Systematic analysis of the sugar accumulation mechanism in sucrose- and hexose- accumulating cherry tomato fruits
Abstract Background Sugar content is an important indicator of fruit quality. Except for a few wild tomato species that accumulate sucrose in the fruits, most cultivated tomato species accumulate hexose. Although several studies have focused on wild sucrose-accumulating tomato, the sucrose accumulat...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-06-01
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| Series: | BMC Plant Biology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12870-022-03685-8 |
| _version_ | 1818249898137485312 |
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| author | Lulu Sun Jianli Wang Liqiang Lian Jian Song Xueni Du Wenke Liu Wenchao Zhao Liu Yang Changbao Li Yong Qin Rui Yang |
| author_facet | Lulu Sun Jianli Wang Liqiang Lian Jian Song Xueni Du Wenke Liu Wenchao Zhao Liu Yang Changbao Li Yong Qin Rui Yang |
| author_sort | Lulu Sun |
| collection | DOAJ |
| description | Abstract Background Sugar content is an important indicator of fruit quality. Except for a few wild tomato species that accumulate sucrose in the fruits, most cultivated tomato species accumulate hexose. Although several studies have focused on wild sucrose-accumulating tomato, the sucrose accumulation mechanism is still unclear. Results Here, two homozygous inbred cherry tomato lines (‘TB0023’ and ‘TB0278’, which accumulated sucrose and hexose, respectively) were selected to analyze the sugar accumulation mechanism. Carbohydrate analysis, cytological observation, gene expression and enzyme activity analysis and proteomics methods were used in this study. The results indicated that glucose and fructose were absolutely dominant in the soluble sugar content of hexose-accumulating cherry tomato fruit, while sucrose and a certain proportion of hexose were the main forms of soluble sugar in sucrose-accumulating cherry tomato fruit. The phloem unloading pathway of the hexose-accumulating cherry tomato fruit switched from symplastic to apoplastic during fruit development, and the sucrose-accumulating cherry tomato probably had a mixed unloading pathway involving the symplastic and apoplastic. High activity of acid invertase (AI), sucrose phosphate synthase (SPS), sucrose synthase (SS) and sugar transporters LeSUT1, SlSWEET2a and SlSWEET12c were important factors for hexose accumulation in the hexose-accumulating cherry tomato fruit, while LeSUT2, SPS, SS, SlSWEET1b, SlSWEET5b, SlSWEET11b, SlSWEET7a, SlSWEET14 were responsible for solute sugar accumulation in the sucrose-accumulating cherry tomato. Conclusions This study provides detailed evidence for elucidation of the tomato sugar accumulation mechanism from the perspective of cell structure, physiology and molecular biology, providing a theoretical basis for the improvement of tomato quality and aiding the utilization of tomato genetic resources. |
| first_indexed | 2024-12-12T15:43:48Z |
| format | Article |
| id | doaj.art-710acf135188444fb53f108d21430589 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-12-12T15:43:48Z |
| publishDate | 2022-06-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-710acf135188444fb53f108d214305892022-12-22T00:19:50ZengBMCBMC Plant Biology1471-22292022-06-0122111610.1186/s12870-022-03685-8Systematic analysis of the sugar accumulation mechanism in sucrose- and hexose- accumulating cherry tomato fruitsLulu Sun0Jianli Wang1Liqiang Lian2Jian Song3Xueni Du4Wenke Liu5Wenchao Zhao6Liu Yang7Changbao Li8Yong Qin9Rui Yang10Beijing Key Laboratory for Agricultural Application and New Technique, Beijing University of AgricultureBeijing Key Laboratory for Agricultural Application and New Technique, Beijing University of AgricultureCollege of Plant Science and Technology, Beijing University of AgricultureCollege of Plant Science and Technology, Beijing University of AgricultureCollege of Plant Science and Technology, Beijing University of AgricultureCollege of Plant Science and Technology, Beijing University of AgricultureBeijing Key Laboratory for Agricultural Application and New Technique, Beijing University of AgricultureBeijing Key Laboratory for Agricultural Application and New Technique, Beijing University of AgricultureBeijing Vegetable Research Centre, Beijing Academy of Agriculture and Forestry SciencesDepartment of Forestry and Horticulture, Xinjiang Agricultural UniversityBeijing Key Laboratory for Agricultural Application and New Technique, Beijing University of AgricultureAbstract Background Sugar content is an important indicator of fruit quality. Except for a few wild tomato species that accumulate sucrose in the fruits, most cultivated tomato species accumulate hexose. Although several studies have focused on wild sucrose-accumulating tomato, the sucrose accumulation mechanism is still unclear. Results Here, two homozygous inbred cherry tomato lines (‘TB0023’ and ‘TB0278’, which accumulated sucrose and hexose, respectively) were selected to analyze the sugar accumulation mechanism. Carbohydrate analysis, cytological observation, gene expression and enzyme activity analysis and proteomics methods were used in this study. The results indicated that glucose and fructose were absolutely dominant in the soluble sugar content of hexose-accumulating cherry tomato fruit, while sucrose and a certain proportion of hexose were the main forms of soluble sugar in sucrose-accumulating cherry tomato fruit. The phloem unloading pathway of the hexose-accumulating cherry tomato fruit switched from symplastic to apoplastic during fruit development, and the sucrose-accumulating cherry tomato probably had a mixed unloading pathway involving the symplastic and apoplastic. High activity of acid invertase (AI), sucrose phosphate synthase (SPS), sucrose synthase (SS) and sugar transporters LeSUT1, SlSWEET2a and SlSWEET12c were important factors for hexose accumulation in the hexose-accumulating cherry tomato fruit, while LeSUT2, SPS, SS, SlSWEET1b, SlSWEET5b, SlSWEET11b, SlSWEET7a, SlSWEET14 were responsible for solute sugar accumulation in the sucrose-accumulating cherry tomato. Conclusions This study provides detailed evidence for elucidation of the tomato sugar accumulation mechanism from the perspective of cell structure, physiology and molecular biology, providing a theoretical basis for the improvement of tomato quality and aiding the utilization of tomato genetic resources.https://doi.org/10.1186/s12870-022-03685-8SucroseHexoseAccumulationCherry tomatoPhloem unloading |
| spellingShingle | Lulu Sun Jianli Wang Liqiang Lian Jian Song Xueni Du Wenke Liu Wenchao Zhao Liu Yang Changbao Li Yong Qin Rui Yang Systematic analysis of the sugar accumulation mechanism in sucrose- and hexose- accumulating cherry tomato fruits BMC Plant Biology Sucrose Hexose Accumulation Cherry tomato Phloem unloading |
| title | Systematic analysis of the sugar accumulation mechanism in sucrose- and hexose- accumulating cherry tomato fruits |
| title_full | Systematic analysis of the sugar accumulation mechanism in sucrose- and hexose- accumulating cherry tomato fruits |
| title_fullStr | Systematic analysis of the sugar accumulation mechanism in sucrose- and hexose- accumulating cherry tomato fruits |
| title_full_unstemmed | Systematic analysis of the sugar accumulation mechanism in sucrose- and hexose- accumulating cherry tomato fruits |
| title_short | Systematic analysis of the sugar accumulation mechanism in sucrose- and hexose- accumulating cherry tomato fruits |
| title_sort | systematic analysis of the sugar accumulation mechanism in sucrose and hexose accumulating cherry tomato fruits |
| topic | Sucrose Hexose Accumulation Cherry tomato Phloem unloading |
| url | https://doi.org/10.1186/s12870-022-03685-8 |
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