Paralytic Shellfish Toxins and Cyanotoxins in the Mediterranean: New Data from Sardinia and Sicily (Italy)
Harmful algal blooms represent a severe issue worldwide. They affect ecosystem functions and related services and goods, with consequences on human health and socio-economic activities. This study reports new data on paralytic shellfish toxins (PSTs) from Sardinia and Sicily (Italy), the largest Med...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2017-11-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/5/4/72 |
| _version_ | 1818380667903279104 |
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| author | Antonella Lugliè Maria Grazia Giacobbe Elena Riccardi Milena Bruno Silvia Pigozzi Maria Antonietta Mariani Cecilia Teodora Satta Daniela Stacca Anna Maria Bazzoni Tiziana Caddeo Pasqualina Farina Bachisio Mario Padedda Silvia Pulina Nicola Sechi Anna Milandri |
| author_facet | Antonella Lugliè Maria Grazia Giacobbe Elena Riccardi Milena Bruno Silvia Pigozzi Maria Antonietta Mariani Cecilia Teodora Satta Daniela Stacca Anna Maria Bazzoni Tiziana Caddeo Pasqualina Farina Bachisio Mario Padedda Silvia Pulina Nicola Sechi Anna Milandri |
| author_sort | Antonella Lugliè |
| collection | DOAJ |
| description | Harmful algal blooms represent a severe issue worldwide. They affect ecosystem functions and related services and goods, with consequences on human health and socio-economic activities. This study reports new data on paralytic shellfish toxins (PSTs) from Sardinia and Sicily (Italy), the largest Mediterranean islands where toxic events, mainly caused by Alexandrium species (Dinophyceae), have been ascertained in mussel farms since the 2000s. The toxicity of the A. minutum, A. tamarense and A. pacificum strains, established from the isolation of vegetative cells and resting cysts, was determined by high performance liquid chromatography (HPLC). The analyses indicated the highest toxicity for A. pacificum strains (total PSTs up to 17.811 fmol cell−1). The PSTs were also assessed in a strain of A. tamarense. The results encourage further investigation to increase the knowledge of toxic species still debated in the Mediterranean. This study also reports new data on microcystins (MCs) and β-N-methylamino-L-alanine (BMAA) from a Sardinian artificial lake (Lake Bidighinzu). The presence of MCs and BMAA was assessed in natural samples and in cell cultures by enzyme-linked immunosorbent assay (ELISA). BMAA positives were found in all the analysed samples with a maximum of 17.84 µg L−1. The obtained results added further information on cyanotoxins in Mediterranean reservoirs, particularly BMAA, which have not yet been thoroughly investigated. |
| first_indexed | 2024-12-14T02:22:20Z |
| format | Article |
| id | doaj.art-70fac7b60fec4aa489262b34c81a88e2 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-12-14T02:22:20Z |
| publishDate | 2017-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-70fac7b60fec4aa489262b34c81a88e22022-12-21T23:20:28ZengMDPI AGMicroorganisms2076-26072017-11-01547210.3390/microorganisms5040072microorganisms5040072Paralytic Shellfish Toxins and Cyanotoxins in the Mediterranean: New Data from Sardinia and Sicily (Italy)Antonella Lugliè0Maria Grazia Giacobbe1Elena Riccardi2Milena Bruno3Silvia Pigozzi4Maria Antonietta Mariani5Cecilia Teodora Satta6Daniela Stacca7Anna Maria Bazzoni8Tiziana Caddeo9Pasqualina Farina10Bachisio Mario Padedda11Silvia Pulina12Nicola Sechi13Anna Milandri14Dipartimento di Architettura, Design e Urbanistica, University of Sassari, Via Piandanna 4, 07100 Sassari, ItalyIstituto per l’Ambiente Marino Costiero, CNR, Spianata S. Raineri 86, 98122 Messina, ItalyFondazione Centro Ricerche Marine, National Reference Laboratory for Marine Biotoxins, Viale A. Vespucci 2, 47042 Cesenatico (FC), ItalyEnvironmental Quality and Fish Farming, Environment and Primary Prevention, Istituto Superiore di Sanità, V.le Regina Elena 299, 00161 Rome, ItalyFondazione Centro Ricerche Marine, National Reference Laboratory for Marine Biotoxins, Viale A. Vespucci 2, 47042 Cesenatico (FC), ItalyDipartimento di Architettura, Design e