Nanopore Technology for the Application of Protein Detection
Protein is an important component of all the cells and tissues of the human body and is the material basis of life. Its content, sequence, and spatial structure have a great impact on proteomics and human biology. It can reflect the important information of normal or pathophysiological processes and...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-07-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/11/8/1942 |
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| author | Xiaoqing Zeng Yang Xiang Qianshan Liu Liang Wang Qianyun Ma Wenhao Ma Delin Zeng Yajie Yin Deqiang Wang |
| author_facet | Xiaoqing Zeng Yang Xiang Qianshan Liu Liang Wang Qianyun Ma Wenhao Ma Delin Zeng Yajie Yin Deqiang Wang |
| author_sort | Xiaoqing Zeng |
| collection | DOAJ |
| description | Protein is an important component of all the cells and tissues of the human body and is the material basis of life. Its content, sequence, and spatial structure have a great impact on proteomics and human biology. It can reflect the important information of normal or pathophysiological processes and promote the development of new diagnoses and treatment methods. However, the current techniques of proteomics for protein analysis are limited by chemical modifications, large sample sizes, or cumbersome operations. Solving this problem requires overcoming huge challenges. Nanopore single molecule detection technology overcomes this shortcoming. As a new sensing technology, it has the advantages of no labeling, high sensitivity, fast detection speed, real-time monitoring, and simple operation. It is widely used in gene sequencing, detection of peptides and proteins, markers and microorganisms, and other biomolecules and metal ions. Therefore, based on the advantages of novel nanopore single-molecule detection technology, its application to protein sequence detection and structure recognition has also been proposed and developed. In this paper, the application of nanopore single-molecule detection technology in protein detection in recent years is reviewed, and its development prospect is investigated. |
| first_indexed | 2024-03-10T08:32:17Z |
| format | Article |
| id | doaj.art-7107e21d797743b481bb6a47e7b2bac6 |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-10T08:32:17Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-7107e21d797743b481bb6a47e7b2bac62023-11-22T08:58:05ZengMDPI AGNanomaterials2079-49912021-07-01118194210.3390/nano11081942Nanopore Technology for the Application of Protein DetectionXiaoqing Zeng0Yang Xiang1Qianshan Liu2Liang Wang3Qianyun Ma4Wenhao Ma5Delin Zeng6Yajie Yin7Deqiang Wang8Chongqing University, 174 Shazheng Street, Shapingba District, Chongqing 400044, ChinaChongqing University, 174 Shazheng Street, Shapingba District, Chongqing 400044, ChinaChongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing 400714, ChinaChongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing 400714, ChinaChongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing 400714, ChinaChongqing University, 174 Shazheng Street, Shapingba District, Chongqing 400044, ChinaChongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing 400714, ChinaChongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing 400714, ChinaChongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing 400714, ChinaProtein is an important component of all the cells and tissues of the human body and is the material basis of life. Its content, sequence, and spatial structure have a great impact on proteomics and human biology. It can reflect the important information of normal or pathophysiological processes and promote the development of new diagnoses and treatment methods. However, the current techniques of proteomics for protein analysis are limited by chemical modifications, large sample sizes, or cumbersome operations. Solving this problem requires overcoming huge challenges. Nanopore single molecule detection technology overcomes this shortcoming. As a new sensing technology, it has the advantages of no labeling, high sensitivity, fast detection speed, real-time monitoring, and simple operation. It is widely used in gene sequencing, detection of peptides and proteins, markers and microorganisms, and other biomolecules and metal ions. Therefore, based on the advantages of novel nanopore single-molecule detection technology, its application to protein sequence detection and structure recognition has also been proposed and developed. In this paper, the application of nanopore single-molecule detection technology in protein detection in recent years is reviewed, and its development prospect is investigated.https://www.mdpi.com/2079-4991/11/8/1942nanoporesingle-molecule detectionprotein detectionsolid-state nanopore |
| spellingShingle | Xiaoqing Zeng Yang Xiang Qianshan Liu Liang Wang Qianyun Ma Wenhao Ma Delin Zeng Yajie Yin Deqiang Wang Nanopore Technology for the Application of Protein Detection Nanomaterials nanopore single-molecule detection protein detection solid-state nanopore |
| title | Nanopore Technology for the Application of Protein Detection |
| title_full | Nanopore Technology for the Application of Protein Detection |
| title_fullStr | Nanopore Technology for the Application of Protein Detection |
| title_full_unstemmed | Nanopore Technology for the Application of Protein Detection |
| title_short | Nanopore Technology for the Application of Protein Detection |
| title_sort | nanopore technology for the application of protein detection |
| topic | nanopore single-molecule detection protein detection solid-state nanopore |
| url | https://www.mdpi.com/2079-4991/11/8/1942 |
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