |
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