A Novel Methodology for Detecting Variations in Cell Surface Antigens Using Cell-Tearing by Optical Tweezers
The quantitative analysis of cell surface antigens has attracted increasing attention due to the antigenic variation recognition that can facilitate early diagnoses. This paper presents a novel methodology based on the optical “cell-tearing” and the especially proposed “dilution regulations” to dete...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-08-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/12/8/656 |
| _version_ | 1827600571115241472 |
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| author | Chih-Lang Lin Shyang-Guang Wang Meng-Tsung Tien Chung-Han Chiang Yi-Chieh Lee Patrice L. Baldeck Chow-Shing Shin |
| author_facet | Chih-Lang Lin Shyang-Guang Wang Meng-Tsung Tien Chung-Han Chiang Yi-Chieh Lee Patrice L. Baldeck Chow-Shing Shin |
| author_sort | Chih-Lang Lin |
| collection | DOAJ |
| description | The quantitative analysis of cell surface antigens has attracted increasing attention due to the antigenic variation recognition that can facilitate early diagnoses. This paper presents a novel methodology based on the optical “cell-tearing” and the especially proposed “dilution regulations” to detect variations in cell surface antigens. The cell attaches to the corresponding antibody-coated slide surface. Then, the cell-binding firmness between a single cell and the functionalized surface is assayed by optically tearing using gradually reduced laser powers incorporated with serial antibody dilutions. Groups B and B3 of red blood cells (RBCs) were selected as the experiment subject. The results indicate that a higher dilution called for lower power to tear off the cell binding. According to the proposed relative-quantitative analysis theory, antigenic variation can be intuitively estimated by comparing the maximum allowable dilution folds. The estimation result shows good consistency with the finding in the literature. This study suggests a novel methodology for examining the variation in cell surface antigens, expected to be widely capable with potential sensor applications not only in biochemistry and biophysics, but also in the micro-/nano- engineering field. |
| first_indexed | 2024-03-09T04:39:04Z |
| format | Article |
| id | doaj.art-7115d773e8c24c309f00a53fb7250ca4 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-09T04:39:04Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-7115d773e8c24c309f00a53fb7250ca42023-12-03T13:23:21ZengMDPI AGBiosensors2079-63742022-08-0112865610.3390/bios12080656A Novel Methodology for Detecting Variations in Cell Surface Antigens Using Cell-Tearing by Optical TweezersChih-Lang Lin0Shyang-Guang Wang1Meng-Tsung Tien2Chung-Han Chiang3Yi-Chieh Lee4Patrice L. Baldeck5Chow-Shing Shin6Graduate Institute of Biotechnology and Biomedical Engineering, Central Taiwan University of Science and Technology, Taichung City 40601, TaiwanDepartment of Medical Laboratory Science and Biotechnology, Central Taiwan University of Science and Technology, Taichung City 40601, TaiwanGeneral Education Center, Feng Chia University, Taichung City 407802, TaiwanGraduate Institute of Biotechnology and Biomedical Engineering, Central Taiwan University of Science and Technology, Taichung City 40601, TaiwanDepartment of Medical Laboratory Science and Biotechnology, Central Taiwan University of Science and Technology, Taichung City 40601, TaiwanENSL, CNRS, Laboratoire de Chimie UMR 5182, 46 allée d’Italie, 69364 Lyon, FranceDepartment of Mechanical Engineering, National Taiwan University, Taipei City 10617, TaiwanThe quantitative analysis of cell surface antigens has attracted increasing attention due to the antigenic variation recognition that can facilitate early diagnoses. This paper presents a novel methodology based on the optical “cell-tearing” and the especially proposed “dilution regulations” to detect variations in cell surface antigens. The cell attaches to the corresponding antibody-coated slide surface. Then, the cell-binding firmness between a single cell and the functionalized surface is assayed by optically tearing using gradually reduced laser powers incorporated with serial antibody dilutions. Groups B and B3 of red blood cells (RBCs) were selected as the experiment subject. The results indicate that a higher dilution called for lower power to tear off the cell binding. According to the proposed relative-quantitative analysis theory, antigenic variation can be intuitively estimated by comparing the maximum allowable dilution folds. The estimation result shows good consistency with the finding in the literature. This study suggests a novel methodology for examining the variation in cell surface antigens, expected to be widely capable with potential sensor applications not only in biochemistry and biophysics, but also in the micro-/nano- engineering field.https://www.mdpi.com/2079-6374/12/8/656antigenic variationcell surface antigensagglutination testwhole-cell-based analysisoptical tweezerscell-tearing |
| spellingShingle | Chih-Lang Lin Shyang-Guang Wang Meng-Tsung Tien Chung-Han Chiang Yi-Chieh Lee Patrice L. Baldeck Chow-Shing Shin A Novel Methodology for Detecting Variations in Cell Surface Antigens Using Cell-Tearing by Optical Tweezers Biosensors antigenic variation cell surface antigens agglutination test whole-cell-based analysis optical tweezers cell-tearing |
| title | A Novel Methodology for Detecting Variations in Cell Surface Antigens Using Cell-Tearing by Optical Tweezers |
| title_full | A Novel Methodology for Detecting Variations in Cell Surface Antigens Using Cell-Tearing by Optical Tweezers |
| title_fullStr | A Novel Methodology for Detecting Variations in Cell Surface Antigens Using Cell-Tearing by Optical Tweezers |
| title_full_unstemmed | A Novel Methodology for Detecting Variations in Cell Surface Antigens Using Cell-Tearing by Optical Tweezers |
| title_short | A Novel Methodology for Detecting Variations in Cell Surface Antigens Using Cell-Tearing by Optical Tweezers |
| title_sort | novel methodology for detecting variations in cell surface antigens using cell tearing by optical tweezers |
| topic | antigenic variation cell surface antigens agglutination test whole-cell-based analysis optical tweezers cell-tearing |
| url | https://www.mdpi.com/2079-6374/12/8/656 |
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