The Alleviation of Gut Microbiota-Induced Depression and Colitis in Mice by Anti-Inflammatory Probiotics NK151, NK173, and NK175
Gut microbiota dysbiosis is strongly associated with psychiatric disorders and inflammatory bowel disease (IBD). Herein, we examined whether the fecal microbiota of IBD patients with depression (IBDD) and their gut microbiota culture (iGm) could cause depression and colitis in mice and anti-inflamma...
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MDPI AG
2022-05-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/14/10/2080 |
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| author | Jong-Wook Yoo Yoon-Jung Shin Xiaoyang Ma Young-Hoo Son Hyo-Min Jang Chang Kyun Lee Dong-Hyun Kim |
| author_facet | Jong-Wook Yoo Yoon-Jung Shin Xiaoyang Ma Young-Hoo Son Hyo-Min Jang Chang Kyun Lee Dong-Hyun Kim |
| author_sort | Jong-Wook Yoo |
| collection | DOAJ |
| description | Gut microbiota dysbiosis is strongly associated with psychiatric disorders and inflammatory bowel disease (IBD). Herein, we examined whether the fecal microbiota of IBD patients with depression (IBDD) and their gut microbiota culture (iGm) could cause depression and colitis in mice and anti-inflammatory probiotics could mitigate depression in iGm-transplanted or immobilization stress (IS)-exposed mice. Fecal microbiota transplantation (FMT) from IBDD patients, which exhibited <i>Enterobacteriaceae</i>-rich gut microbiota, and its gut microbiota culture (iGm) increased depression-like behaviors in mice. Their treatments heightened the blood lipopolysaccharide (LPS) level and colonic IL-1β and IL-6 expression. However, FMT from healthy volunteers or sulfasalazine treatment alleviated cGm-induced depressive-like behaviors and hippocampal and colonic inflammation in mice. Moreover, oral administration of <i>Lactobacillus plantarum</i> NK151, <i>Bifidobacterium longum</i> NK173, and <i>Bifidobacterium bifidum</i> NK175, which inhibited LPS-induced IL-6 expression in macrophages, alleviated cGm-induced depression-like behaviors, hippocampal NF-κB<sup>+</sup>Iba1<sup>+</sup> cell numbers and IL-1β and IL-6 expression, blood LPS, IL-6, and creatinine levels, and colonic NF-κB<sup>+</sup>CD11c<sup>+</sup> number and IL-1β and IL-6 expression in mice. Treatment with NK151, NK173, or NK175 mitigated immobilization stress (IS)-induced depressive-like behaviors, neuroinflammation, and gut inflammation in mice. NK151, NK173, or NK175 also decreased IS-induced blood LPS, IL-6, and creatinine levels. The transplantation of <i>Enterobacteriaceae</i>-rich gut microbiota can cause depression and colitis, as IS exposure, and anti-inflammatory NK151, NK173, and NK175, may alleviate stress-induced fatigue, depression, and colitis by regulating the expression of proinflammatory and anti-inflammatory cytokines through the suppression of gut bacterial LPS. |
| first_indexed | 2024-03-10T03:14:26Z |
| format | Article |
| id | doaj.art-70fbbdadc2b249da937dd2d2a192e8ca |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-10T03:14:26Z |
| publishDate | 2022-05-01 |
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| spelling | doaj.art-70fbbdadc2b249da937dd2d2a192e8ca2023-11-23T12:29:43ZengMDPI AGNutrients2072-66432022-05-011410208010.3390/nu14102080The Alleviation of Gut Microbiota-Induced Depression and Colitis in Mice by Anti-Inflammatory Probiotics NK151, NK173, and NK175Jong-Wook Yoo0Yoon-Jung Shin1Xiaoyang Ma2Young-Hoo Son3Hyo-Min Jang4Chang Kyun Lee5Dong-Hyun Kim6Neurobiota Research Center, College of Pharmacy, Kyung Hee University, 26, Kyungheedae-ro, Dongdaemun-gu, Seoul 02447, KoreaNeurobiota Research Center, College of Pharmacy, Kyung Hee University, 26, Kyungheedae-ro, Dongdaemun-gu, Seoul 02447, KoreaNeurobiota Research Center, College of Pharmacy, Kyung Hee University, 26, Kyungheedae-ro, Dongdaemun-gu, Seoul 02447, KoreaNeurobiota Research Center, College of Pharmacy, Kyung Hee University, 26, Kyungheedae-ro, Dongdaemun-gu, Seoul 