Elucidating the Histone Deacetylase Gene Expression Signatures in Peripheral Blood Mononuclear Cells That Correlate Essential Cardiac Function and Aid in Classifying Coronary Artery Disease through a Logistic Regression Model
A proinflammatory role of <i>HDACs</i> has been implicated in the pathogenesis of atherosclerosis as an emerging novel epigenetic diagnostic biomarker. However, its association with the clinical and cardiovascular function in coronary artery disease is largely unknown. The study aimed to...
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| Format: | Article |
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MDPI AG
2023-11-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/11/11/2952 |
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| author | K. Monisha S. Mahema M. Chokkalingam Sheikh F. Ahmad Talha Bin Emran Paramasivam Prabu Shiek S. S. J. Ahmed |
| author_facet | K. Monisha S. Mahema M. Chokkalingam Sheikh F. Ahmad Talha Bin Emran Paramasivam Prabu Shiek S. S. J. Ahmed |
| author_sort | K. Monisha |
| collection | DOAJ |
| description | A proinflammatory role of <i>HDACs</i> has been implicated in the pathogenesis of atherosclerosis as an emerging novel epigenetic diagnostic biomarker. However, its association with the clinical and cardiovascular function in coronary artery disease is largely unknown. The study aimed to profile the gene expression of <i>HDAC1</i>–<i>11</i> in human peripheral blood mononuclear cells and to evaluate their influence on hematological, biochemical, and two-dimensional echocardiographic indices in CAD. The <i>HDAC</i> gene expression profiles were assessed in 62 angioproven CAD patients and compared with 62 healthy controls. Among the <i>HDACs</i>, upregulated <i>HDACs 1,2</i>, <i>4</i>, <i>6</i>, <i>8</i>, <i>9</i>, and <i>11</i> were upregulated, and <i>HDAC3</i> was downregulated, which was significantly (<i>p</i> ≤ 0.05) linked with the hematological (basophils, lymphocytes, monocytes, and neutrophils), biochemical (LDL, HDL, and TGL), and echocardiographic parameters (cardiac function: biplane LVEF, GLS, MV E/A, IVRT, and PV S/D) in CAD. Furthermore, our constructed diagnostic model with the crucial <i>HDACs</i> establishes the most crucial <i>HDACs</i> in the classification of CAD from control with an excellent accuracy of 88.6%. Conclusively, our study has provided a novel perspective on the <i>HDAC</i> gene expression underlying cardiac function that is useful in developing molecular methods for CAD diagnosis. |
| first_indexed | 2024-03-09T17:00:38Z |
| format | Article |
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| language | English |
| last_indexed | 2024-03-09T17:00:38Z |
| publishDate | 2023-11-01 |
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| series | Biomedicines |
| spelling | doaj.art-711f87ca25c64061bfb73695829f75552023-11-24T14:30:54ZengMDPI AGBiomedicines2227-90592023-11-011111295210.3390/biomedicines11112952Elucidating the Histone Deacetylase Gene Expression Signatures in Peripheral Blood Mononuclear Cells That Correlate Essential Cardiac Function and Aid in Classifying Coronary Artery Disease through a Logistic Regression ModelK. Monisha0S. Mahema1M. Chokkalingam2Sheikh F. Ahmad3Talha Bin Emran4Paramasivam Prabu5Shiek S. S. J. Ahmed6Drug Discovery and Multi-omics Laboratory, Faculty of Allied Health Sciences, Chettinad Academy of Research and Education, Chettinad Hospital and Research Institute, Kelambakkam 603103, IndiaDrug Discovery and Multi-omics Laboratory, Faculty of Allied Health Sciences, Chettinad Academy of Research and Education, Chettinad Hospital and Research Institute, Kelambakkam 603103, IndiaDepartment of Cardiology, Chettinad Hospital and Research Institute, Chettinad Health City, Kelambakkam 603103, IndiaDepartment of Pharmacology and Toxicology, College of Pharmacy, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Pathology and Laboratory Medicine, Warren Alpert Medical School, Brown University, Providence, RI 02912, USAMadras