Effect of TET inhibitor on bovine parthenogenetic embryo development.
DNA demethylation catalysed by the ten-eleven translocation (TET) protein is an important step during extensive global epigenetic reprogramming in mammals. However, whether TET proteins play a key role in DNA demethylation during the development of bovine pre-implanted embryos is still unclear. In t...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2017-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5739418?pdf=render |
| _version_ | 1819062668200247296 |
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| author | Jian Zhang Sheng Zhang Yutian Wang Hui Cheng Linlin Hao Yanhui Zhai Zhiren Zhang Xinglan An Xiaoling Ma Xueming Zhang Ziyi Li Bo Tang |
| author_facet | Jian Zhang Sheng Zhang Yutian Wang Hui Cheng Linlin Hao Yanhui Zhai Zhiren Zhang Xinglan An Xiaoling Ma Xueming Zhang Ziyi Li Bo Tang |
| author_sort | Jian Zhang |
| collection | DOAJ |
| description | DNA demethylation catalysed by the ten-eleven translocation (TET) protein is an important step during extensive global epigenetic reprogramming in mammals. However, whether TET proteins play a key role in DNA demethylation during the development of bovine pre-implanted embryos is still unclear. In this study, we utilized dimethyloxallyl glycine (DMOG), a small-molecule inhibitor of the TET protein, to impede the enzymatic activity of TET and explore subsequent effects on bovine parthenogenetic embryo development. We first detected the expression of the TET family, consisting of TET1, TET2 and TET3, in bovine MII stage oocytes and found that TET3 is more highly expressed than TET1 and TET2. Treatment with 1 mM DMOG increased 5mC levels (30.4% vs 79.8% at the 8-cell stage for satellite I, 25.3% vs 40.6% at the 8-cell stage for α-satellite, 20.5% vs 73.5% at the blastocyst stage for satellite I and 16.6% vs 30.0% at the blastocyst stage for α-satellite) at every bovine parthenogenetic embryo developmental stage. At the same time, DNA methylation level of satellite DNA and pluripotency gene promoters increased significantly. Real-time PCR analysis results indicated that the transcription levels of NANOG and OCT-4 decreased in the DMOG-treated group. Furthermore, TET inhibition negatively affected blastocyst formation, resulting in a decline in the blastocyst rate (17.1 ± 1.3% vs 24.1 ± 0.6%); however, the percentage of apoptotic cells was significantly increased according to the results of a TUNEL assay. Additionally, expression levels of the apoptosis-related gene BAX were up-regulated, while the expression of BCL-2 was down-regulated. In conclusion, these results support that TET plays important roles in bovine parthenogenetic embryo development by influencing DNA methylation reprogramming, gene expression and apoptosis. |
| first_indexed | 2024-12-21T15:02:26Z |
| format | Article |
| id | doaj.art-70ff51d28820414898bde996ce11dfa2 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-21T15:02:26Z |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
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| spelling | doaj.art-70ff51d28820414898bde996ce11dfa22022-12-21T18:59:33ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-011212e018954210.1371/journal.pone.0189542Effect of TET inhibitor on bovine parthenogenetic embryo development.Jian ZhangSheng ZhangYutian WangHui ChengLinlin HaoYanhui ZhaiZhiren ZhangXinglan AnXiaoling MaXueming ZhangZiyi LiBo TangDNA demethylation catalysed by the ten-eleven translocation (TET) protein is an important step during extensive global epigenetic reprogramming in mammals. However, whether TET proteins play a key role in DNA demethylation during the development of bovine pre-implanted embryos is still unclear. In this study, we utilized dimethyloxallyl glycine (DMOG), a small-molecule inhibitor of the TET protein, to impede the enzymatic activity of TET and explore subsequent effects on bovine parthenogenetic embryo development. We first detected the expression of the TET family, consisting of TET1, TET2 and TET3, in bovine MII stage oocytes and found that TET3 is more highly expressed than TET1 and TET2. Treatment with 1 mM DMOG increased 5mC levels (30.4% vs 79.8% at the 8-cell stage for satellite I, 25.3% vs 40.6% at the 8-cell stage for α-satellite, 20.5% vs 73.5% at the blastocyst stage for satellite I and 16.6% vs 30.0% at the blastocyst stage for α-satellite) at every bovine parthenogenetic embryo developmental stage. At the same time, DNA methylation level of satellite DNA and pluripotency gene promoters increased significantly. Real-time PCR analysis results indicated that the transcription levels of NANOG and OCT-4 decreased in the DMOG-treated group. Furthermore, TET inhibition negatively affected blastocyst formation, resulting in a decline in the blastocyst rate (17.1 ± 1.3% vs 24.1 ± 0.6%); however, the percentage of apoptotic cells was significantly increased according to the results of a TUNEL assay. Additionally, expression levels of the apoptosis-related gene BAX were up-regulated, while the expression of BCL-2 was down-regulated. In conclusion, these results support that TET plays important roles in bovine parthenogenetic embryo development by influencing DNA methylation reprogramming, gene expression and apoptosis.http://europepmc.org/articles/PMC5739418?pdf=render |
| spellingShingle | Jian Zhang Sheng Zhang Yutian Wang Hui Cheng Linlin Hao Yanhui Zhai Zhiren Zhang Xinglan An Xiaoling Ma Xueming Zhang Ziyi Li Bo Tang Effect of TET inhibitor on bovine parthenogenetic embryo development. PLoS ONE |
| title | Effect of TET inhibitor on bovine parthenogenetic embryo development. |
| title_full | Effect of TET inhibitor on bovine parthenogenetic embryo development. |
| title_fullStr | Effect of TET inhibitor on bovine parthenogenetic embryo development. |
| title_full_unstemmed | Effect of TET inhibitor on bovine parthenogenetic embryo development. |
| title_short | Effect of TET inhibitor on bovine parthenogenetic embryo development. |
| title_sort | effect of tet inhibitor on bovine parthenogenetic embryo development |
| url | http://europepmc.org/articles/PMC5739418?pdf=render |
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