Normothermic Ex Vivo Heart Perfusion with Mesenchymal Stem Cell-Derived Conditioned Medium Improves Myocardial Tissue Protection in Rat Donation after Circulatory Death Hearts
Objective. Adopting hearts from donation after circulatory death (DCD) is a promising approach to enlarge the donor pool. Nevertheless, DCD hearts experience severe warm ischemia/reperfusion (I/R) injury. Recent studies have demonstrated that conditioned medium (CM) derived from bone marrow mesenchy...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Hindawi Limited
2022-01-01
|
| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2022/8513812 |
| _version_ | 1811186953560784896 |
|---|---|
| author | Zifeng Zeng Liwei Xu Yu Xu Yongsheng Ruan Deshen Liu Jiale Li Chuanjie Niu Shaoyi Zheng Pengyu Zhou Zezhou Xiao |
| author_facet | Zifeng Zeng Liwei Xu Yu Xu Yongsheng Ruan Deshen Liu Jiale Li Chuanjie Niu Shaoyi Zheng Pengyu Zhou Zezhou Xiao |
| author_sort | Zifeng Zeng |
| collection | DOAJ |
| description | Objective. Adopting hearts from donation after circulatory death (DCD) is a promising approach to enlarge the donor pool. Nevertheless, DCD hearts experience severe warm ischemia/reperfusion (I/R) injury. Recent studies have demonstrated that conditioned medium (CM) derived from bone marrow mesenchymal stem cells (BMSCs) has the potential of reducing organ I/R injury. Therefore, we investigated whether DCD heart preservation with normothermic ex vivo heart perfusion (EVHP) and BMSCs-CM treatment could alleviate myocardial warm I/R injury in the DCD hearts. Methods. We randomly divided donor rats into two groups: (1) DCD-Control group and (2) DCD-CM group. Before DCD heart preservation with the normothermic EVHP system for 105 minutes, rats suffered from a 25-minute warm ischemia injury in the DCD procedure. Vehicle or CM (300 μl) was added to the perfusate at the beginning of the perfusion process. The cardiac function of DCD hearts in the DCD-Control and DCD-CM groups was measured every 30 minutes. Besides, non-DCD hearts were harvested from the beating-heart rats. Results. The antibody array demonstrated that the CM contained 14 bioactive factors involved in apoptosis, inflammation, and oxidative stress. Warm ischemia injury resulted in a significant increase in the level of oxidative stress, inflammation, and apoptosis in the DCD hearts of DCD-Control group. Furthermore, compared with the DCD-Control group, CM treatment increased the developed pressure, dP/dtmax and dP/dtmin of the left ventricular in the DCD hearts during a 90-minute EVHP. Moreover, the administration of CM attenuated the level of oxidative stress, inflammation, and apoptosis in the DCD hearts of the DCD-CM group. Conclusions. Normothermic EVHP combined with CM treatment can alleviate warm I/R injury in the DCD hearts by decreasing the level of oxidative stress, inflammatory response, and apoptosis, which might alleviate the shortage of donor hearts by adopting DCD hearts. |
| first_indexed | 2024-04-11T13:53:43Z |
| format | Article |
| id | doaj.art-711a84f12cda4200b96d47d4ea18a1a7 |
| institution | Directory Open Access Journal |
| issn | 1687-9678 |
| language | English |
| last_indexed | 2024-04-11T13:53:43Z |
| publishDate | 2022-01-01 |
| publisher | Hindawi Limited |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj.art-711a84f12cda4200b96d47d4ea18a1a72022-12-22T04:20:26ZengHindawi LimitedStem Cells International1687-96782022-01-01202210.1155/2022/8513812Normothermic Ex Vivo Heart Perfusion with Mesenchymal Stem Cell-Derived Conditioned Medium Improves Myocardial Tissue Protection in Rat Donation after Circulatory Death HeartsZifeng Zeng0Liwei Xu1Yu Xu2Yongsheng Ruan3Deshen Liu4Jiale Li5Chuanjie Niu6Shaoyi Zheng7Pengyu Zhou8Zezhou Xiao9Department of Cardiovascular SurgeryDepartment of Cardiovascular SurgeryDepartment of Cardiovascular SurgeryDepartment of PediatricsDepartment of Cardiovascular SurgeryDepartment of Cardiovascular SurgeryDepartment of Cardiovascular SurgeryDepartment of Cardiovascular SurgeryDepartment of Cardiovascular SurgeryDepartment of Cardiovascular SurgeryObjective. Adopting hearts from donation after circulatory death (DCD) is a promising approach to enlarge the donor pool. Nevertheless, DCD hearts experience severe warm ischemia/reperfusion (I/R) injury. Recent studies have demonstrated that conditioned medium (CM) derived from bone marrow mesenchymal stem cells (BMSCs) has the potential of reducing organ I/R injury. Therefore, we investigated whether DCD heart preservation with normothermic ex vivo heart perfusion (EVHP) and BMSCs-CM treatment could alleviate myocardial warm I/R injury in the DCD hearts. Methods. We