Comparison of Single- and Mixed-Sized Gold Nanoparticles on Lateral Flow Assay for Albumin Detection
The sensitivity and reproducibility of the lateral flow assay can be influenced by multiple factors, such as the size of gold nanoparticles (GNPs) employed. Here, we evaluated the analytical performance of single-sized and mixed-sized GNPs using a simple lateral flow assay (LFA) platform. This platf...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-06-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/11/7/209 |
| _version_ | 1797411903182471168 |
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| author | Sasima Chotithammakul Michael B. Cortie Dakrong Pissuwan |
| author_facet | Sasima Chotithammakul Michael B. Cortie Dakrong Pissuwan |
| author_sort | Sasima Chotithammakul |
| collection | DOAJ |
| description | The sensitivity and reproducibility of the lateral flow assay can be influenced by multiple factors, such as the size of gold nanoparticles (GNPs) employed. Here, we evaluated the analytical performance of single-sized and mixed-sized GNPs using a simple lateral flow assay (LFA) platform. This platform was used as a model assay to diagnose albumin levels and demonstrate the analytical performance of single-sized and mixed-sized GNPs in LFA tests. Two sizes of GNPs@anti-bovine serum albumin (BSA) conjugate proteins were mixed at different ratios. The unique optical properties of the GNPs induced a distinguishing color-shedding effect on the single- and mixed-sized GNPs@anti-BSA conjugates interacting with the target analyte BSA spotted on the test line. The use of mixed-sized GNPs@anti-BSA conjugates enhanced signal relative to the 20 nm GNPs, and provided superior stability compared with solely employing the large GNPs (50 nm). The proposed platform in this study could provide an efficient BSA detection mechanism that can be utilized as a model biomarker for confronting chronic kidney disease. |
| first_indexed | 2024-03-09T04:53:04Z |
| format | Article |
| id | doaj.art-711bb117665c4c11a5675de543f5a6d8 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-09T04:53:04Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-711bb117665c4c11a5675de543f5a6d82023-12-03T13:08:19ZengMDPI AGBiosensors2079-63742021-06-0111720910.3390/bios11070209Comparison of Single- and Mixed-Sized Gold Nanoparticles on Lateral Flow Assay for Albumin DetectionSasima Chotithammakul0Michael B. Cortie1Dakrong Pissuwan2Materials Science and Engineering Program, Faculty of Science, Mahidol University, Bangkok 10400, ThailandSchool of Mathematical and Physical Sciences, University of Technology Sydney, Broadway, NSW 2007, AustraliaMaterials Science and Engineering Program, Faculty of Science, Mahidol University, Bangkok 10400, ThailandThe sensitivity and reproducibility of the lateral flow assay can be influenced by multiple factors, such as the size of gold nanoparticles (GNPs) employed. Here, we evaluated the analytical performance of single-sized and mixed-sized GNPs using a simple lateral flow assay (LFA) platform. This platform was used as a model assay to diagnose albumin levels and demonstrate the analytical performance of single-sized and mixed-sized GNPs in LFA tests. Two sizes of GNPs@anti-bovine serum albumin (BSA) conjugate proteins were mixed at different ratios. The unique optical properties of the GNPs induced a distinguishing color-shedding effect on the single- and mixed-sized GNPs@anti-BSA conjugates interacting with the target analyte BSA spotted on the test line. The use of mixed-sized GNPs@anti-BSA conjugates enhanced signal relative to the 20 nm GNPs, and provided superior stability compared with solely employing the large GNPs (50 nm). The proposed platform in this study could provide an efficient BSA detection mechanism that can be utilized as a model biomarker for confronting chronic kidney disease.https://www.mdpi.com/2079-6374/11/7/209gold nanoparticlessingle sizemixed sizeslateral flow assayalbumin |
| spellingShingle | Sasima Chotithammakul Michael B. Cortie Dakrong Pissuwan Comparison of Single- and Mixed-Sized Gold Nanoparticles on Lateral Flow Assay for Albumin Detection Biosensors gold nanoparticles single size mixed sizes lateral flow assay albumin |
| title | Comparison of Single- and Mixed-Sized Gold Nanoparticles on Lateral Flow Assay for Albumin Detection |
| title_full | Comparison of Single- and Mixed-Sized Gold Nanoparticles on Lateral Flow Assay for Albumin Detection |
| title_fullStr | Comparison of Single- and Mixed-Sized Gold Nanoparticles on Lateral Flow Assay for Albumin Detection |
| title_full_unstemmed | Comparison of Single- and Mixed-Sized Gold Nanoparticles on Lateral Flow Assay for Albumin Detection |
| title_short | Comparison of Single- and Mixed-Sized Gold Nanoparticles on Lateral Flow Assay for Albumin Detection |
| title_sort | comparison of single and mixed sized gold nanoparticles on lateral flow assay for albumin detection |
| topic | gold nanoparticles single size mixed sizes lateral flow assay albumin |
| url | https://www.mdpi.com/2079-6374/11/7/209 |
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