Transcriptomic Signature of Horseshoe Crab <i>Carcinoscorpius rotundicauda</i> Hemocytes’ Response to Lipopolysaccharides
<i>Carcinoscorpius rotundicauda</i> (<i>C. rotundicauda</i>) is one of the four species of horseshoe crabs (HSCs). The HSC hemocytes store defense molecules that are released upon encountering invading pathogens. The HSCs rely on this innate immunity to continue its existence...
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MDPI AG
2022-11-01
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| Series: | Current Issues in Molecular Biology |
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| Online Access: | https://www.mdpi.com/1467-3045/44/12/399 |
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| author | Maria E. Sarmiento Kai Ling Chin Nyok-Sean Lau Noraznawati Ismail Mohd Nor Norazmi Armando Acosta Nik Soriani Yaacob |
| author_facet | Maria E. Sarmiento Kai Ling Chin Nyok-Sean Lau Noraznawati Ismail Mohd Nor Norazmi Armando Acosta Nik Soriani Yaacob |
| author_sort | Maria E. Sarmiento |
| collection | DOAJ |
| description | <i>Carcinoscorpius rotundicauda</i> (<i>C. rotundicauda</i>) is one of the four species of horseshoe crabs (HSCs). The HSC hemocytes store defense molecules that are released upon encountering invading pathogens. The HSCs rely on this innate immunity to continue its existence as a living fossil for more than 480 million years. To gain insight into the innate mechanisms involved, transcriptomic analysis was performed on isolated <i>C. rotundicauda</i> hemocytes challenged with lipopolysaccharides (LPS), the main components of the outer cell membrane of gram-negative bacteria. RNA-sequencing with Illumina HiSeq platform resulted in 232,628,086 and 245,448,176 raw reads corresponding to 190,326,253 and 201,180,020 high-quality mappable reads from control and LPS-stimulated hemocytes, respectively. Following LPS-stimulation, 79 genes were significantly upregulated and 265 genes were downregulated. The differentially expressed genes (DEGs) were related to multiple immune functional categories and pathways such as those of the cytoskeleton, Toll and Imd, apoptosis, MAP kinase (MAPK), inositol phosphate metabolism, phagosome, leucocyte endothelial migration, and gram-negative bacterial infection, among others. This study provides important information about the mechanisms of response to LPS, which is relevant for the understanding the HSCs’ immune response. |
| first_indexed | 2024-03-09T17:11:29Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1467-3037 1467-3045 |
| language | English |
| last_indexed | 2024-03-09T17:11:29Z |
| publishDate | 2022-11-01 |
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| record_format | Article |
| series | Current Issues in Molecular Biology |
| spelling | doaj.art-710c80f6dc5e41cc91b3931b309724712023-11-24T14:03:23ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452022-11-0144125866587810.3390/cimb44120399Transcriptomic Signature of Horseshoe Crab <i>Carcinoscorpius rotundicauda</i> Hemocytes’ Response to LipopolysaccharidesMaria E. Sarmiento0Kai Ling Chin1Nyok-Sean Lau2Noraznawati Ismail3Mohd Nor Norazmi4Armando Acosta5Nik Soriani Yaacob6School of Health Sciences, Universiti Sains Malaysia, Health Campus, Kubang Kerian 16150, MalaysiaFaculty of Medicine and Health Sciences, Universiti Malaysia Sabah, Kota Kinabalu 88400, MalaysiaCentre for Chemical Biology, Universiti Sains Malaysia, Bayan Lepas 11900, MalaysiaInstitute of Marine Biotechnology, Universiti Malaysia Terengganu, Kuala Nerus 21030, MalaysiaSchool of Health Sciences, Universiti Sains Malaysia, Health Campus, Kubang Kerian 16150, MalaysiaSchool of Health Sciences, Universiti Sains Malaysia, Health Campus, Kubang Kerian 16150, MalaysiaDepartment of Chemical Pathology, School of Medical Sciences, Universiti Sains Malaysia, Health Campus, Kubang Kerian 16150, Malaysia<i>Carcinoscorpius rotundicauda</i> (<i>C. rotundicauda</i>) is one of the four species of horseshoe crabs (HSCs). The HSC hemocytes store defense molecules that are released upon encountering invading pathogens. The HSCs rely on this innate immunity to continue its existence as a living fossil for more than 480 million years. To gain insight into the innate mechanisms involved, transcriptomic analysis was performed on isolated <i>C. rotundicauda</i> hemocytes challenged with lipopolysaccharides (LPS), the main components of the outer cell membrane of gram-negative bacteria. RNA-sequencing with Illumina HiSeq platform resulted in 232,628,086 and 245,448,176 raw reads corresponding to 190,326,253 and 201,180,020 high-quality mappable reads from control and LPS-stimulated hemocytes, respectively. Following LPS-stimulation, 79 genes were significantly upregulated and 265 genes were downregulated. The differentially expressed genes (DEGs) were related to multiple immune functional categories and pathways such as those of the cytoskeleton, Toll and Imd, apoptosis, MAP kinase (MAPK), inositol phosphate metabolism, phagosome, leucocyte endothelial migration, and gram-negative bacterial infection, among others. This study provides important information about the mechanisms of response to LPS, which is relevant for the understanding the HSCs’ immune response.https://www.mdpi.com/1467-3045/44/12/399<i>Carcinoscorpius rotundicauda</i>RNA-sequencinglipopolysaccharides challengeimmune response |
| spellingShingle | Maria E. Sarmiento Kai Ling Chin Nyok-Sean Lau Noraznawati Ismail Mohd Nor Norazmi Armando Acosta Nik Soriani Yaacob Transcriptomic Signature of Horseshoe Crab <i>Carcinoscorpius rotundicauda</i> Hemocytes’ Response to Lipopolysaccharides Current Issues in Molecular Biology <i>Carcinoscorpius rotundicauda</i> RNA-sequencing lipopolysaccharides challenge immune response |
| title | Transcriptomic Signature of Horseshoe Crab <i>Carcinoscorpius rotundicauda</i> Hemocytes’ Response to Lipopolysaccharides |
| title_full | Transcriptomic Signature of Horseshoe Crab <i>Carcinoscorpius rotundicauda</i> Hemocytes’ Response to Lipopolysaccharides |
| title_fullStr | Transcriptomic Signature of Horseshoe Crab <i>Carcinoscorpius rotundicauda</i> Hemocytes’ Response to Lipopolysaccharides |
| title_full_unstemmed | Transcriptomic Signature of Horseshoe Crab <i>Carcinoscorpius rotundicauda</i> Hemocytes’ Response to Lipopolysaccharides |
| title_short | Transcriptomic Signature of Horseshoe Crab <i>Carcinoscorpius rotundicauda</i> Hemocytes’ Response to Lipopolysaccharides |
| title_sort | transcriptomic signature of horseshoe crab i carcinoscorpius rotundicauda i hemocytes response to lipopolysaccharides |
| topic | <i>Carcinoscorpius rotundicauda</i> RNA-sequencing lipopolysaccharides challenge immune response |
| url | https://www.mdpi.com/1467-3045/44/12/399 |
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