The zinc finger proteins ZNF644 and WIZ regulate the G9a/GLP complex for gene repression
The G9a/GLP complex mediates mono- and dimethylation of Lys9 of histone H3 at specific gene loci, which is associated with transcriptional repression. However, the molecular mechanism by which the G9a/GLP complex is targeted to the specific gene loci for H3K9 methylation is unclear. In this study, w...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2015-03-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/05606 |
| _version_ | 1811236946116083712 |
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| author | Chunjing Bian Qiang Chen Xiaochun Yu |
| author_facet | Chunjing Bian Qiang Chen Xiaochun Yu |
| author_sort | Chunjing Bian |
| collection | DOAJ |
| description | The G9a/GLP complex mediates mono- and dimethylation of Lys9 of histone H3 at specific gene loci, which is associated with transcriptional repression. However, the molecular mechanism by which the G9a/GLP complex is targeted to the specific gene loci for H3K9 methylation is unclear. In this study, with unbiased protein affinity purification, we found ZNF644 and WIZ as two core subunits in the G9a/GLP complex. ZNF644 and WIZ interact with the transcription activation domain of G9a and GLP, respectively. Moreover, both ZNF644 and WIZ contain multiple zinc finger motifs that recognize consensus DNA sequences. ZNF644 and WIZ target G9a and GLP to the chromatin and mediate the G9a/GLP complex-dependent H3K9 methylation as well as gene repression. Thus, our studies reveal two key subunits in the G9a/GLP complex that regulate the function of this histone methyltransferase complex. |
| first_indexed | 2024-04-12T12:17:51Z |
| format | Article |
| id | doaj.art-710f8a7cae0e42229a12afbc3c669ec0 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T12:17:51Z |
| publishDate | 2015-03-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-710f8a7cae0e42229a12afbc3c669ec02022-12-22T03:33:23ZengeLife Sciences Publications LtdeLife2050-084X2015-03-01410.7554/eLife.05606The zinc finger proteins ZNF644 and WIZ regulate the G9a/GLP complex for gene repressionChunjing Bian0Qiang Chen1Xiaochun Yu2Division of Molecular Medicine and Genetics, Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, United StatesDivision of Molecular Medicine and Genetics, Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, United StatesDivision of Molecular Medicine and Genetics, Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, United StatesThe G9a/GLP complex mediates mono- and dimethylation of Lys9 of histone H3 at specific gene loci, which is associated with transcriptional repression. However, the molecular mechanism by which the G9a/GLP complex is targeted to the specific gene loci for H3K9 methylation is unclear. In this study, with unbiased protein affinity purification, we found ZNF644 and WIZ as two core subunits in the G9a/GLP complex. ZNF644 and WIZ interact with the transcription activation domain of G9a and GLP, respectively. Moreover, both ZNF644 and WIZ contain multiple zinc finger motifs that recognize consensus DNA sequences. ZNF644 and WIZ target G9a and GLP to the chromatin and mediate the G9a/GLP complex-dependent H3K9 methylation as well as gene repression. Thus, our studies reveal two key subunits in the G9a/GLP complex that regulate the function of this histone methyltransferase complex.https://elifesciences.org/articles/05606G9aH3K9 methylationepigenetic modificationzinc finger motif |
| spellingShingle | Chunjing Bian Qiang Chen Xiaochun Yu The zinc finger proteins ZNF644 and WIZ regulate the G9a/GLP complex for gene repression eLife G9a H3K9 methylation epigenetic modification zinc finger motif |
| title | The zinc finger proteins ZNF644 and WIZ regulate the G9a/GLP complex for gene repression |
| title_full | The zinc finger proteins ZNF644 and WIZ regulate the G9a/GLP complex for gene repression |
| title_fullStr | The zinc finger proteins ZNF644 and WIZ regulate the G9a/GLP complex for gene repression |
| title_full_unstemmed | The zinc finger proteins ZNF644 and WIZ regulate the G9a/GLP complex for gene repression |
| title_short | The zinc finger proteins ZNF644 and WIZ regulate the G9a/GLP complex for gene repression |
| title_sort | zinc finger proteins znf644 and wiz regulate the g9a glp complex for gene repression |
| topic | G9a H3K9 methylation epigenetic modification zinc finger motif |
| url | https://elifesciences.org/articles/05606 |
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