Optical EUS Activation to Relax Sensitized Micturition Response
This study aims to activate the external urethral sphincter (EUS), which plays a critical role in micturition control, through optogenetics and to determine its potential contribution to the stabilization of sensitized micturition activity. The viral vector (<i>AAV2/8-CMV-hChR2(H134R)-EGFP<...
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| Format: | Article |
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MDPI AG
2023-09-01
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| Series: | Life |
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| Online Access: | https://www.mdpi.com/2075-1729/13/10/1961 |
| _version_ | 1827720611085942784 |
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| author | Jin-Ki Hong Hyuk-June Moon Hyun-Joon Shin |
| author_facet | Jin-Ki Hong Hyuk-June Moon Hyun-Joon Shin |
| author_sort | Jin-Ki Hong |
| collection | DOAJ |
| description | This study aims to activate the external urethral sphincter (EUS), which plays a critical role in micturition control, through optogenetics and to determine its potential contribution to the stabilization of sensitized micturition activity. The viral vector (<i>AAV2/8-CMV-hChR2(H134R)-EGFP</i>) is utilized to introduce light-gated ion channels (hChR2/H134R) into the EUS of wild-type <i>C57BL/6</i> mice. Following the induction of sensitized micturition activity using weak acetic acid (0.1%) in anesthetized mice, optical stimulation of the EUS muscle tissue expressing channel rhodopsin is performed using a 473 nm laser light delivered through optical fibers, and the resulting changes in muscle activation and micturition activity are examined. Through EMG (electromyography) measurements, it is confirmed that optical stimulation electrically activates the EUS muscle in mice. Analysis of micturition activity using cystometry reveals a 70.58% decrease in the micturition period and a 70.27% decrease in the voiding volume due to sensitized voiding. However, with optical stimulation, the micturition period recovers to 101.49%, and the voiding volume recovered to 100.22%. Stimulation of the EUS using optogenetics can alleviate sensitized micturition activity and holds potential for application in conjunction with other micturition control methods. |
| first_indexed | 2024-03-10T21:06:55Z |
| format | Article |
| id | doaj.art-70fb9b93012c4cbca358368d1fccf64a |
| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-10T21:06:55Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj.art-70fb9b93012c4cbca358368d1fccf64a2023-11-19T17:05:24ZengMDPI AGLife2075-17292023-09-011310196110.3390/life13101961Optical EUS Activation to Relax Sensitized Micturition ResponseJin-Ki Hong0Hyuk-June Moon1Hyun-Joon Shin2Bionics Research Center, Korea Institute of Science and Technology, Seoul 02792, Republic of KoreaBionics Research Center, Korea Institute of Science and Technology, Seoul 02792, Republic of KoreaBionics Research Center, Korea Institute of Science and Technology, Seoul 02792, Republic of KoreaThis study aims to activate the external urethral sphincter (EUS), which plays a critical role in micturition control, through optogenetics and to determine its potential contribution to the stabilization of sensitized micturition activity. The viral vector (<i>AAV2/8-CMV-hChR2(H134R)-EGFP</i>) is utilized to introduce light-gated ion channels (hChR2/H134R) into the EUS of wild-type <i>C57BL/6</i> mice. Following the induction of sensitized micturition activity using weak acetic acid (0.1%) in anesthetized mice, optical stimulation of the EUS muscle tissue expressing channel rhodopsin is performed using a 473 nm laser light delivered through optical fibers, and the resulting changes in muscle activation and micturition activity are examined. Through EMG (electromyography) measurements, it is confirmed that optical stimulation electrically activates the EUS muscle in mice. Analysis of micturition activity using cystometry reveals a 70.58% decrease in the micturition period and a 70.27% decrease in the voiding volume due to sensitized voiding. However, with optical stimulation, the micturition period recovers to 101.49%, and the voiding volume recovered to 100.22%. Stimulation of the EUS using optogenetics can alleviate sensitized micturition activity and holds potential for application in conjunction with other micturition control methods.https://www.mdpi.com/2075-1729/13/10/1961external urethral sphincterstriated muscleoptogeneticsmicturitioncontinence |
| spellingShingle | Jin-Ki Hong Hyuk-June Moon Hyun-Joon Shin Optical EUS Activation to Relax Sensitized Micturition Response Life external urethral sphincter striated muscle optogenetics micturition continence |
| title | Optical EUS Activation to Relax Sensitized Micturition Response |
| title_full | Optical EUS Activation to Relax Sensitized Micturition Response |
| title_fullStr | Optical EUS Activation to Relax Sensitized Micturition Response |
| title_full_unstemmed | Optical EUS Activation to Relax Sensitized Micturition Response |
| title_short | Optical EUS Activation to Relax Sensitized Micturition Response |
| title_sort | optical eus activation to relax sensitized micturition response |
| topic | external urethral sphincter striated muscle optogenetics micturition continence |
| url | https://www.mdpi.com/2075-1729/13/10/1961 |
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