Protocol for Establishing Mouse Embryonic Stem Cells to Study Histone Inheritance Pattern at Single-Cell Resolution
Summary: Asymmetric histone inheritance can regulate cell-fate determination in Drosophila male germline stem cells. However, it remains elusive how this mechanism may be used in mammalian system. Recently, we show mouse embryonic stem cells (mESCs) with Wnt3a beads display non-overlapping H3/H4 pat...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020-12-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166720301659 |
| _version_ | 1819061591366172672 |
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| author | Binbin Ma Tung-Jui Trieu Shukry J. Habib Xin Chen |
| author_facet | Binbin Ma Tung-Jui Trieu Shukry J. Habib Xin Chen |
| author_sort | Binbin Ma |
| collection | DOAJ |
| description | Summary: Asymmetric histone inheritance can regulate cell-fate determination in Drosophila male germline stem cells. However, it remains elusive how this mechanism may be used in mammalian system. Recently, we show mouse embryonic stem cells (mESCs) with Wnt3a beads display non-overlapping H3/H4 patterns. Here, we present a detailed protocol for tracking histone inheritance in asymmetrically dividing mESCs at single-cell resolution. This protocol will establish a new system to study histone inheritance in cultured mammalian cells and could be applied to other parallel systems.For complete details on the use and execution of this protocol, please refer to Tran et al. (2012), Habib et al. (2013), Lowndes et al. (2017), and Ma et al. (2020). |
| first_indexed | 2024-12-21T14:45:19Z |
| format | Article |
| id | doaj.art-711ae6d721174f59b4bb762ef112dc07 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-12-21T14:45:19Z |
| publishDate | 2020-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-711ae6d721174f59b4bb762ef112dc072022-12-21T19:00:02ZengElsevierSTAR Protocols2666-16672020-12-0113100178Protocol for Establishing Mouse Embryonic Stem Cells to Study Histone Inheritance Pattern at Single-Cell ResolutionBinbin Ma0Tung-Jui Trieu1Shukry J. Habib2Xin Chen3Department of Biology, The Johns Hopkins University, Baltimore, MD 21218, USA; Corresponding authorCentre for Stem Cells and Regenerative Medicine, King's College London, London SE1 9RT, UKCentre for Stem Cells and Regenerative Medicine, King's College London, London SE1 9RT, UKDepartment of Biology, The Johns Hopkins University, Baltimore, MD 21218, USA; Corresponding authorSummary: Asymmetric histone inheritance can regulate cell-fate determination in Drosophila male germline stem cells. However, it remains elusive how this mechanism may be used in mammalian system. Recently, we show mouse embryonic stem cells (mESCs) with Wnt3a beads display non-overlapping H3/H4 patterns. Here, we present a detailed protocol for tracking histone inheritance in asymmetrically dividing mESCs at single-cell resolution. This protocol will establish a new system to study histone inheritance in cultured mammalian cells and could be applied to other parallel systems.For complete details on the use and execution of this protocol, please refer to Tran et al. (2012), Habib et al. (2013), Lowndes et al. (2017), and Ma et al. (2020).http://www.sciencedirect.com/science/article/pii/S2666166720301659Cell BiologySingle CellStem Cells |
| spellingShingle | Binbin Ma Tung-Jui Trieu Shukry J. Habib Xin Chen Protocol for Establishing Mouse Embryonic Stem Cells to Study Histone Inheritance Pattern at Single-Cell Resolution STAR Protocols Cell Biology Single Cell Stem Cells |
| title | Protocol for Establishing Mouse Embryonic Stem Cells to Study Histone Inheritance Pattern at Single-Cell Resolution |
| title_full | Protocol for Establishing Mouse Embryonic Stem Cells to Study Histone Inheritance Pattern at Single-Cell Resolution |
| title_fullStr | Protocol for Establishing Mouse Embryonic Stem Cells to Study Histone Inheritance Pattern at Single-Cell Resolution |
| title_full_unstemmed | Protocol for Establishing Mouse Embryonic Stem Cells to Study Histone Inheritance Pattern at Single-Cell Resolution |
| title_short | Protocol for Establishing Mouse Embryonic Stem Cells to Study Histone Inheritance Pattern at Single-Cell Resolution |
| title_sort | protocol for establishing mouse embryonic stem cells to study histone inheritance pattern at single cell resolution |
| topic | Cell Biology Single Cell Stem Cells |
| url | http://www.sciencedirect.com/science/article/pii/S2666166720301659 |
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