10-mer and 9-mer WALK Peptides with Both Antibacterial and Anti-Inflammatory Activities
Natural antimicrobial peptides (AMPs) are multifunctional host defense peptides (HDPs) that are valuable for various therapeutic applications. In particular, natural and artificial AMPs with dual antibacterial immunomodulatory functions emerged as promising candidates for the development of therapeu...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-11-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/11/11/1588 |
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| author | Su-Jin Kim Tae-Bong Kang Dong-Hyuk Kim Minho Keum Sung-Hee Lee Ji-Hun Kim Sang-Hyuck Lee Jihoon Kim Hyuk-Jung Kweon Jae-Won Park Beom-Jun Kim Hyung-Sik Won |
| author_facet | Su-Jin Kim Tae-Bong Kang Dong-Hyuk Kim Minho Keum Sung-Hee Lee Ji-Hun Kim Sang-Hyuck Lee Jihoon Kim Hyuk-Jung Kweon Jae-Won Park Beom-Jun Kim Hyung-Sik Won |
| author_sort | Su-Jin Kim |
| collection | DOAJ |
| description | Natural antimicrobial peptides (AMPs) are multifunctional host defense peptides (HDPs) that are valuable for various therapeutic applications. In particular, natural and artificial AMPs with dual antibacterial immunomodulatory functions emerged as promising candidates for the development of therapeutic agents to treat infectious inflammation. In an effort to develop useful AMP variants with short lengths and simple amino acid composition, we devised a de novo design strategy to generate a series of model peptide isomer sequences, named WALK peptides, i.e., tryptophan (W)-containing amphipathic-helical (A) leucine (L)/lysine (K) peptides. Here, we generated two groups of WALK peptide isomers: W<sub>2</sub>L<sub>4</sub>K<sub>4</sub> (WALK244.01~WALK244.10) and W<sub>2</sub>L<sub>4</sub>K<sub>3</sub> (WALK243.01~WALK243.09). Most showed apparent antibacterial activities against both Gram-positive and Gram-negative bacteria at a concentration of approximately 4 μg/mL along with varied hemolytic activities against human red blood cells. In addition, some exhibited significant anti-inflammatory activities without any significant cytotoxicity in macrophages. Collectively, these results suggest that the two selected peptides, WALK244.04 and WALK243.04, showed promise for the development of antibacterial and anti-inflammatory agents. |
| first_indexed | 2024-03-09T19:19:06Z |
| format | Article |
| id | doaj.art-711c07bf8cab407db2fb0311a2a4cba3 |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-09T19:19:06Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-711c07bf8cab407db2fb0311a2a4cba32023-11-24T03:28:49ZengMDPI AGAntibiotics2079-63822022-11-011111158810.3390/antibiotics1111158810-mer and 9-mer WALK Peptides with Both Antibacterial and Anti-Inflammatory ActivitiesSu-Jin Kim0Tae-Bong Kang1Dong-Hyuk Kim2Minho Keum3Sung-Hee Lee4Ji-Hun Kim5Sang-Hyuck Lee6Jihoon Kim7Hyuk-Jung Kweon8Jae-Won Park9Beom-Jun Kim10Hyung-Sik Won11BK21 Project Team, Department of Applied Life Science, Graduate School, Konkuk University, Chungju 27478, KoreaBK21 Project Team, Department of Applied Life Science, Graduate School, Konkuk University, Chungju 27478, KoreaBK21 Project Team, Department of Applied Life Science, Graduate School, Konkuk University, Chungju 27478, KoreaBK21 Project Team, Department of Applied Life Science, Graduate School, Konkuk University, Chungju 27478, KoreaCollege of Pharmacy, Chungbuk National University, Cheongju 28160, KoreaCollege of Pharmacy, Chungbuk National University, Cheongju 28160, KoreaBK21 Project Team, Department of Applied Life Science, Graduate School, Konkuk University, Chungju 27478, KoreaBK21 Project Team, Department of Applied Life Science, Graduate School, Konkuk University, Chungju 27478, KoreaDepartment of Family Medicine, School of Medicine, Konkuk University, Chungju 27478, KoreaResearch Institute, EYESEL Co., Ltd., Yongin 16950, KoreaResearch Institute, EYESEL Co., Ltd., Yongin 16950, KoreaBK21 Project Team, Department of Applied Life Science, Graduate School, Konkuk University, Chungju 27478, KoreaNatural antimicrobial peptides (AMPs) are multifunctional host defense peptides (HDPs) that are valuable for various therapeutic applications. In particular, natural and artificial AMPs with dual antibacterial immunomodulatory functions emerged as promising candidates for the development of therapeutic agents to treat infectious inflammation. In an effort to develop useful AMP variants with short lengths and simple amino acid composition, we devised a de novo design strategy to generate a series of model peptide isomer sequences, named WALK peptides, i.e., tryptophan (W)-containing amphipathic-helical (A) leucine (L)/lysine (K) peptides. Here, we generated two groups of WALK peptide isomers: W<sub>2</sub>L<sub>4</sub>K<sub>4</sub> (WALK244.01~WALK244.10) and W<sub>2</sub>L<sub>4</sub>K<sub>3</sub> (WALK243.01~WALK243.09). Most showed apparent antibacterial activities against both Gram-positive and Gram-negative bacteria at a concentration of approximately 4 μg/mL along with varied hemolytic activities against human red blood cells. In addition, some exhibited significant anti-inflammatory activities without any significant cytotoxicity in macrophages. Collectively, these results suggest that the two selected peptides, WALK244.04 and WALK243.04, showed promise for the development of antibacterial and anti-inflammatory agents.https://www.mdpi.com/2079-6382/11/11/1588antimicrobial peptideshost defense peptidesde novo designWALK peptidesantibacterial activityanti-inflammatory activity |
| spellingShingle | Su-Jin Kim Tae-Bong Kang Dong-Hyuk Kim Minho Keum Sung-Hee Lee Ji-Hun Kim Sang-Hyuck Lee Jihoon Kim Hyuk-Jung Kweon Jae-Won Park Beom-Jun Kim Hyung-Sik Won 10-mer and 9-mer WALK Peptides with Both Antibacterial and Anti-Inflammatory Activities Antibiotics antimicrobial peptides host defense peptides de novo design WALK peptides antibacterial activity anti-inflammatory activity |
| title | 10-mer and 9-mer WALK Peptides with Both Antibacterial and Anti-Inflammatory Activities |
| title_full | 10-mer and 9-mer WALK Peptides with Both Antibacterial and Anti-Inflammatory Activities |
| title_fullStr | 10-mer and 9-mer WALK Peptides with Both Antibacterial and Anti-Inflammatory Activities |
| title_full_unstemmed | 10-mer and 9-mer WALK Peptides with Both Antibacterial and Anti-Inflammatory Activities |
| title_short | 10-mer and 9-mer WALK Peptides with Both Antibacterial and Anti-Inflammatory Activities |
| title_sort | 10 mer and 9 mer walk peptides with both antibacterial and anti inflammatory activities |
| topic | antimicrobial peptides host defense peptides de novo design WALK peptides antibacterial activity anti-inflammatory activity |
| url | https://www.mdpi.com/2079-6382/11/11/1588 |
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