Diabetes Research Foundation, Chennai 600086, IndiaDrug Discovery and Multi-omics Laboratory, Faculty of Allied Health Sciences, Chettinad Academy of Research and Education, Chettinad Hospital and Research Institute, Kelambakkam 603103, IndiaA proinflammatory role of <i>HDACs</i> has been implicated in the pathogenesis of atherosclerosis as an emerging novel epigenetic diagnostic biomarker. However, its association with the clinical and cardiovascular function in coronary artery disease is largely unknown. The study aimed to profile the gene expression of <i>HDAC1</i>–<i>11</i> in human peripheral blood mononuclear cells and to evaluate their influence on hematological, biochemical, and two-dimensional echocardiographic indices in CAD. The <i>HDAC</i> gene expression profiles were assessed in 62 angioproven CAD patients and compared with 62 healthy controls. Among the <i>HDACs</i>, upregulated <i>HDACs 1,2</i>, <i>4</i>, <i>6</i>, <i>8</i>, <i>9</i>, and <i>11</i> were upregulated, and <i>HDAC3</i> was downregulated, which was significantly (<i>p</i> ≤ 0.05) linked with the hematological (basophils, lymphocytes, monocytes, and neutrophils), biochemical (LDL, HDL, and TGL), and echocardiographic parameters (cardiac function: biplane LVEF, GLS, MV E/A, IVRT, and PV S/D) in CAD. Furthermore, our constructed diagnostic model with the crucial <i>HDACs</i> establishes the most crucial <i>HDACs</i> in the classification of CAD from control with an excellent accuracy of 88.6%. Conclusively, our study has provided a novel perspective on the <i>HDAC</i> gene expression underlying cardiac function that is useful in developing molecular methods for CAD diagnosis.https://www.mdpi.com/2227-9059/11/11/2952<i>HDAC</i> profilingatherosclerosisepigenetic biomarkerscoronary artery diseaseleft ventricular structural indices |
| spellingShingle | K. Monisha S. Mahema M. Chokkalingam Sheikh F. Ahmad Talha Bin Emran Paramasivam Prabu Shiek S. S. J. Ahmed Elucidating the Histone Deacetylase Gene Expression Signatures in Peripheral Blood Mononuclear Cells That Correlate Essential Cardiac Function and Aid in Classifying Coronary Artery Disease through a Logistic Regression Model Biomedicines <i>HDAC</i> profiling atherosclerosis epigenetic biomarkers coronary artery disease left ventricular structural indices |
| title | Elucidating the Histone Deacetylase Gene Expression Signatures in Peripheral Blood Mononuclear Cells That Correlate Essential Cardiac Function and Aid in Classifying Coronary Artery Disease through a Logistic Regression Model |
| title_full | Elucidating the Histone Deacetylase Gene Expression Signatures in Peripheral Blood Mononuclear Cells That Correlate Essential Cardiac Function and Aid in Classifying Coronary Artery Disease through a Logistic Regression Model |
| title_fullStr | Elucidating the Histone Deacetylase Gene Expression Signatures in Peripheral Blood Mononuclear Cells That Correlate Essential Cardiac Function and Aid in Classifying Coronary Artery Disease through a Logistic Regression Model |
| title_full_unstemmed | Elucidating the Histone Deacetylase Gene Expression Signatures in Peripheral Blood Mononuclear Cells That Correlate Essential Cardiac Function and Aid in Classifying Coronary Artery Disease through a Logistic Regression Model |
| title_short | Elucidating the Histone Deacetylase Gene Expression Signatures in Peripheral Blood Mononuclear Cells That Correlate Essential Cardiac Function and Aid in Classifying Coronary Artery Disease through a Logistic Regression Model |
| title_sort | elucidating the histone deacetylase gene expression signatures in peripheral blood mononuclear cells that correlate essential cardiac function and aid in classifying coronary artery disease through a logistic regression model |
| topic | <i>HDAC</i> profiling atherosclerosis epigenetic biomarkers coronary artery disease left ventricular structural indices |
| url | https://www.mdpi.com/2227-9059/11/11/2952 |
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