| Summary: | Contamination of retail meat with extended spectrum beta-lactamase (ESBL) and/or AmpC type beta-lactamase (AmpC) producing
Escherichia coli may contribute to increased incidences of infections in humans. Regular monitoring of these bacteria is required in the view
of one health approach. In this study, 100 chicken meat samples obtained from Kars, Turkey were analysed and 214 isolates recovered from
72/100 samples were identified as E. coli. Antibiotic susceptibility tests showed that 15.42% of isolates (33/214) were resistant to at least one
cephalosporin antibiotic, 20 (9.34%) were beta-lactamase producer. Among beta-lactamase producing E. coli isolates 8 had blaTEM, 7 had
blaCTX-M and 3 had blaSHV genes. Plasmid-mediated AmpC beta-lactamase (blaCMY) gene was present in 13 isolates. Plasmid mediated quinolone
resistance genes were also screened by polymerase chain reaction and identified by sequencing of the isolates. As a result, 3 isolates were
found to be positive for qnrB, whereas the qnrS gene was detected in 4 isolates. Regarding the virulence genes 19 isolates were positive for
fimH and 2 isolates were carrying kpsMT II. Phylo-group D2, A0, A1, B1 and D1 were detected in 13, 2, 2, 2 and 1 isolates, respectively. Our
findings indicate that poultry meat could be an important carrier of ESBL positive E. coli.
|
|---|