Urbanistica, University of Sassari, Via Piandanna 4, 07100 Sassari, ItalyDipartimento di Architettura, Design e Urbanistica, University of Sassari, Via Piandanna 4, 07100 Sassari, ItalyDipartimento di Architettura, Design e Urbanistica, University of Sassari, Via Piandanna 4, 07100 Sassari, ItalyDipartimento di Ispezione degli Alimenti, Istituto Zooprofilattico Sperimentale della Sardegna G. Pegreffi, Via Duca degli Abruzzi 8, 07100 Sassari, ItalyDipartimento di Architettura, Design e Urbanistica, University of Sassari, Via Piandanna 4, 07100 Sassari, ItalyDipartimento di Architettura, Design e Urbanistica, University of Sassari, Via Piandanna 4, 07100 Sassari, ItalyDipartimento di Architettura, Design e Urbanistica, University of Sassari, Via Piandanna 4, 07100 Sassari, ItalyDipartimento di Architettura, Design e Urbanistica, University of Sassari, Via Piandanna 4, 07100 Sassari, ItalyDipartimento di Architettura, Design e Urbanistica, University of Sassari, Via Piandanna 4, 07100 Sassari, ItalyFondazione Centro Ricerche Marine, National Reference Laboratory for Marine Biotoxins, Viale A. Vespucci 2, 47042 Cesenatico (FC), ItalyHarmful algal blooms represent a severe issue worldwide. They affect ecosystem functions and related services and goods, with consequences on human health and socio-economic activities. This study reports new data on paralytic shellfish toxins (PSTs) from Sardinia and Sicily (Italy), the largest Mediterranean islands where toxic events, mainly caused by Alexandrium species (Dinophyceae), have been ascertained in mussel farms since the 2000s. The toxicity of the A. minutum, A. tamarense and A. pacificum strains, established from the isolation of vegetative cells and resting cysts, was determined by high performance liquid chromatography (HPLC). The analyses indicated the highest toxicity for A. pacificum strains (total PSTs up to 17.811 fmol cell−1). The PSTs were also assessed in a strain of A. tamarense. The results encourage further investigation to increase the knowledge of toxic species still debated in the Mediterranean. This study also reports new data on microcystins (MCs) and β-N-methylamino-L-alanine (BMAA) from a Sardinian artificial lake (Lake Bidighinzu). The presence of MCs and BMAA was assessed in natural samples and in cell cultures by enzyme-linked immunosorbent assay (ELISA). BMAA positives were found in all the analysed samples with a maximum of 17.84 µg L−1. The obtained results added further information on cyanotoxins in Mediterranean reservoirs, particularly BMAA, which have not yet been thoroughly investigated.https://www.mdpi.com/2076-2607/5/4/72paralytic shellfish toxinsmicrocystinsBMAAAlexandriumartificial lakesMediterranean |
| spellingShingle | Antonella Lugliè Maria Grazia Giacobbe Elena Riccardi Milena Bruno Silvia Pigozzi Maria Antonietta Mariani Cecilia Teodora Satta Daniela Stacca Anna Maria Bazzoni Tiziana Caddeo Pasqualina Farina Bachisio Mario Padedda Silvia Pulina Nicola Sechi Anna Milandri Paralytic Shellfish Toxins and Cyanotoxins in the Mediterranean: New Data from Sardinia and Sicily (Italy) Microorganisms paralytic shellfish toxins microcystins BMAA Alexandrium artificial lakes Mediterranean |
| title | Paralytic Shellfish Toxins and Cyanotoxins in the Mediterranean: New Data from Sardinia and Sicily (Italy) |
| title_full | Paralytic Shellfish Toxins and Cyanotoxins in the Mediterranean: New Data from Sardinia and Sicily (Italy) |
| title_fullStr | Paralytic Shellfish Toxins and Cyanotoxins in the Mediterranean: New Data from Sardinia and Sicily (Italy) |
| title_full_unstemmed | Paralytic Shellfish Toxins and Cyanotoxins in the Mediterranean: New Data from Sardinia and Sicily (Italy) |
| title_short | Paralytic Shellfish Toxins and Cyanotoxins in the Mediterranean: New Data from Sardinia and Sicily (Italy) |
| title_sort | paralytic shellfish toxins and cyanotoxins in the mediterranean new data from sardinia and sicily italy |
| topic | paralytic shellfish toxins microcystins BMAA Alexandrium artificial lakes Mediterranean |
| url | https://www.mdpi.com/2076-2607/5/4/72 |
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