Role of Plasma Membrane at Dielectric Relaxations and Intermembrane Interaction in Human Erythrocytes
Dielectric relaxations at 1.4 MHz (β<sub>sp</sub>) and 9 MHz (γ1<sub>sp</sub>) on the erythrocyte spectrin network were studied by dielectric spectroscopy using dense suspensions of erythrocytes and erythrocyte ghost membranes, subjected to extraction with up to 0.2% volume T...
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MDPI AG
2023-07-01
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| Series: | Membranes |
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| Online Access: | https://www.mdpi.com/2077-0375/13/7/658 |
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| author | Ivan T. Ivanov Boyana K. Paarvanova |
| author_facet | Ivan T. Ivanov Boyana K. Paarvanova |
| author_sort | Ivan T. Ivanov |
| collection | DOAJ |
| description | Dielectric relaxations at 1.4 MHz (β<sub>sp</sub>) and 9 MHz (γ1<sub>sp</sub>) on the erythrocyte spectrin network were studied by dielectric spectroscopy using dense suspensions of erythrocytes and erythrocyte ghost membranes, subjected to extraction with up to 0.2% volume Triton-X-100. The step-wise extraction of up to 60% of membrane lipids preserved γ1<sub>sp</sub> and gradually removed β<sub>sp</sub>-relaxation. On increasing the concentration up to 100 mM of NaCl at either side of erythrocyte plasma membranes, the β<sub>sp</sub>-relaxation was linearly enhanced, while the strength of γ1<sub>sp</sub>-relaxation remained unchanged. In media with NaCl between 100 and 150 mM β<sub>sp</sub>-relaxation became slightly inhibited, while γ1<sub>sp</sub>-relaxation almost disappeared, possibly due to the decreased electrostatic repulsion allowing erythrocytes to come into closer contact. When these media contained, at concentrations 10–30 mg/mL dextran (MW 7 kDa), polyethylene glycol or polyvinylpyrrolidone (40 kDa), or albumin or homologous plasma with equivalent concentration of albumin, the γ1<sub>sp</sub>-relaxation was about tenfold enhanced, while β<sub>sp</sub>-relaxation was strengthened or preserved. The results suggest the Maxwell–Vagner accumulation of ions on the lipid bilayer as an energy source for β<sub>sp</sub>-relaxation. While β<sub>sp</sub>-relaxation appears sensitive to erythrocyte membrane deformability, γ1<sub>sp</sub>-relaxation could be a sensitive marker for the inter-membrane interactions between erythrocytes. |
| first_indexed | 2024-03-11T00:50:53Z |
| format | Article |
| id | doaj.art-710e3b035510494aa3c282b059487786 |
| institution | Directory Open Access Journal |
| issn | 2077-0375 |
| language | English |
| last_indexed | 2024-03-11T00:50:53Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Membranes |
| spelling | doaj.art-710e3b035510494aa3c282b0594877862023-11-18T20:26:29ZengMDPI AGMembranes2077-03752023-07-0113765810.3390/membranes13070658Role of Plasma Membrane at Dielectric Relaxations and Intermembrane Interaction in Human ErythrocytesIvan T. Ivanov0Boyana K. Paarvanova1Department of Physics, Biophysics, Roentgenology and Radiology, Medical Faculty, Thracian University, 6000 Stara Zagora, BulgariaDepartment of Physics, Biophysics, Roentgenology and Radiology, Medical Faculty, Thracian University, 6000 Stara Zagora, BulgariaDielectric relaxations at 1.4 MHz (β<sub>sp</sub>) and 9 MHz (γ1<sub>sp</sub>) on the erythrocyte spectrin network were studied by dielectric spectroscopy using dense suspensions of erythrocytes and erythrocyte ghost membranes, subjected to extraction with up to 0.2% volume Triton-X-100. The step-wise extraction of up to 60% of membrane lipids preserved γ1<sub>sp</sub> and gradually removed β<sub>sp</sub>-relaxation. On increasing the concentration up to 100 mM of NaCl at either side of erythrocyte plasma membranes, the β<sub>sp</sub>-relaxation was linearly enhanced, while the strength of γ1<sub>sp</sub>-relaxation remained unchanged. In media with NaCl between 100 and 150 mM β<sub>sp</sub>-relaxation became slightly inhibited, while γ1<sub>sp</sub>-relaxation almost disappeared, possibly due to the decreased electrostatic repulsion allowing erythrocytes to come into closer contact. When these media contained, at concentrations 10–30 mg/mL dextran (MW 7 kDa), polyethylene glycol or polyvinylpyrrolidone (40 kDa), or albumin or homologous plasma with equivalent concentration of albumin, the γ1<sub>sp</sub>-relaxation was about tenfold enhanced, while β<sub>sp</sub>-relaxation was strengthened or preserved. The results suggest the Maxwell–Vagner accumulation of ions on the lipid bilayer as an energy source for β<sub>sp</sub>-relaxation. While β<sub>sp</sub>-relaxation appears sensitive to erythrocyte membrane deformability, γ1<sub>sp</sub>-relaxation could be a sensitive marker for the inter-membrane interactions between erythrocytes.https://www.mdpi.com/2077-0375/13/7/658erythrocyte membranespectrin skeletonerythrocyte aggregationdeformabilitydielectric relaxation |
| spellingShingle | Ivan T. Ivanov Boyana K. Paarvanova Role of Plasma Membrane at Dielectric Relaxations and Intermembrane Interaction in Human Erythrocytes Membranes erythrocyte membrane spectrin skeleton erythrocyte aggregation deformability dielectric relaxation |
| title | Role of Plasma Membrane at Dielectric Relaxations and Intermembrane Interaction in Human Erythrocytes |
| title_full | Role of Plasma Membrane at Dielectric Relaxations and Intermembrane Interaction in Human Erythrocytes |
| title_fullStr | Role of Plasma Membrane at Dielectric Relaxations and Intermembrane Interaction in Human Erythrocytes |
| title_full_unstemmed | Role of Plasma Membrane at Dielectric Relaxations and Intermembrane Interaction in Human Erythrocytes |
| title_short | Role of Plasma Membrane at Dielectric Relaxations and Intermembrane Interaction in Human Erythrocytes |
| title_sort | role of plasma membrane at dielectric relaxations and intermembrane interaction in human erythrocytes |
| topic | erythrocyte membrane spectrin skeleton erythrocyte aggregation deformability dielectric relaxation |
| url | https://www.mdpi.com/2077-0375/13/7/658 |
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