<it>In vitro </it>antimicrobial effects of aztreonam, colistin, and the 3-drug combination of aztreonam, ceftazidime and amikacin on metallo-<it>β</it>-lactamase-producing <it>Pseudomonas aeruginosa</it>
<p>Abstract</p> <p>Background</p> <p>There are limited choice of antimicrobial agents to treat infection with metallo-<it>β</it>-lactamase-producing <it>Pseudomonas aeruginosa</it>. We evaluate the antimicrobial effects of aztreonam alone, colist...
Main Authors: | , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
BMC
2009-08-01
|
Series: | BMC Infectious Diseases |
Online Access: | http://www.biomedcentral.com/1471-2334/9/123 |
_version_ | 1811271229844226048 |
---|---|
author | Oie Shigeharu Fukui Yumi Yamamoto Masaya Masuda Yuki Kamiya Akira |
author_facet | Oie Shigeharu Fukui Yumi Yamamoto Masaya Masuda Yuki Kamiya Akira |
author_sort | Oie Shigeharu |
collection | DOAJ |
description | <p>Abstract</p> <p>Background</p> <p>There are limited choice of antimicrobial agents to treat infection with metallo-<it>β</it>-lactamase-producing <it>Pseudomonas aeruginosa</it>. We evaluate the antimicrobial effects of aztreonam alone, colistin alone and the 3-drug combination of aztreonam, ceftazidime and amikacin on 23 strains of metallo-<it>β</it>-lactamase-producing <it>P. aeruginosa </it>by time-killing tests.</p> <p>Methods</p> <p>Strains used were from different hospitals in Japan and had different pulse-field gel electrophoresis patterns by restriction with <it>Spe</it>I. The minimum inhibitory concentrations of 11 antimicrobial agents (piperacillin, piperacillin/tazobactam, imipenem, meropenem, aztreonam, ceftazidime, amikacin, tobramycin, arbekacin, ciprofloxacin and colistin) were determined using the agar dilution test. The effects of aztreonam, colistin and the combination of aztreonam, ceftazidime and amikacin were determined by time-killing studies.</p> <p>Results</p> <p>Bacteriostatic effects after 6 hours of drug exposure were observed in 12 strains (52.2%) of 23 strains of metallo-<it>β</it>-lactamase-producing <it>P. aeruginosa </it>with 48 mg/l aztreonam, in 19 strains (82.6%) with the 3-drug combination of 16 mg/l aztreonam, 16 mg/l ceftazidime, and 4 mg/l amikacin, and in 23 strains (100%) with 2 mg/l colistin. Bactericidal effects after 6 h drug exposure were observed in 1 strain (4.3%) with 48 mg/l aztreonam, in 8 strains (30.4%) with the 3-drug combination and in all 23 strains (100%) with 2 mg/l colistin.</p> <p>Conclusion</p> <p>Evaluation of <it>in vitro </it>antimicrobial effects on metallo-<it>β</it>-lactamase-producing <it>P. aeruginosa </it>revealed relatively good effects of the 3-drug combination of aztreonam, ceftazidime and amikacin and marked effects of colistin.</p> |
first_indexed | 2024-04-12T22:17:28Z |
format | Article |
id | doaj.art-7109de77ea5a4129a89a1a96126aa959 |
institution | Directory Open Access Journal |
issn | 1471-2334 |
language | English |
last_indexed | 2024-04-12T22:17:28Z |
publishDate | 2009-08-01 |
publisher | BMC |
record_format | Article |
series | BMC Infectious Diseases |
spelling | doaj.art-7109de77ea5a4129a89a1a96126aa9592022-12-22T03:14:30ZengBMCBMC Infectious Diseases1471-23342009-08-019112310.1186/1471-2334-9-123<it>In vitro </it>antimicrobial effects of aztreonam, colistin, and the 3-drug combination of aztreonam, ceftazidime and amikacin on metallo-<it>β</it>-lactamase-producing <it>Pseudomonas aeruginosa</it>Oie ShigeharuFukui YumiYamamoto MasayaMasuda YukiKamiya Akira<p>Abstract</p> <p>Background</p> <p>There are limited choice of antimicrobial agents to treat infection with metallo-<it>β</it>-lactamase-producing <it>Pseudomonas aeruginosa</it>. We evaluate the antimicrobial effects of aztreonam alone, colistin alone and the 3-drug combination of aztreonam, ceftazidime and amikacin on 23 strains of metallo-<it>β</it>-lactamase-producing <it>P. aeruginosa </it>by time-killing tests.