Collagen Expression in Various Degenerative Meniscal Changes: An Immunohistological Study
Purpose. To examine changes in acid mucopolysaccharides and collagen expression during meniscal degeneration, tearing, and repair, using menisci excised from knee joint surgeries. Methods. Menisci excised from 23 patients aged 15 to 80 years who underwent meniscal surgery for flap and bucket handle...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2013-08-01
|
| Series: | Journal of Orthopaedic Surgery |
| Online Access: | https://doi.org/10.1177/230949901302100221 |
| _version_ | 1818159831610032128 |
|---|---|
| author | Takatomo Mine Koichiro Ihara Hiroyuki Kawamura Ryo Date Keitaro Umehara |
| author_facet | Takatomo Mine Koichiro Ihara Hiroyuki Kawamura Ryo Date Keitaro Umehara |
| author_sort | Takatomo Mine |
| collection | DOAJ |
| description | Purpose. To examine changes in acid mucopolysaccharides and collagen expression during meniscal degeneration, tearing, and repair, using menisci excised from knee joint surgeries. Methods. Menisci excised from 23 patients aged 15 to 80 years who underwent meniscal surgery for flap and bucket handle tears (n=11) and total knee arthroplasty (TKA) for osteoarthritis (n=12) were examined histologically. Staining images were converted to greyscale images to measure the mean grey levels, which indicated densitometry. Comparisons were made between acutely injured menisci and menisci with and without degeneration (from patients with osteoarthritis) in terms of acid mucopolysaccharides, collagen types I, II, and III expression. Results. In menisci with no degeneration, acid mucopolysaccharides, collagen types I and II were expressed throughout the entire meniscus except for the circulating area. Collagen type III was intensely expressed at the exterior peripheral border and on the surface. During progression of meniscal degeneration, the expression of acid mucopolysaccharides increased, and the expression of collagen types I, II, and III decreased. In acutely injured menisci, collagen types II and III disappeared first, followed by collagen type I, resulting in the abrogation of fibre construction. Conclusion. In normal menisci, acid mucopolysaccharides and collagen types I, II, and III were well-balanced, and meniscal function was maintained. When the limits of repair were exceeded, the meniscus tissue deteriorated owing to the disappearance of collagen types II and III and a decrease in collagen type I, resulting in the abrogation of meniscus fabric construction. |
| first_indexed | 2024-12-11T15:52:14Z |
| format | Article |
| id | doaj.art-711ec23a56dc447f834f37bb91a3856c |
| institution | Directory Open Access Journal |
| issn | 2309-4990 |
| language | English |
| last_indexed | 2024-12-11T15:52:14Z |
| publishDate | 2013-08-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Journal of Orthopaedic Surgery |
| spelling | doaj.art-711ec23a56dc447f834f37bb91a3856c2022-12-22T00:59:33ZengSAGE PublishingJournal of Orthopaedic Surgery2309-49902013-08-012110.1177/230949901302100221Collagen Expression in Various Degenerative Meniscal Changes: An Immunohistological StudyTakatomo MineKoichiro IharaHiroyuki KawamuraRyo DateKeitaro UmeharaPurpose. To examine changes in acid mucopolysaccharides and collagen expression during meniscal degeneration, tearing, and repair, using menisci excised from knee joint surgeries. Methods. Menisci excised from 23 patients aged 15 to 80 years who underwent meniscal surgery for flap and bucket handle tears (n=11) and total knee arthroplasty (TKA) for osteoarthritis (n=12) were examined histologically. Staining images were converted to greyscale images to measure the mean grey levels, which indicated densitometry. Comparisons were made between acutely injured menisci and menisci with and without degeneration (from patients with osteoarthritis) in terms of acid mucopolysaccharides, collagen types I, II, and III expression. Results. In menisci with no degeneration, acid mucopolysaccharides, collagen types I and II were expressed throughout the entire meniscus except for the circulating area. Collagen type III was intensely expressed at the exterior peripheral border and on the surface. During progression of meniscal degeneration, the expression of acid mucopolysaccharides increased, and the expression of collagen types I, II, and III decreased. In acutely injured menisci, collagen types II and III disappeared first, followed by collagen type I, resulting in the abrogation of fibre construction. Conclusion. In normal menisci, acid mucopolysaccharides and collagen types I, II, and III were well-balanced, and meniscal function was maintained. When the limits of repair were exceeded, the meniscus tissue deteriorated owing to the disappearance of collagen types II and III and a decrease in collagen type I, resulting in the abrogation of meniscus fabric construction.https://doi.org/10.1177/230949901302100221 |
| spellingShingle | Takatomo Mine Koichiro Ihara Hiroyuki Kawamura Ryo Date Keitaro Umehara Collagen Expression in Various Degenerative Meniscal Changes: An Immunohistological Study Journal of Orthopaedic Surgery |
| title | Collagen Expression in Various Degenerative Meniscal Changes: An Immunohistological Study |
| title_full | Collagen Expression in Various Degenerative Meniscal Changes: An Immunohistological Study |
| title_fullStr | Collagen Expression in Various Degenerative Meniscal Changes: An Immunohistological Study |
| title_full_unstemmed | Collagen Expression in Various Degenerative Meniscal Changes: An Immunohistological Study |
| title_short | Collagen Expression in Various Degenerative Meniscal Changes: An Immunohistological Study |
| title_sort | collagen expression in various degenerative meniscal changes an immunohistological study |
| url | https://doi.org/10.1177/230949901302100221 |
| work_keys_str_mv | AT takatomomine collagenexpressioninvariousdegenerativemeniscalchangesanimmunohistologicalstudy AT koichiroihara collagenexpressioninvariousdegenerativemeniscalchangesanimmunohistologicalstudy AT hiroyukikawamura collagenexpressioninvariousdegenerativemeniscalchangesanimmunohistologicalstudy AT ryodate collagenexpressioninvariousdegenerativemeniscalchangesanimmunohistologicalstudy AT keitaroumehara collagenexpressioninvariousdegenerativemeniscalchangesanimmunohistologicalstudy |