02447, KoreaNeurobiota Research Center, College of Pharmacy, Kyung Hee University, 26, Kyungheedae-ro, Dongdaemun-gu, Seoul 02447, KoreaDepartment of Internal Medicine, School of Medicine, Kyung Hee University, Seoul 02447, KoreaNeurobiota Research Center, College of Pharmacy, Kyung Hee University, 26, Kyungheedae-ro, Dongdaemun-gu, Seoul 02447, KoreaGut microbiota dysbiosis is strongly associated with psychiatric disorders and inflammatory bowel disease (IBD). Herein, we examined whether the fecal microbiota of IBD patients with depression (IBDD) and their gut microbiota culture (iGm) could cause depression and colitis in mice and anti-inflammatory probiotics could mitigate depression in iGm-transplanted or immobilization stress (IS)-exposed mice. Fecal microbiota transplantation (FMT) from IBDD patients, which exhibited <i>Enterobacteriaceae</i>-rich gut microbiota, and its gut microbiota culture (iGm) increased depression-like behaviors in mice. Their treatments heightened the blood lipopolysaccharide (LPS) level and colonic IL-1β and IL-6 expression. However, FMT from healthy volunteers or sulfasalazine treatment alleviated cGm-induced depressive-like behaviors and hippocampal and colonic inflammation in mice. Moreover, oral administration of <i>Lactobacillus plantarum</i> NK151, <i>Bifidobacterium longum</i> NK173, and <i>Bifidobacterium bifidum</i> NK175, which inhibited LPS-induced IL-6 expression in macrophages, alleviated cGm-induced depression-like behaviors, hippocampal NF-κB<sup>+</sup>Iba1<sup>+</sup> cell numbers and IL-1β and IL-6 expression, blood LPS, IL-6, and creatinine levels, and colonic NF-κB<sup>+</sup>CD11c<sup>+</sup> number and IL-1β and IL-6 expression in mice. Treatment with NK151, NK173, or NK175 mitigated immobilization stress (IS)-induced depressive-like behaviors, neuroinflammation, and gut inflammation in mice. NK151, NK173, or NK175 also decreased IS-induced blood LPS, IL-6, and creatinine levels. The transplantation of <i>Enterobacteriaceae</i>-rich gut microbiota can cause depression and colitis, as IS exposure, and anti-inflammatory NK151, NK173, and NK175, may alleviate stress-induced fatigue, depression, and colitis by regulating the expression of proinflammatory and anti-inflammatory cytokines through the suppression of gut bacterial LPS.https://www.mdpi.com/2072-6643/14/10/2080gut dysbiosisgut microbiome culturedepressioncolitis<i>Lactobacillus plantarum</i><i>Bifidobacterium longum</i> |
| spellingShingle | Jong-Wook Yoo Yoon-Jung Shin Xiaoyang Ma Young-Hoo Son Hyo-Min Jang Chang Kyun Lee Dong-Hyun Kim The Alleviation of Gut Microbiota-Induced Depression and Colitis in Mice by Anti-Inflammatory Probiotics NK151, NK173, and NK175 Nutrients gut dysbiosis gut microbiome culture depression colitis <i>Lactobacillus plantarum</i> <i>Bifidobacterium longum</i> |
| title | The Alleviation of Gut Microbiota-Induced Depression and Colitis in Mice by Anti-Inflammatory Probiotics NK151, NK173, and NK175 |
| title_full | The Alleviation of Gut Microbiota-Induced Depression and Colitis in Mice by Anti-Inflammatory Probiotics NK151, NK173, and NK175 |
| title_fullStr | The Alleviation of Gut Microbiota-Induced Depression and Colitis in Mice by Anti-Inflammatory Probiotics NK151, NK173, and NK175 |
| title_full_unstemmed | The Alleviation of Gut Microbiota-Induced Depression and Colitis in Mice by Anti-Inflammatory Probiotics NK151, NK173, and NK175 |
| title_short | The Alleviation of Gut Microbiota-Induced Depression and Colitis in Mice by Anti-Inflammatory Probiotics NK151, NK173, and NK175 |
| title_sort | alleviation of gut microbiota induced depression and colitis in mice by anti inflammatory probiotics nk151 nk173 and nk175 |
| topic | gut dysbiosis gut microbiome culture depression colitis <i>Lactobacillus plantarum</i> <i>Bifidobacterium longum</i> |
| url | https://www.mdpi.com/2072-6643/14/10/2080 |
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