</p> <p>Methods</p> <p>Strains used were from different hospitals in Japan and had different pulse-field gel electrophoresis patterns by restriction with <it>Spe</it>I. The minimum inhibitory concentrations of 11 antimicrobial agents (piperacillin, piperacillin/tazobactam, imipenem, meropenem, aztreonam, ceftazidime, amikacin, tobramycin, arbekacin, ciprofloxacin and colistin) were determined using the agar dilution test. The effects of aztreonam, colistin and the combination of aztreonam, ceftazidime and amikacin were determined by time-killing studies.</p> <p>Results</p> <p>Bacteriostatic effects after 6 hours of drug exposure were observed in 12 strains (52.2%) of 23 strains of metallo-<it>β</it>-lactamase-producing <it>P. aeruginosa </it>with 48 mg/l aztreonam, in 19 strains (82.6%) with the 3-drug combination of 16 mg/l aztreonam, 16 mg/l ceftazidime, and 4 mg/l amikacin, and in 23 strains (100%) with 2 mg/l colistin. Bactericidal effects after 6 h drug exposure were observed in 1 strain (4.3%) with 48 mg/l aztreonam, in 8 strains (30.4%) with the 3-drug combination and in all 23 strains (100%) with 2 mg/l colistin.</p> <p>Conclusion</p> <p>Evaluation of <it>in vitro </it>antimicrobial effects on metallo-<it>β</it>-lactamase-producing <it>P. aeruginosa </it>revealed relatively good effects of the 3-drug combination of aztreonam, ceftazidime and amikacin and marked effects of colistin.</p>http://www.biomedcentral.com/1471-2334/9/123 |
spellingShingle | Oie Shigeharu Fukui Yumi Yamamoto Masaya Masuda Yuki Kamiya Akira <it>In vitro </it>antimicrobial effects of aztreonam, colistin, and the 3-drug combination of aztreonam, ceftazidime and amikacin on metallo-<it>β</it>-lactamase-producing <it>Pseudomonas aeruginosa</it> BMC Infectious Diseases |
title | <it>In vitro </it>antimicrobial effects of aztreonam, colistin, and the 3-drug combination of aztreonam, ceftazidime and amikacin on metallo-<it>β</it>-lactamase-producing <it>Pseudomonas aeruginosa</it> |
title_full | <it>In vitro </it>antimicrobial effects of aztreonam, colistin, and the 3-drug combination of aztreonam, ceftazidime and amikacin on metallo-<it>β</it>-lactamase-producing <it>Pseudomonas aeruginosa</it> |
title_fullStr | <it>In vitro </it>antimicrobial effects of aztreonam, colistin, and the 3-drug combination of aztreonam, ceftazidime and amikacin on metallo-<it>β</it>-lactamase-producing <it>Pseudomonas aeruginosa</it> |
title_full_unstemmed | <it>In vitro </it>antimicrobial effects of aztreonam, colistin, and the 3-drug combination of aztreonam, ceftazidime and amikacin on metallo-<it>β</it>-lactamase-producing <it>Pseudomonas aeruginosa</it> |
title_short | <it>In vitro </it>antimicrobial effects of aztreonam, colistin, and the 3-drug combination of aztreonam, ceftazidime and amikacin on metallo-<it>β</it>-lactamase-producing <it>Pseudomonas aeruginosa</it> |
title_sort | it in vitro it antimicrobial effects of aztreonam colistin and the 3 drug combination of aztreonam ceftazidime and amikacin on metallo it β it lactamase producing it pseudomonas aeruginosa it |
url | http://www.biomedcentral.com/1471-2334/9/123 |
work_keys_str_mv | AT oieshigeharu itinvitroitantimicrobialeffectsofaztreonamcolistinandthe3drugcombinationofaztreonamceftazidimeandamikacinonmetalloitbitlactamaseproducingitpseudomonasaeruginosait AT fukuiyumi itinvitroitantimicrobialeffectsofaztreonamcolistinandthe3drugcombinationofaztreonamceftazidimeandamikacinonmetalloitbitlactamaseproducingitpseudomonasaeruginosait AT yamamotomasaya itinvitroitantimicrobialeffectsofaztreonamcolistinandthe3drugcombinationofaztreonamceftazidimeandamikacinonmetalloitbitlactamaseproducingitpseudomonasaeruginosait AT masudayuki itinvitroitantimicrobialeffectsofaztreonamcolistinandthe3drugcombinationofaztreonamceftazidimeandamikacinonmetalloitbitlactamaseproducingitpseudomonasaeruginosait AT kamiyaakira itinvitroitantimicrobialeffectsofaztreonamcolistinandthe3drugcombinationofaztreonamceftazidimeandamikacinonmetalloitbitlactamaseproducingitpseudomonasaeruginosait |