Genome-wide analysis and molecular dissection of the SPL gene family in Fraxinus mandshurica
Abstract Background SQUAMOSA promoter binding protein-like (SPL) is a unique family of transcription factors in plants, which is engaged in regulating plant growth and development, physiological and biochemical processes. Fraxinus mandshurica is an excellent timber species with a wide range of uses...
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| Format: | Article |
| Language: | English |
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BMC
2022-09-01
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| Series: | BMC Plant Biology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12870-022-03838-9 |
| _version_ | 1828235667943981056 |
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| author | Biying He Shangzhu Gao Han Lu Jialin Yan Caihua Li Minghao Ma Xigang Wang Xiaohui Chen Yaguang Zhan Fansuo Zeng |
| author_facet | Biying He Shangzhu Gao Han Lu Jialin Yan Caihua Li Minghao Ma Xigang Wang Xiaohui Chen Yaguang Zhan Fansuo Zeng |
| author_sort | Biying He |
| collection | DOAJ |
| description | Abstract Background SQUAMOSA promoter binding protein-like (SPL) is a unique family of transcription factors in plants, which is engaged in regulating plant growth and development, physiological and biochemical processes. Fraxinus mandshurica is an excellent timber species with a wide range of uses in northeastern China and enjoys a high reputation in the international market. SPL family analysis has been reported in some plants while SPL family analysis of Fraxinus mandshurica has not been reported. Results We used phylogeny, conserved motifs, gene structure, secondary structure prediction, miR156 binding sites, promoter cis elements and GO annotation to systematically analyze the FmSPLs family. This was followed by expression analysis by subcellular localization, expression patterns at various tissue sites, abiotic stress and hormone induction. Because FmSPL2 is highly expressed in flowers it was selected to describe the SPL gene family of Fraxinus mandshurica by ectopic expression. Among them, 10 FmSPL genes that were highly expressed at different loci were selected for expression analysis under abiotic stress (NaCl and Cold) and hormone induction (IAA and ABA). These 10 FmSPL genes showed corresponding trends in response to both abiotic stress and hormone induction. We showed that overexpression of FmSPL2 in transgenic Nicotiana tabacum L. resulted in taller plants, shorter root length, increased root number, rounded leaves, and earlier flowering time. Conclusions We identified 36 SPL genes, which were classified into seven subfamilies based on sequence analysis. FmSPL2 was selected for subsequent heterologous expression by analysis of expression patterns in various tissues and under abiotic stress and hormone induction, and significant phenotypic changes were observed in the transgenic Nicotiana tabacum L. These results provide insight into the evolutionary origin and biological significance of plant SPL. The aim of this study was to lay the foundation for the genetic improvement of Fraxinus mandshurica and the subsequent functional analysis of FmSPL2. |
| first_indexed | 2024-04-12T20:18:07Z |
| format | Article |
| id | doaj.art-7111ddfde3f0480da763e1db8d9cb2e2 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-04-12T20:18:07Z |
| publishDate | 2022-09-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-7111ddfde3f0480da763e1db8d9cb2e22022-12-22T03:18:04ZengBMCBMC Plant Biology1471-22292022-09-0122111810.1186/s12870-022-03838-9Genome-wide analysis and molecular dissection of the SPL gene family in Fraxinus mandshuricaBiying He0Shangzhu Gao1Han Lu2Jialin Yan3Caihua Li4Minghao Ma5Xigang Wang6Xiaohui Chen7Yaguang Zhan8Fansuo Zeng9State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry UniversityState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry UniversityState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry UniversityState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry UniversityShijiazhuang Academy of Agricultural and Forestry SciencesCollege of Life Science, Northeast Forestry UniversityCollege of Life Science, Northeast Forestry UniversityState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry UniversityState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry UniversityState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry UniversityAbstract Background SQUAMOSA promoter binding protein-like (SPL) is a unique family of transcription factors in plants, which is engaged in regulating plant growth and development, physiological and biochemical processes. Fraxinus mandshurica is an excellent timber species with a wide range of uses in northeastern China and enjoys a high reputation in the international market. SPL family analysis has been reported in some plants while SPL family analysis of Fraxinus mandshurica has not been reported. Results We used phylogeny, conserved motifs, gene structure, secondary structure prediction, miR156 binding sites, promoter cis elements and GO annotation to systematically analyze the FmSPLs family. This was followed by expression analysis by subcellular localization, expression patterns at various tissue sites, abiotic stress and hormone induction. Because FmSPL2 is highly expressed in flowers it was selected to describe the SPL gene family of Fraxinus mandshurica by ectopic expression. Among them, 10 FmSPL genes that were highly expressed at different loci were selected for expression analysis under abiotic stress (NaCl and Cold) and hormone induction (IAA and ABA). These 10 FmSPL genes showed corresponding trends in response to both abiotic stress and hormone induction. We showed that overexpression of FmSPL2 in transgenic Nicotiana tabacum L. resulted in taller plants, shorter root length, increased root number, rounded leaves, and earlier flowering time. Conclusions We identified 36 SPL genes, which were classified into seven subfamilies based on sequence analysis. FmSPL2 was selected for subsequent heterologous expression by analysis of expression patterns in various tissues and under abiotic stress and hormone induction, and significant phenotypic changes were observed in the transgenic Nicotiana tabacum L. These results provide insight into the evolutionary origin and biological significance of plant SPL. The aim of this study was to lay the foundation for the genetic improvement of Fraxinus mandshurica and the subsequent functional analysis of FmSPL2.https://doi.org/10.1186/s12870-022-03838-9Fraxinus mandshuricaSPLGenome-widemiR156Abiotic stress |
| spellingShingle | Biying He Shangzhu Gao Han Lu Jialin Yan Caihua Li Minghao Ma Xigang Wang Xiaohui Chen Yaguang Zhan Fansuo Zeng Genome-wide analysis and molecular dissection of the SPL gene family in Fraxinus mandshurica BMC Plant Biology Fraxinus mandshurica SPL Genome-wide miR156 Abiotic stress |
| title | Genome-wide analysis and molecular dissection of the SPL gene family in Fraxinus mandshurica |
| title_full | Genome-wide analysis and molecular dissection of the SPL gene family in Fraxinus mandshurica |
| title_fullStr | Genome-wide analysis and molecular dissection of the SPL gene family in Fraxinus mandshurica |
| title_full_unstemmed | Genome-wide analysis and molecular dissection of the SPL gene family in Fraxinus mandshurica |
| title_short | Genome-wide analysis and molecular dissection of the SPL gene family in Fraxinus mandshurica |
| title_sort | genome wide analysis and molecular dissection of the spl gene family in fraxinus mandshurica |
| topic | Fraxinus mandshurica SPL Genome-wide miR156 Abiotic stress |
| url | https://doi.org/10.1186/s12870-022-03838-9 |
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