Akkermansia Muciniphila Suppresses Non-alcoholic Steatohepatitis-associated Liver Cancer in Mice by Recovering Intestinal Barrier Function
Objective To investigate whether the next-generation probiotics Akkermansia muciniphila can prevent non-alcoholic steatohepatitis (NASH)-related hepatocellular carcinoma (HCC). Methods We constructed a NASH-HCC model called STAM. STAM mice received oral saline or A. muciniphila starting at 4 weeks o...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | zho |
| Published: |
Magazine House of Cancer Research on Prevention and Treatment
2023-05-01
|
| Series: | Zhongliu Fangzhi Yanjiu |
| Subjects: | |
| Online Access: | http://www.zlfzyj.com/EN/10.3971/j.issn.1000-8578.2023.22.1259 |
| _version_ | 1797808885227061248 |
|---|---|
| author | ZHANG Wujian LI Tao |
| author_facet | ZHANG Wujian LI Tao |
| author_sort | ZHANG Wujian |
| collection | DOAJ |
| description | Objective To investigate whether the next-generation probiotics Akkermansia muciniphila can prevent non-alcoholic steatohepatitis (NASH)-related hepatocellular carcinoma (HCC). Methods We constructed a NASH-HCC model called STAM. STAM mice received oral saline or A. muciniphila starting at 4 weeks of age. Liver tissues were evaluated by HE staining and oil red O staining for NASH activity, and intrahepatic expression levels of inflammatory cytokines and ileal tight junction proteins were measured by RT-PCR. Results At 8 weeks of age, the steatosis, ballooning degeneration and NAS scores, TNF-α, MCP-1, IL-1β, and IL-6 mRNA expression were significantly decreased in the STAM+A. muciniphila group (both P < 0.05) compared with those in the STAM+saline group (all P < 0.05). At 20 weeks of age, the number of liver surface tumors formed, tumor size and IL-6 level were decreased in the STAM + A. muciniphila group (all P < 0.05). A. muciniphila restored the thickness of the colon mucosal layer and the number of goblet cells in STAM mice as well as increased the mRNA expression of the tight junction proteins ZO-1, Claudin-3, and Occludin in ileal epithelial cells. Conclusion Akkermansia muciniphila can inhibit the progression of NASH to HCC by improving the intestinal barrier function and may serve as a candidate drug to prevent NASH-HCC. |
| first_indexed | 2024-03-13T06:44:18Z |
| format | Article |
| id | doaj.art-70fa5f0bbe2a4a7ba0f7c0c18f6c1389 |
| institution | Directory Open Access Journal |
| issn | 1000-8578 |
| language | zho |
| last_indexed | 2024-03-13T06:44:18Z |
| publishDate | 2023-05-01 |
| publisher | Magazine House of Cancer Research on Prevention and Treatment |
| record_format | Article |
| series | Zhongliu Fangzhi Yanjiu |
| spelling | doaj.art-70fa5f0bbe2a4a7ba0f7c0c18f6c13892023-06-08T10:42:08ZzhoMagazine House of Cancer Research on Prevention and TreatmentZhongliu Fangzhi Yanjiu1000-85782023-05-0150546346910.3971/j.issn.1000-8578.2023.22.12598578.2023.22.1259Akkermansia Muciniphila Suppresses Non-alcoholic Steatohepatitis-associated Liver Cancer in Mice by Recovering Intestinal Barrier FunctionZHANG Wujian0LI Tao1Department of Anus-intestines, The First Affiliated Hospital of Heilongjiang Traditional Chinese Medicine, Harbin 150001, ChinaDepartment of Gastroenterology, Nanfang Hospital of Southern Medical University, Guangzhou 510000, ChinaObjective To investigate whether the next-generation probiotics Akkermansia muciniphila can prevent non-alcoholic steatohepatitis (NASH)-related hepatocellular carcinoma (HCC). Methods We constructed a NASH-HCC model called STAM. STAM mice received oral saline or A. muciniphila starting at 4 weeks of age. Liver tissues were evaluated by HE staining and oil red O staining for NASH activity, and intrahepatic expression levels of inflammatory cytokines and ileal tight junction proteins were measured by RT-PCR. Results At 8 weeks of age, the steatosis, ballooning degeneration and NAS scores, TNF-α, MCP-1, IL-1β, and IL-6 mRNA expression were significantly decreased in the STAM+A. muciniphila group (both P < 0.05) compared with those in the STAM+saline group (all P < 0.05). At 20 weeks of age, the number of liver surface tumors formed, tumor size and IL-6 level were decreased in the STAM + A. muciniphila group (all P < 0.05). A. muciniphila restored the thickness of the colon mucosal layer and the number of goblet cells in STAM mice as well as increased the mRNA expression of the tight junction proteins ZO-1, Claudin-3, and Occludin in ileal epithelial cells. Conclusion Akkermansia muciniphila can inhibit the progression of NASH to HCC by improving the intestinal barrier function and may serve as a candidate drug to prevent NASH-HCC.http://www.zlfzyj.com/EN/10.3971/j.issn.1000-8578.2023.22.1259probioticsakkermansia muciniphilanon-alcoholic fatty liver diseasehepatocellular carcinoma |
| spellingShingle | ZHANG Wujian LI Tao Akkermansia Muciniphila Suppresses Non-alcoholic Steatohepatitis-associated Liver Cancer in Mice by Recovering Intestinal Barrier Function Zhongliu Fangzhi Yanjiu probiotics akkermansia muciniphila non-alcoholic fatty liver disease hepatocellular carcinoma |
| title | Akkermansia Muciniphila Suppresses Non-alcoholic Steatohepatitis-associated Liver Cancer in Mice by Recovering Intestinal Barrier Function |
| title_full | Akkermansia Muciniphila Suppresses Non-alcoholic Steatohepatitis-associated Liver Cancer in Mice by Recovering Intestinal Barrier Function |
| title_fullStr | Akkermansia Muciniphila Suppresses Non-alcoholic Steatohepatitis-associated Liver Cancer in Mice by Recovering Intestinal Barrier Function |
| title_full_unstemmed | Akkermansia Muciniphila Suppresses Non-alcoholic Steatohepatitis-associated Liver Cancer in Mice by Recovering Intestinal Barrier Function |
| title_short | Akkermansia Muciniphila Suppresses Non-alcoholic Steatohepatitis-associated Liver Cancer in Mice by Recovering Intestinal Barrier Function |
| title_sort | akkermansia muciniphila suppresses non alcoholic steatohepatitis associated liver cancer in mice by recovering intestinal barrier function |
| topic | probiotics akkermansia muciniphila non-alcoholic fatty liver disease hepatocellular carcinoma |
| url | http://www.zlfzyj.com/EN/10.3971/j.issn.1000-8578.2023.22.1259 |
| work_keys_str_mv | AT zhangwujian akkermansiamuciniphilasuppressesnonalcoholicsteatohepatitisassociatedlivercancerinmicebyrecoveringintestinalbarrierfunction AT litao akkermansiamuciniphilasuppressesnonalcoholicsteatohepatitisassociatedlivercancerinmicebyrecoveringintestinalbarrierfunction |