randomly divided donor rats into two groups: (1) DCD-Control group and (2) DCD-CM group. Before DCD heart preservation with the normothermic EVHP system for 105 minutes, rats suffered from a 25-minute warm ischemia injury in the DCD procedure. Vehicle or CM (300 μl) was added to the perfusate at the beginning of the perfusion process. The cardiac function of DCD hearts in the DCD-Control and DCD-CM groups was measured every 30 minutes. Besides, non-DCD hearts were harvested from the beating-heart rats. Results. The antibody array demonstrated that the CM contained 14 bioactive factors involved in apoptosis, inflammation, and oxidative stress. Warm ischemia injury resulted in a significant increase in the level of oxidative stress, inflammation, and apoptosis in the DCD hearts of DCD-Control group. Furthermore, compared with the DCD-Control group, CM treatment increased the developed pressure, dP/dtmax and dP/dtmin of the left ventricular in the DCD hearts during a 90-minute EVHP. Moreover, the administration of CM attenuated the level of oxidative stress, inflammation, and apoptosis in the DCD hearts of the DCD-CM group. Conclusions. Normothermic EVHP combined with CM treatment can alleviate warm I/R injury in the DCD hearts by decreasing the level of oxidative stress, inflammatory response, and apoptosis, which might alleviate the shortage of donor hearts by adopting DCD hearts.http://dx.doi.org/10.1155/2022/8513812 |
| spellingShingle | Zifeng Zeng Liwei Xu Yu Xu Yongsheng Ruan Deshen Liu Jiale Li Chuanjie Niu Shaoyi Zheng Pengyu Zhou Zezhou Xiao Normothermic Ex Vivo Heart Perfusion with Mesenchymal Stem Cell-Derived Conditioned Medium Improves Myocardial Tissue Protection in Rat Donation after Circulatory Death Hearts Stem Cells International |
| title | Normothermic Ex Vivo Heart Perfusion with Mesenchymal Stem Cell-Derived Conditioned Medium Improves Myocardial Tissue Protection in Rat Donation after Circulatory Death Hearts |
| title_full | Normothermic Ex Vivo Heart Perfusion with Mesenchymal Stem Cell-Derived Conditioned Medium Improves Myocardial Tissue Protection in Rat Donation after Circulatory Death Hearts |
| title_fullStr | Normothermic Ex Vivo Heart Perfusion with Mesenchymal Stem Cell-Derived Conditioned Medium Improves Myocardial Tissue Protection in Rat Donation after Circulatory Death Hearts |
| title_full_unstemmed | Normothermic Ex Vivo Heart Perfusion with Mesenchymal Stem Cell-Derived Conditioned Medium Improves Myocardial Tissue Protection in Rat Donation after Circulatory Death Hearts |
| title_short | Normothermic Ex Vivo Heart Perfusion with Mesenchymal Stem Cell-Derived Conditioned Medium Improves Myocardial Tissue Protection in Rat Donation after Circulatory Death Hearts |
| title_sort | normothermic ex vivo heart perfusion with mesenchymal stem cell derived conditioned medium improves myocardial tissue protection in rat donation after circulatory death hearts |
| url | http://dx.doi.org/10.1155/2022/8513812 |
| work_keys_str_mv | AT zifengzeng normothermicexvivoheartperfusionwithmesenchymalstemcellderivedconditionedmediumimprovesmyocardialtissueprotectioninratdonationaftercirculatorydeathhearts AT liweixu normothermicexvivoheartperfusionwithmesenchymalstemcellderivedconditionedmediumimprovesmyocardialtissueprotectioninratdonationaftercirculatorydeathhearts AT yuxu normothermicexvivoheartperfusionwithmesenchymalstemcellderivedconditionedmediumimprovesmyocardialtissueprotectioninratdonationaftercirculatorydeathhearts AT yongshengruan normothermicexvivoheartperfusionwithmesenchymalstemcellderivedconditionedmediumimprovesmyocardialtissueprotectioninratdonationaftercirculatorydeathhearts AT deshenliu normothermicexvivoheartperfusionwithmesenchymalstemcellderivedconditionedmediumimprovesmyocardialtissueprotectioninratdonationaftercirculatorydeathhearts AT jialeli normothermicexvivoheartperfusionwithmesenchymalstemcellderivedconditionedmediumimprovesmyocardialtissueprotectioninratdonationaftercirculatorydeathhearts AT chuanjieniu normothermicexvivoheartperfusionwithmesenchymalstemcellderivedconditionedmediumimprovesmyocardialtissueprotectioninratdonationaftercirculatorydeathhearts AT shaoyizheng normothermicexvivoheartperfusionwithmesenchymalstemcellderivedconditionedmediumimprovesmyocardialtissueprotectioninratdonationaftercirculatorydeathhearts AT pengyuzhou normothermicexvivoheartperfusionwithmesenchymalstemcellderivedconditionedmediumimprovesmyocardialtissueprotectioninratdonationaftercirculatorydeathhearts AT zezhouxiao normothermicexvivoheartperfusionwithmesenchymalstemcellderivedconditionedmediumimprovesmyocardialtissueprotectioninratdonationaftercirculatorydeathhearts |